Purification and Characterization of the Active-Site-Mutated Recombinant Human μ-Calpain Expressed in Baculovirus-Infected Insect Cells

1998 ◽  
Vol 246 (3) ◽  
pp. 681-685 ◽  
Author(s):  
Kiyotaka Hitomi ◽  
Yasutoshi Uchiyama ◽  
Iwao Ohkubo ◽  
Mitoshi Kunimatsu ◽  
Makoto Sasaki ◽  
...  
Keyword(s):  
Active Site ◽  
Insect Cells ◽  
Purification And Characterization ◽  
Infected Insect

Purification and characterization of truncated human AMPK α2β2γ3 heterotrimer from baculovirus-infected insect cells

2010 ◽  
Vol 70 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Lata Ramanathan ◽  
Payal R. Sheth ◽  
Polina Ogas ◽  
Li Xiao ◽  
Hung V. Le
Keyword(s):  
Insect Cells ◽  
Purification And Characterization ◽  
Infected Insect

scholarly journals Expression of stable human O-glycan core 2 β-1,6-N-acetylglucosaminyltransferase in Sf9 insect cells

1997 ◽  
Vol 325 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Dale TOKI ◽  
Mohan SARKAR ◽  
Betty YIP ◽  
Folkert RECK ◽  
David JOZIASSE ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Culture Medium ◽  
Insect Cells ◽  
Cell System ◽  
Purification And Characterization ◽  
Truncated Form ◽  
Natural Sources ◽  
Glycosylation Sites ◽  
Infected Insect

UDP-GlcNAc:Galβ1-3GalNAc-R (GlcNAc to GalNAc) β-1,6-N-acetylglucosaminyltransferase (C2GnT) catalyses the formation of O-glycan core 2. Purification and characterization of C2GnT from natural sources has been hampered by the instability of this enzyme. We have been able to prepare a stable partly purified recombinant human C2GnT by expression of a truncated form of the enzyme in the baculovirus/Spodoptera frugiperda 9 (Sf9) insect cell system. C2GnT activity was secreted into the Sf9 culture medium (15 pmol/min per μl; approx. 0.2 mg/l) and was stable at 4 °C either in solution or after lyophilization. Endoglycosidase H and N-glycanase F treatment of the radiolabelled C2GnT indicated the presence of N-glycans at both potential N-glycosylation sites. The elimination of one or both of the two potential N-glycosylation sites or treatment of the virus-infected insect cells with tunicamycin resulted in loss of enzyme activity due in part to protein degradation.

Purification and characterization of secreted human leptin produced in baculovirus-infected insect cells

Gene ◽  
1997 ◽  
Vol 190 (1) ◽  
pp. 131-137 ◽  
Author(s):  
Lisa M. Churgay ◽  
Steven Kovacevic ◽  
Frank C. Tinsley ◽  
Cheryl M. Kussow ◽  
Rohn L. Millican ◽  
...  
Keyword(s):  
Insect Cells ◽  
Purification And Characterization ◽  
Infected Insect
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