Phylogenetic Relationships of Chelid Turtles (Pleurodira: Chelidae) Based on Mitochondrial 12S rRNA Gene Sequence Variation

1997 ◽  
Vol 7 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Jennifer M. Seddon ◽  
Arthur Georges ◽  
Peter R. Baverstock ◽  
William McCord
Keyword(s):  
Phylogenetic Relationships ◽  
Sequence Variation ◽  
Gene Sequence ◽  
12S Rrna ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

Analysis of mitochondrial 12S rRNA gene sequence variation in four ethnically defined populations

2003 ◽  
Vol 1239 ◽  
pp. 75-78
Author(s):  
C Albarrán ◽  
P Martı́n ◽  
O Garcı́a ◽  
P Garcı́a ◽  
L Fernández de Simón ◽  
...  
Keyword(s):  
Sequence Variation ◽  
Gene Sequence ◽  
12S Rrna ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

scholarly journals Sequence Characterization of Mitochondrial 12S rRNA Gene in Mouse Deer (Moschiola indica) for PCR-RFLP Based Species Identification

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Chandra Mohan Siddappa ◽  
Mohini Saini ◽  
Asit Das ◽  
Ramesh Doreswamy ◽  
Anil K. Sharma ◽  
...  
Keyword(s):  
Species Identification ◽  
Genomic Dna ◽  
Gene Sequence ◽  
12S Rrna ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Deer Species ◽  
12S Rrna Gene ◽  
Pcr Rflp ◽  
Mitochondrial 12S Rrna

Mitochondrial 12S rRNA has proven to be a useful molecular marker for better conservation and management of the endangered species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the mitochondrial 12S rRNA gene has proven to be a reliable and efficient tool for the identification of different Indian deer species of family cervidae. In the present study, mitochondrial 12S rRNA gene sequence of mouse deer (Moschiola indica) belonging to the family Tragulidae was characterized and analysed in silico for its use in species identification. Genomic DNA was isolated from the hair follicles and mitochondrial 12S rRNA gene was amplified using universal primers. PCR product was cloned and sequenced for the first time. The sequence of mouse deer showed 90.04, 90.08, 90.04, 91.2, 90.04, and 90.08% identities with sika deer, sambar, hog deer, musk deer, chital, and barking deer, respectively. Restriction mapping in Lasergene (DNAstar Inc., Madison, WI, USA) revealed that mouse deer mitochondrial 12S rRNA gene sequence can be differentiated from the other deer species in PCR-RFLP using RsaI, DdeI, BsrI, and BstSFI. With the help of predicted pattern, mouse deer can be identified using genomic DNA from a variety of biomaterials, thereby providing molecular aid in wildlife forensics and conservation of the species.

Phylogenetic Relationships among the Species of the Genus Testudo (Testudines: Testudinidae) Inferred from Mitochondrial 12S rRNA Gene Sequences

2002 ◽  
Vol 22 (2) ◽  
pp. 174-183 ◽  
Author(s):  
Antoinette C. van der Kuyl ◽  
Donato L. Ph. Ballasina ◽  
John T. Dekker ◽  
Jolanda Maas ◽  
Ronald E. Willemsen ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
12S Rrna ◽  
Rrna Gene ◽  
Gene Sequences ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

scholarly journals Phylogenetic relationships of snails of the genera Oncomelania and Tricula inferred from the mitochondrial 12S rRNA gene

2003 ◽  
Vol 31 (1) ◽  
pp. 5-10 ◽  
Author(s):  
MUNEHIRO OKAMOTO ◽  
CHIN-TSON LO ◽  
WILFRED U. TIU ◽  
DONGCHUAN QUI ◽  
PINARDI HADIDJAJA ◽  
...  
Keyword(s):  
Phylogenetic Relationships ◽  
12S Rrna ◽  
Rrna Gene ◽  
12S Rrna Gene ◽  
Mitochondrial 12S Rrna

Assessing the phylogenetic signal of the nuclear β-Fibrinogen intron 7 in salamandrids (Amphibia: Salamandridae)

2006 ◽  
Vol 27 (3) ◽  
pp. 409-418 ◽  
Author(s):  
Fernando Sequeira ◽  
Nuno Ferrand ◽  
D. James Harris
Keyword(s):  
Phylogenetic Relationships ◽  
Phylogenetic Signal ◽  
12S Rrna ◽  
Rrna Gene ◽  
Length Variation ◽  
Β Subunit ◽  
Phylogenetic Studies ◽  
12S Rrna Gene ◽  
Considerable Length ◽  
Mitochondrial 12S Rrna

AbstractThe potential of the seventh intron of the β-subunit of the fibrinogen gene (β-fibint 7) for phylogenetic analysis within the Salamandridae family was explored, comparing the topologies of trees based on this marker to those based on mitochondrial 12S rRNA gene previously published. Using primers designed specifically for amphibians, we amplified 25 sequences of β-fibint 7 corresponding to 15 species of salamandrids and one plethodontid species. There was considerable length variation among the β-fibint 7 sequences examined, ranging from 1123 bp in S. atra to 400 bp in P. waltl. Many aspects of the phylogenetic relationships estimated by the two independent loci were congruent and corroborate current taxonomic hypothesis. Although the number of taxa analysed is small, the data obtained in this work suggested that β-fibint 7 is a useful marker for assessing phylogenetic relationships within the Salamandridae family, and is probably appropriate for phylogenetic studies among closely related salamanders that have diverged over the last 20 Myr.

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