Letter to the editor: Squalene is Localized to the Plasma Membrane in Bovine Retinal Rod Outer Segments

1997 ◽  
Vol 64 (2) ◽  
pp. 279-282 ◽  
Author(s):  
STEVEN J. FLIESLER ◽  
KATHLEEN BOESZE-BATTAGLIA ◽  
ZOPHIA PAW ◽  
R.KENNEDY KELLER ◽  
ARLENE D. ALBERT
Keyword(s):  
Plasma Membrane ◽  
Rod Outer Segments ◽  
Outer Segments ◽  
Letter To The Editor ◽  
Retinal Rod ◽  
Retinal Rod Outer Segments

Ca2+ influx into bovine retinal rod outer segments mediated by Na+/Ca2+/K+ exchange

1995 ◽  
Vol 269 (5) ◽  
pp. C1153-C1159 ◽  
Author(s):  
P. P. Schnetkamp ◽  
J. E. Tucker ◽  
R. T. Szerencsei
Keyword(s):  
Plasma Membrane ◽  
Light Adaptation ◽  
Rod Outer Segments ◽  
Site Model ◽  
Outer Segments ◽  
Retinal Rod ◽  
Absolute Requirement ◽  
Previous Loading ◽  
Retinal Rod Outer Segments ◽  
Ionophore Monensin

Ca(2+)-depleted rod outer segments (ROS) were purified from bovine retinal rod photoreceptors, and factors influencing Ca2+ influx into ROS via the plasma membrane Na+/Ca2+/K+ exchanger were analyzed. Intracellular alkali cation concentrations were manipulated by 1) previous loading via the ionophore monensin followed by removal of monensin and 2) addition of the channel ionophore gramicidin during Ca(2+)-influx measurements. Ca2+ influx was measured as a rise in cytosolic free Ca2+ with the Ca(2+)-indicating dye fluo 3. An absolute requirement for intracellular Na+ was observed with a Na+ dissociation constant of 30-40 mM, whereas intracellular K+ was a potent inhibitor of Ca2+ influx, apparently by competing with Na+ for a common site on the Na+/Ca2+/K+ exchanger. Half-maximal Ca2+ influx was observed at an external free Ca2+ concentration of 0.9 microM when no external Na+ was present and 3.5 microM when 10 mM external Na+ was present. Our observations are discussed in the context of 1) a three-site model for the Na+/Ca2+/K+ exchanger and 2) earlier experiments on light adaptation in rods, which depended on minimizing Ca2+ fluxes across the ROS plasma membrane.

scholarly journals Mg2+ -ATP induces filament growth from retinal rod outer segments with disrupted plasma membranes

FEBS Letters ◽  
1987 ◽  
Vol 211 (1) ◽  
pp. 35-40 ◽  
Author(s):  
K.R. Parker ◽  
L.E. Schaechter ◽  
J.W. Lewis ◽  
K.L. Zeman ◽  
D.S. Kliger ◽  
...  
Keyword(s):  
Plasma Membranes ◽  
Rod Outer Segments ◽  
Outer Segments ◽  
Retinal Rod ◽  
Retinal Rod Outer Segments

Patterns of Experimental Allergic Uveitis Induced by Rhodopsin and Retinal Rod Outer Segments

1980 ◽  
Vol 12 (3) ◽  
pp. 165-176 ◽  
Author(s):  
George E. Marak, Jr ◽  
Hitoshi Shichi ◽  
Narsing A. Rao ◽  
Waldon B. Wacker
Keyword(s):  
Rod Outer Segments ◽  
Outer Segments ◽  
Retinal Rod ◽  
Retinal Rod Outer Segments ◽  
Experimental Allergic

Effect of tocopherol and taurine on membrane fluidity of retinal rod outer segments

1987 ◽  
Vol 45 (6) ◽  
pp. 769-776 ◽  
Author(s):  
Julio Moran ◽  
Patricia Salazar ◽  
Herminia Pasantes-Morales
Keyword(s):  
Membrane Fluidity ◽  
Rod Outer Segments ◽  
Outer Segments ◽  
Retinal Rod ◽  
Retinal Rod Outer Segments

Purification of a complex of transducin βγ subunits from isotonic extracts of bovine retinal rod outer segments

BIOPHYSICS ◽  
2010 ◽  
Vol 55 (6) ◽  
pp. 908-910 ◽  
Author(s):  
D. N. Orlov ◽  
T. G. Orlova ◽  
A. R. Nezvetsky ◽  
N. Ya. Orlov
Keyword(s):  
Rod Outer Segments ◽  
Outer Segments ◽  
Retinal Rod ◽  
Retinal Rod Outer Segments
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