Metabolomic Profiles of the Creeping Wood Sorrel Oxalis corniculata in Radioactively Contaminated Fields in Fukushima: Dose-Dependent Changes in Key Metabolites

The biological impacts of the Fukushima nuclear accident, in 2011, on wildlife have been studied in many organisms, including the pale grass blue butterfly and its host plant, the creeping wood sorrel Oxalis corniculata. Here, we performed an LC–MS-based metabolomic analysis on leaves of this plant collected in 2018 from radioactively contaminated and control localities in Fukushima, Miyagi, and Niigata prefectures, Japan. Using 7967 peaks detected by LC–MS analysis, clustering analyses showed that nine Fukushima samples and one Miyagi sample were clustered together, irrespective of radiation dose, while two Fukushima (Iitate) and two Niigata samples were not in this cluster. However, 93 peaks were significantly different (FDR < 0.05) among the three dose-dependent groups based on background, low, and high radiation dose rates. Among them, seven upregulated and 15 downregulated peaks had single annotations, and their peak intensity values were positively and negatively correlated with ground radiation dose rates, respectively. Upregulated peaks were annotated as kudinoside D (saponin), andrachcinidine (alkaloid), pyridoxal phosphate (stress-related activated vitamin B6), and four microbe-related bioactive compounds, including antibiotics. Additionally, two peaks were singularly annotated and significantly upregulated (K1R1H1; peptide) or downregulated (DHAP(10:0); decanoyl dihydroxyacetone phosphate) most at the low dose rates. Therefore, this plant likely responded to radioactive pollution in Fukushima by upregulating and downregulating key metabolites. Furthermore, plant-associated endophytic microbes may also have responded to pollution, suggesting their contributions to the stress response of the plant.

On the importance of the electric double layer structure in aqueous electrocatalysis

To design electrochemical interfaces for efficient electric-chemical energy interconversion, it is critical to reveal the electric double layer (EDL) structure and relate it with electrochemical activity; nonetheless, this has been a long-standing challenge. Of particular, no molecular-level theories have fully explained the characteristic two peaks arising in the potential-dependence of the EDL capacitance, which is sensitively dependent on the EDL structure. We herein demonstrate that our first-principles-based molecular simulation reproduces the experimental capacitance peaks. The origin of two peaks emerging at anodic and cathodic potentials is unveiled to be an electrosorption of ions and a structural phase transition, respectively. We further find a cation complexation gradually modifies the EDL structure and the field strength, which linearly scales the carbon dioxide reduction activity. This study deciphers the complex structural response of the EDL and highlights its catalytic importance, which bridges the mechanistic gap between the EDL structure and electrocatalysis.

Immigration dynamics of tropical and subtropical Southeast Asian limestone karst floras

Ex situ origins and dispersal of taxa have played important roles in the assembly of island-like biodiversity hotspots. Insular limestone karsts in Southeast Asia are hotspots of biodiversity and endemism, but the immigration processes of their unique floras are still poorly known. Here, we used Gesneriaceae as a proxy to investigate the immigration dynamics of tropical and subtropical Southeast Asian karst floras. We present the most comprehensive phylogenetic analysis of the Old World gesneriads to date based on twelve loci. By estimating divergence times and reconstructing ancestral states (habitat, soil type and range), we found that immigration into subtropical Southeast Asian karst floras first occurred in the Early Miocene, with two peaks in the Early–Middle Miocene and the Pliocene–Early Pleistocene, whereas immigration into tropical Southeast Asian karsts initiated in the Late Eocene, with two peaks in the Late Oligocene and the Late Miocene. We also discover that Southeast Asian karst biodiversity comprises immigrant pre-adapted lineages and descendants from local acid soil ancestors, although niche shift from acid soil to karst in tropical Southeast Asian islands was lacking. This study advances our understanding of the historical assembly of Southeast Asian karst floras.

Research on performance test analysis method of mechanical structure based on measured error / virtual simulation

Mechanical errors seriously affect the transmission performance of mechanical structure, especially for high-precision mechanical structure. With the development of virtual simulation technology, the performance test of mechanical structure in virtual environment is gradually applied. Under the background, this paper takes the movable tooth reducer as the research object and puts forward a virtual performance test method by combing test errors with virtual simulation technology. The tooth profile errors of key components of the reducer are obtained by optical scanning, and the simulation test is carried out. Meanwhile, the accuracy of the simulation test is verified by the implementation of vibration test. The results show that both the simulated vibration acceleration and the experimental test vibration acceleration show a double-peak variation trend, and the frequency difference between the two peaks is less than 5%. Therefore, the simulation test method proposed in this paper can effectively evaluate the performance of mechanical structures.

Бимодальность в спектрах электролюминесценции InGaAs квантовых яма-точек

The electroluminescence spectra of waveguiding structures based on quantum well-dots were investigated with polarization resolution in the temperature range of 60−300 K. It is found that the ground state emission consists of two peaks with different degrees of TE-polarization and these peaks are getting closer with temperature decrease. We attribute the bimodality to the existence of two different types of nanoobjects in the active region: the quantum well-dots, which have partially TE-polarized emission, and quantum dots emitting almost fully TE-polarized light.

Heterologous Overexpression of Sup35 in E. coli Leads to Both Monomer and Complex States

The heterologous overexpression states of prion proteins play a critical role in understanding the mechanisms of prion-related diseases. We report herein the identification of soluble monomer and complex states for a bakers’ yeast prion, Sup35, when expressed in E. coli. Two peaks are apparent with the elution of His-tagged Sup35 by imidazole from a Ni affinity column. Peak I contains Sup35 in both monomer and aggregated states. Sup35 aggregate is abbreviated as C-aggregate and includes a non-fibril complex comprising Sup35 aggregate-HSP90-Dna K, ATP synthase β unit (chain D), 30S ribosome subunit, and Omp F. The purified monomer and C-aggregate can remain stable for an extended period of time. Peak II contains Sup35 also in both monomer and aggregated (abbreviated as S-aggregate) states, but the aggregated states are caused by the formation of inter-Sup35 disulfide bonds. This study demonstrates that further assembly of Sup35 non-fibril C-aggregate can be interrupted by the chaperone repertoire system in E. coli.

Phenological and social characterization of three Lasioglossum (Dialictus) species inferred from long-term trapping collections

Detailed social and phenological data collected from nesting aggregations exist for relatively few sweat bee species because nesting aggregations are rarely found in large numbers, even when local populations are highly abundant. This limits researchers’ abilities to assess the social status of many species, which in turn, limits our ability to trace the sequence of evolutionary steps between alternative social states. To address this problem, we demonstrate the utility of rehydrated, pinned specimens from pan trap and netting collections for generating inferences about the phenology and social status of a well-studied sweat bee species, Lasioglossum (Dialictus) laevissimum. A detailed comparison of phenology and reproductive traits, between pinned specimens and those in a previous nesting study, produced similar results for bivoltine foraging activity and eusocial colony organization typical in this species. We then used pinned specimens from monitoring studies to describe, for the first time, the foraging phenology and social behaviour of two additional Dialictus species, L. hitchensi and L. ellisiae. Both L. hitchensi and L. ellisiae each exhibited two peaks in abundance during their breeding seasons, indicating two periods of foraging activity, which correspond to provisioning of spring and summer broods. Differences in body size, wear, and ovarian development of spring and summer females indicated that L. hitchensi is most likely eusocial, while L. ellisiae is either solitary or communal. This study demonstrates that analyses of specimens obtained from flower and pan trap collections can be used for assessing the phenology and social organization of temperate sweat bees in the absence of nesting data. The phenological and social lability of many sweat bee species make them ideal for studying geographic and temporal variability in sociality, and analyses of pan trap collections can make these studies possible when direct observations are impossible.

Investigation of Partial Shading Scenarios on a Photovoltaic Array’s Characteristics

The purpose of this study is to investigate the impact of different partial shading scenarios on a PV array’s characteristics in order to develop a simple and easy-to-implement GMPP controller that tracks the PV array’s global maximum power point (GMPP). The P-V characteristic of the PV array becomes more complicated under partial shading, owing to the presence of many power peaks, as opposed to uniform irradiance conditions, when there is only one peak called the maximum power point. In fact, and according to an experiment conducted in this study, when a PV array is partially shaded, the P-V characteristic mostly presents two peaks, given the existence of only two levels of irradiance, one of which is called the global peak (i.e., the GMPP). Furthermore, the first peak is located at Vmpp1 (the PV array’s voltage corresponds to this peak), whereas the second is at Vmpp2. The proposed approach works by estimating the values of Vmpp1 and Vmpp2 using two equations in order to control the DC/DC converter of the PV system. The first equation is used when the GMPP is at the first peak, while the other is used when the GMPP is at the second peak. Several scenarios are simulated and presented in this paper to verify the accuracy of these equations. In addition, some conclusions are drawn to suggest a simple method for tracking the GMPP.

Effect of microencapsulated chavil (Ferulago angulata) extract on physicochemical, microbiological, textural and sensorial properties of UF-feta-type cheese during storage time

Chavil (Ferulago angulata) extract (CE) and microencapsulated chavil extract (MCE) were added to UF- Feta-type Cheese. The aim of this study was to comprising CE and MCE on physicochemical and microbiological properties in cheese. The scanning electron microscope images demonstrate the MCE had elliptical shape. The average size diameter curve of the MCE revealed bimodal distribution with two peaks (1541 and 2222 nm) separately. The hardness value of MCE cheese (212.83 ± 17.63 g) was lower than that of CE (343.67 ± 25.53 g) because of canola oil used in the microencapsulation process. The MCE-cheese showed lower values of acidity (1.67%) in comparison with CE-cheese (1.87%). The viable numbers of Streptococcus thermophilus and Lactococcus lactis were equal among the samples (4.6–4.9 log10 CFU/g respectively). The acid degree value of MCE (2.07 ± 0.21%) and CE (1.83 ± 0.25%) cheese were nearly equal at the end of storage time.

Tailoring Magnetic and Transport Anisotropies in Co100−x–Cux Thin Films through Obliquely Grown Nano-Sheets

The magnetic and transport properties of pulsed laser-deposited Co100−x–Cux thin films were tailored through their nano-morphology and composition by controlling for the deposition geometry, namely normal or oblique deposition, and their Cu content. All films were composed of an amorphous Co matrix and a textured growth of Cu nanocrystals, whose presence and size d increased as x increased. For x = 50, all films were superparamagnetic, regardless of deposition geometry. The normally deposited films showed no in-plane magnetic anisotropy. On the contrary, controllable in-plane uniaxial magnetic anisotropy in both direction and magnitude was generated in the obliquely deposited films. The magnetic anisotropy field Hk remained constant for x = 0, 5 and 10, Hk ≈ 35 kAm−1, and decreased to 28 and 26 kAm−1 for x = 20 and 30, respectively. This anisotropy had a magnetostatic origin due to a tilted nano-sheet morphology. In the normally deposited films, the coercive field Hc increased when x increased, from 200 (x = 0) to 1100 Am−1 (x = 30). In contrast, in obliquely deposited films, Hc decreased from 1500 (x = 0) to 100 Am−1 (x = 30) as x increased. Activation energy spectra corresponding to structural relaxation phenomena in obliquely deposited films were obtained from transport property measurements. They revealed two peaks, which also depended on their nano-morphology and composition.