Temporal and spatial localization of prothymosin ? transcript in the Harderian gland of the frog,Rana esculenta

2002 ◽  
Vol 292 (7) ◽  
pp. 633-639 ◽  
Author(s):  
Gianluca de Rienzo ◽  
Rona di Sena ◽  
Diana Ferrara ◽  
Carmela Palmiero ◽  
Gabriella Chieffi Baccari ◽  
...  
Keyword(s):  
Spatial Localization ◽  
Harderian Gland ◽  
Rana Esculenta ◽  
Temporal And Spatial

A new sex dimorphism in the Harderian gland of the frog Rana esculenta

2007 ◽  
Vol 85 (8) ◽  
pp. 909-915 ◽  
Author(s):  
Ismene Serino ◽  
Gaia Izzo ◽  
Diana Ferrara ◽  
Michela d’Istria ◽  
Sergio Minucci
Keyword(s):  
Sexual Dimorphism ◽  
Secretory Activity ◽  
Harderian Gland ◽  
Polymerase Chain Reaction Analysis ◽  
Rana Esculenta ◽  
Rt Pcr ◽  
Polymerase Chain ◽  
Two Peaks ◽  
Constant Expression

The Harderian gland (Hg), the only gland found in the orbit of the frog Rana esculenta L., 1758, probably plays a role in orbital lubrication. The secretory activity of the Hg is seasonal, showing the highest activity in summer. There is little information on Hg gene expression; previously, we identified a mRNA named harderin, whose deduced protein has no homology with other proteins. Differential expression of the harderin transcript between the sexes expressed during the annual cycle implies sexual dimorphism. RT–PCR (reverse transcription – polymerase chain reaction) analysis, revealed that harderin is expressed during the entire year in the Hg of both sexes. It shows a higher level of expression in the female glands than that of male glands. Two peaks of expression, in February and in June, were observed in the female glands, while only the February peak was observed in those of males. These observations were supported by in situ hybridization. Experiments involving gonadectomy and (or) hormonal replacement therapy showed a significant decrease in harderin in the Hg of females; this effect is prevented by estradiol (testosterone had no effect), while ICI (antiestrogen) counteracts the hormonal prevention, suggesting that this sexual dimorphism is under estradiol control. The constant expression of harderin mRNA during the year suggests a probable constitutive role for this molecule.

scholarly journals Temporal and Spatial Localization of Novel Nuclear Protein NP95 in Mitotic and Meiotic Cells.

2000 ◽  
Vol 25 (3) ◽  
pp. 149-159 ◽  
Author(s):  
Takatoshi Uemura ◽  
Eiko Kubo ◽  
Yasuyoshi Kanari ◽  
Toshimiti Ikemura ◽  
Kouichi Tatsumi ◽  
...  
Keyword(s):  
Nuclear Protein ◽  
Spatial Localization ◽  
Temporal And Spatial

scholarly journals Myosin V regulates spatial localization of different forms of neurotransmitter release in central synapses

2020 ◽  
Author(s):  
Dario Maschi ◽  
Michael W. Gramlich ◽  
Vitaly A. Klyachko
Keyword(s):  
Neurotransmitter Release ◽  
Molecular Motor ◽  
Spatial Localization ◽  
Myosin V ◽  
Vesicle Docking ◽  
Spatial Shift ◽  
Spatially Distributed ◽  
Temporal And Spatial ◽  
Preferential Location ◽  
Central Synapses

SUMMARYSynaptic active zone (AZ) contains multiple specialized release sites for vesicle fusion. The utilization of release sites is regulated to determine spatiotemporal organization of the two main forms of synchronous release, uni-vesicluar (UVR) and multi-vesicular (MVR). We previously found that the vesicle-associated molecular motor myosin V regulates temporal utilization of release sites by controlling vesicle anchoring at release sites (Maschi et al, 2018). Here we show that acute inhibition of myosin V shifts preferential location of vesicle docking away from AZ center towards periphery, and results in a corresponding spatial shift in utilization of release sites during UVR. Similarly, inhibition of myosin V also reduces preferential utilization of central release sites during MVR, leading to more spatially distributed and temporally uniform MVR that occurs farther away from the AZ center. Thus myosin V regulates both temporal and spatial utilization of release sites during two main forms of synchronous release.

Testosterone induction of poly(A)+-RNA synthesis and [35S]methionine incorporation into proteins of Rana esculenta Harderian gland

1992 ◽  
Vol 84 (3) ◽  
pp. R51-R56 ◽  
Author(s):  
B. Varriale ◽  
G. Chieffi-Baccari ◽  
M. d'Istria ◽  
L.Di Matteo ◽  
S. Minucci ◽  
...  
Keyword(s):  
Rna Synthesis ◽  
Harderian Gland ◽  
Rana Esculenta

Effect of cholinergic secretagogue substances on the morphology of the Harderian gland in the frog, Rana esculenta

1995 ◽  
Vol 112 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Loredana Di Matteo ◽  
Gabriella Chieffi Baccari ◽  
Sergio Minucci
Keyword(s):  
Harderian Gland ◽  
Rana Esculenta
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