Isolation, Electron Microscopic Imaging, and 3-D Visualization of Native Cardiac Thin Myofilaments

1999 ◽  
Vol 126 (2) ◽  
pp. 98-104 ◽  
Author(s):  
Martin Spiess ◽  
Michel O Steinmetz ◽  
Anna Mandinova ◽  
Bettina Wolpensinger ◽  
Ueli Aebi ◽  
...  
Keyword(s):  
Electron Microscopic ◽  
Microscopic Imaging

scholarly journals DSS1 and ssDNA regulate oligomerization of BRCA2

2020 ◽  
Vol 48 (14) ◽  
pp. 7818-7833 ◽  
Author(s):  
Hang Phuong Le ◽  
Xiaoyan Ma ◽  
Jorge Vaquero ◽  
Megan Brinkmeyer ◽  
Fei Guo ◽  
...  
Keyword(s):  
Replication Fork ◽  
Microscopic Analysis ◽  
Filament Formation ◽  
Electron Microscopic ◽  
Microscopic Imaging ◽  
Cellular Events ◽  
Self Association ◽  
Oligomeric Forms

Abstract The tumor suppressor BRCA2 plays a key role in initiating homologous recombination by facilitating RAD51 filament formation on single-stranded DNA. The small acidic protein DSS1 is a crucial partner to BRCA2 in this process. In vitro and in cells (1,2), BRCA2 associates into oligomeric complexes besides also existing as monomers. A dimeric structure was further characterized by electron microscopic analysis (3), but the functional significance of the different BRCA2 assemblies remains to be determined. Here, we used biochemistry and electron microscopic imaging to demonstrate that the multimerization of BRCA2 is counteracted by DSS1 and ssDNA. When validating the findings, we identified three self-interacting regions and two types of self-association, the N-to-C terminal and the N-to-N terminal interactions. The N-to-C terminal self-interaction of BRCA2 is sensitive to DSS1 and ssDNA. The N-to-N terminal self-interaction is modulated by ssDNA. Our results define a novel role of DSS1 to regulate BRCA2 in an RPA-independent fashion. Since DSS1 is required for BRCA2 function in recombination, we speculate that the monomeric and oligomeric forms of BRCA2 might be active for different cellular events in recombinational DNA repair and replication fork stabilization.

Vectorial sequence of mineralization in the turkey leg tendon determined by electron microscopic imaging

1991 ◽  
Vol 48 (1) ◽  
pp. 46-55 ◽  
Author(s):  
A. Larry Arsenault ◽  
Brad W. Frankland ◽  
F. Peter Ottensmeyer
Keyword(s):  
Electron Microscopic ◽  
Microscopic Imaging ◽  
Turkey Leg Tendon

Electron microscopic imaging and contrast of smallest carbon nanotubes

2001 ◽  
Vol 349 (5-6) ◽  
pp. 389-393 ◽  
Author(s):  
Lu-Chang Qin ◽  
Xinluo Zhao ◽  
Kaori Hirahara ◽  
Yoshinori Ando ◽  
Sumio Iijima
Keyword(s):  
Carbon Nanotubes ◽  
Electron Microscopic ◽  
Microscopic Imaging
Sign in / Sign up

Export Citation Format

Share Document