Exocytosis of neurosecretory granules from the crustacean sinus gland in freeze-fracture

1976 ◽  
Vol 150 (1) ◽  
pp. 227-252 ◽  
Author(s):  
Richard R. Shivers
Author(s):  
Richard R. Shivers

The sinus gland is a neurohemal organ located in the crayfish eyestalk and represents a storage site for neurohormones prior to their release into the circulation. The sinus gland contains 3 classes of dense, membrane-limited granules: 1) granules measuring less than 1000 Å in diameter, 2) granules measuring 1100-1400 Å in diameter, and 3) granules measuring 1500-2000 Å in diameter. Class 3 granules are the most electron-dense of the granules found in the sinus gland, while class 2 granules are the most abundant. Generally, all granules appear to undergo similar changes during release.Release of neurosecretory granules may be initiated by a preliminary fragmentation of the “parent granule” into smaller, less dense vesicles which measure about 350 Å in diameter (V, Figs. 1-3). A decrease in density of the granules prior to their fragmentation has been observed and may reflect a change in the chemical nature of the granule contents.


Author(s):  
Ann Heffington Bunt ◽  
Ebert A. Ashby

The sinus gland of the crayfish, Procambarus clarkii, is located on the dorsum of the eyestalk, just beneath the exoskeleton and adjacent to the medullae interna and externa optic ganglia. It functions to secrete a variety of proteinaceous hormones, including the erythrophore concentrating hormone, melanophore dispersing hormone, molt inhibiting hormone, diabetogenic hormone, distal retinal pigment hormone, and ovary inhibiting hormone.The gland is composed of numerous neurosecretory axon terminals clustered about a branching blood sinus. The neurosecretory axons arise from cells lying some distance away from the sinus gland, in the medulla terminal is X-organ, the brain, and possibly the thoracic ganglion. The hormones are manufactured in the perikarya of these cells and transported through the axons to their terminals in the sinus gland for storage and release into the blood sinus.Small, electron dense spherules within the axons contain the hormone secretory product. These neurosecretory granules are very similar in morphology to those reported in the sinus glands of the dwarf crayfish, Cambarellus shufeldti, the land crab, Gecarcinus lateralis, and the Mediterranean isopod, Sguilla mantis. The sinus glands of each of these crustaceans contain two size ranges of neurosecretory granules: 1500-2000A and 500-900A.


1997 ◽  
Vol 17 (1) ◽  
pp. 6 ◽  
Author(s):  
Jocelyne Castany ◽  
François Van Herp ◽  
Guy Charmantier ◽  
Jean-Paul Trilles ◽  
Mireille Charmantier-Daures ◽  
...  

Author(s):  
P.L. Moore

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.


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