Detection of intact insulin analogues in post‐mortem vitreous humour—Application to forensic toxicology casework

2020 ◽  
Author(s):  
Nicola Beckett ◽  
Rebecca Tidy ◽  
Bianca Douglas ◽  
Colin Priddis
Keyword(s):  
Forensic Toxicology ◽  
Insulin Analogues ◽  
Vitreous Humour ◽  
Post Mortem

Vitreous humour as a complementary sample to blood for the detection/confirmation of diazepam: ante-mortem and post-mortem studies in an animal model

2004 ◽  
Vol 23 (12) ◽  
pp. 571-577 ◽  
Author(s):  
H M Teixeira ◽  
F Reis ◽  
P Proença ◽  
P Ramos ◽  
O Quińtela ◽  
...  
Keyword(s):  
Drug Administration ◽  
Plasma Concentrations ◽  
Forensic Toxicology ◽  
Vitreous Humour ◽  
Toxic Substances ◽  
Time Of Death ◽  
Post Mortem ◽  
Correlation Ratio ◽  
Animal Group ◽  
Toxic Dose

The quantification of medical or toxic substances in vitreous humour (VH) could be very useful in forensic toxicology when blood sample determinations are impossible due to absence or deterioration. However, few studies have been made in this area and even fewer have tried to find a relationship between drug levels in both samples. To determine a correlation ratio between blood and VH diazepam (DZ) levels, we performed an experimental study using rabbits administered with a sub-toxic dose of DZ under known and controlled conditions. Blood and VH samples were collected 0.5, 1, 2, 3 and 6 hours after the drug administration in order to determine DZ and its main active metabolite, desmethyldiazepam (DMD). In addition, we have studied an animal group sacrificed 2 hours after intramuscular (i.m.) drug administration with blood and VH collection 24 hours later, to evaluate the existence of possible post-mortem changes. After DZ administration, a fast absorption phase was observed with a plasma Cmax value 1 hour after, followed by a rapid concentration decrease, with a half-life of 1 hour, indicating that, besides elimination, a fast distribution to other organs and tissues and/or hepatic metabolism occurred. Diazepam Cmax value in VH was achieved between 1 and 2 hours, when plasma concentrations had already decreased to half the value. The plasma/VH DZ ratio calculated at this time was 10. In the post-mortem study, while plasma DZ concentration at 24 hours was smaller, DMD levels were higher than those at the time of death. In the VH, both DZ and DMD concentrations at 24 hours were higher than those obtained at the time of death. That is, in both fluids DZ and DMD concentrations were different from those at the time of death and post-mortem distribution and redistribution phenomena occurred. The combination of antemortem and post-mortem studies has allowed the determination of a correlation ratio for DZ in the rabbit of 6×, comparing the concentrations in VH collected 24 hours after death with the concentrations detected in plasma at the time of death. This study opens new perspectives for the use of VH as a complementary sample to blood for DZ detection and confirmation. The putative relevance of the correlation ratio obtained, for forensic toxicology practice with medical substances, namely benzodiazepines, recommends further studies in humans.

Post-mortem vitreous humour as potential specimen for detection of insulin analogues by LC–MS/MS

2013 ◽  
Vol 233 (1-3) ◽  
pp. 328-332 ◽  
Author(s):  
Ilkka Ojanperä ◽  
Antti Sajantila ◽  
Larisa Vinogradova ◽  
Andreas Thomas ◽  
Wilhelm Schänzer ◽  
...  
Keyword(s):  
Insulin Analogues ◽  
Vitreous Humour ◽  
Post Mortem

Use of post mortem vitreous humour electrolytes in diagnosing salt water drowning deaths

Pathology ◽  
2018 ◽  
Vol 50 ◽  
pp. S97
Author(s):  
Jack Garland ◽  
Rexson Tse ◽  
S. Anne ◽  
C. Oldmeadow ◽  
J. Attia ◽  
...  
Keyword(s):  
Salt Water ◽  
Vitreous Humour ◽  
Post Mortem

Estimation of post-mortem interval: A comparison between cerebrospinal fluid and vitreous humour chemistry

2015 ◽  
Vol 36 ◽  
pp. 144-148 ◽  
Author(s):  
Rajanikanta Swain ◽  
Adarsh Kumar ◽  
Jyotiranjan Sahoo ◽  
R. Lakshmy ◽  
S.K. Gupta ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Vitreous Humour ◽  
Post Mortem ◽  
Post Mortem Interval

Magnesium, potassium, sodium and calcium in post-mortem vitreous humour from humans

1985 ◽  
Vol 27 (1) ◽  
pp. 1-13 ◽  
Author(s):  
J.G. Farmer ◽  
F. Benomran ◽  
A.A. Watson ◽  
W.A. Harland
Keyword(s):  
Vitreous Humour ◽  
Post Mortem ◽  
Potassium Sodium

Estimation of Post Mortem Interval by Means of Changes in the Eye-vitreous Humour Potassium Levels

2013 ◽  
Vol 7 (2) ◽  
pp. 232
Author(s):  
Deokar Ravindra ◽  
Shendarkar Ajay
Keyword(s):  
Vitreous Humour ◽  
Post Mortem ◽  
Post Mortem Interval

scholarly journals Skeletal tissue, a viable option in forensic toxicology? A view into post mortem cases

2020 ◽  
Vol 309 ◽  
pp. 110225
Author(s):  
Michiel Vandenbosch ◽  
Lukas Rooseleers ◽  
Wouter Van Den Bogaert ◽  
Joke Wuestenbergs ◽  
Wim Van de Voorde ◽  
...  
Keyword(s):  
Forensic Toxicology ◽  
Post Mortem ◽  
Viable Option ◽  
Skeletal Tissue
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