Depletion of neurosecretory granules and membrane retrieval in the sinus gland of the crab

1980 ◽  
Vol 205 (1) ◽  
Author(s):  
JeanJ. Nordmann ◽  
JohnF. Morris
Author(s):  
Richard R. Shivers

The sinus gland is a neurohemal organ located in the crayfish eyestalk and represents a storage site for neurohormones prior to their release into the circulation. The sinus gland contains 3 classes of dense, membrane-limited granules: 1) granules measuring less than 1000 Å in diameter, 2) granules measuring 1100-1400 Å in diameter, and 3) granules measuring 1500-2000 Å in diameter. Class 3 granules are the most electron-dense of the granules found in the sinus gland, while class 2 granules are the most abundant. Generally, all granules appear to undergo similar changes during release.Release of neurosecretory granules may be initiated by a preliminary fragmentation of the “parent granule” into smaller, less dense vesicles which measure about 350 Å in diameter (V, Figs. 1-3). A decrease in density of the granules prior to their fragmentation has been observed and may reflect a change in the chemical nature of the granule contents.


Author(s):  
Ann Heffington Bunt ◽  
Ebert A. Ashby

The sinus gland of the crayfish, Procambarus clarkii, is located on the dorsum of the eyestalk, just beneath the exoskeleton and adjacent to the medullae interna and externa optic ganglia. It functions to secrete a variety of proteinaceous hormones, including the erythrophore concentrating hormone, melanophore dispersing hormone, molt inhibiting hormone, diabetogenic hormone, distal retinal pigment hormone, and ovary inhibiting hormone.The gland is composed of numerous neurosecretory axon terminals clustered about a branching blood sinus. The neurosecretory axons arise from cells lying some distance away from the sinus gland, in the medulla terminal is X-organ, the brain, and possibly the thoracic ganglion. The hormones are manufactured in the perikarya of these cells and transported through the axons to their terminals in the sinus gland for storage and release into the blood sinus.Small, electron dense spherules within the axons contain the hormone secretory product. These neurosecretory granules are very similar in morphology to those reported in the sinus glands of the dwarf crayfish, Cambarellus shufeldti, the land crab, Gecarcinus lateralis, and the Mediterranean isopod, Sguilla mantis. The sinus glands of each of these crustaceans contain two size ranges of neurosecretory granules: 1500-2000A and 500-900A.


1997 ◽  
Vol 17 (1) ◽  
pp. 6 ◽  
Author(s):  
Jocelyne Castany ◽  
François Van Herp ◽  
Guy Charmantier ◽  
Jean-Paul Trilles ◽  
Mireille Charmantier-Daures ◽  
...  

Author(s):  
Ernest F. Couch

Crustacea, like insects, possess ecdysial glands that produce a hormone governing molting and growth. The hormone is a steriod and the same steroid has been found in insects. The steriod has been given several names, among them are crustecdysone and ecdysterone. The ecdysial glands in insects are named prothoracic glands while the homologous structures in crayfish and other Crustacea are called Y-organs. Y-organs do not always contain the same titer of ecdysterone (Passano and Jyssum, 1963), having the greatest amount of the hormone during the period preceding ecdysis. The cyclical activity of the gland is controlled by a neurosecretory hormone, the molt inhibiting hormone (MIH), released by the sinus gland.Y-organs for this study were obtained from the crayfish, Procambarus simulans. They were fixed with either 3% glutaraldehyde followed by 1% osmium tetroside or simply fixed in buffered 1% osmium. Tissues were embedded in Araldite 502 and prepared for electron miscroscopy. Glands were taken from animals in various periods of the molt cycle.


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