scholarly journals Identification and Characterization of a 65-kDa Protein Processed from the Gene 1 Polyprotein of the Murine Coronavirus MHV-A59

Virology ◽  
1995 ◽  
Vol 207 (1) ◽  
pp. 316-320 ◽  
Author(s):  
Mark R. Denison ◽  
Scott A. Hughes ◽  
Susan R. Weiss
Keyword(s):  
Identification And Characterization ◽  
Murine Coronavirus

scholarly journals Identification and characterization of a serine-like proteinase of the murine coronavirus MHV-A59.

1995 ◽  
Vol 69 (6) ◽  
pp. 3554-3559 ◽  
Author(s):  
Y Lu ◽  
X Lu ◽  
M R Denison
Keyword(s):  
Identification And Characterization ◽  
Murine Coronavirus

scholarly journals Identification and Characterization of a Proteolytically Primed Form of the Murine Coronavirus Spike Proteins after Fusion with the Target Cell

2014 ◽  
Vol 88 (9) ◽  
pp. 4943-4952 ◽  
Author(s):  
O. Wicht ◽  
C. Burkard ◽  
C. A. M. de Haan ◽  
F. J. M. van Kuppeveld ◽  
P. J. M. Rottier ◽  
...  
Keyword(s):  
Target Cell ◽  
Identification And Characterization ◽  
Murine Coronavirus ◽  
Spike Proteins

scholarly journals Identification of the Murine Coronavirus MP1 Cleavage Site Recognized by Papain-Like Proteinase 2

2003 ◽  
Vol 77 (13) ◽  
pp. 7376-7382 ◽  
Author(s):  
Amornrat Kanjanahaluethai ◽  
Dalia Jukneliene ◽  
Susan C. Baker
Keyword(s):  
Cleavage Site ◽  
Edman Degradation ◽  
Putative Cleavage Site ◽  
Amino Terminal ◽  
Cleavage Assay ◽  
Degradation Reaction ◽  
Methionine Residues ◽  
Identification And Characterization ◽  
Murine Coronavirus

ABSTRACT The replicase polyprotein of murine coronavirus is extensively processed by three proteinases, two papain-like proteinases (PLPs), termed PLP1 and PLP2, and a picornavirus 3C-like proteinase (3CLpro). Previously, we established a trans-cleavage assay and showed that PLP2 cleaves the replicase polyprotein between p210 and membrane protein 1 (MP1) (A. Kanjanahaluethai and S. C. Baker, J. Virol. 74:7911-7921, 2000). Here, we report the results of our studies identifying and characterizing this cleavage site. To determine the approximate position of the cleavage site, we expressed constructs that extended various distances upstream from the previously defined C-terminal end of MP1. We found that the construct extending from the putative PLP2 cleavage site at glycine 2840-alanine 2841 was most similar in size to the processed MP1 replicase product generated in a trans-cleavage assay. To determine which amino acids are critical for PLP2 recognition and processing, we generated 14 constructs with amino acid substitutions upstream and downstream of the putative cleavage site and assessed the effects of the mutations in the PLP2 trans-cleavage assay. We found that substitutions at phenylalanine 2835, glycine 2839, or glycine 2840 resulted in a reduction in cleavage of MP1. Finally, to unequivocally identify this cleavage site, we isolated radiolabeled MP1 protein and determined the position of [35S]methionine residues released by Edman degradation reaction. We found that the amino-terminal residue of MP1 corresponds to alanine 2841. Therefore, murine coronavirus PLP2 cleaves the replicase polyprotein between glycine 2840 and alanine 2841, and the critical determinants for PLP2 recognition and processing occupy the P6, P2, and P1 positions of the cleavage site. This study is the first report of the identification and characterization of a cleavage site recognized by murine coronavirus PLP2 activity.

Identification and characterization of peptidases secreted by quahog parasite unknown (QPX), the protistan parasite of hard clams

2016 ◽  
Vol 122 (1) ◽  
pp. 21-33 ◽  
Author(s):  
E Rubin ◽  
GT Werneburg ◽  
E Pales Espinosa ◽  
DG Thanassi ◽  
B Allam
Keyword(s):  
Quahog Parasite Unknown ◽  
Hard Clams ◽  
Identification And Characterization

Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor

2013 ◽  
Vol 103 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Q Jiang ◽  
L Shi ◽  
C Ke ◽  
W You ◽  
J Zhao
Keyword(s):  
Vibrio Harveyi ◽  
Haliotis Diversicolor ◽  
Identification And Characterization

IDENTIFICATION AND CHARACTERIZATION OF 1,2-BN CYCLOHEXENE USING MICROWAVE SPECTROSCOPY

2016 ◽  
Author(s):  
Stephen Kukolich ◽  
Shih-Yuan Liu ◽  
Jacob Ishibashi ◽  
Adam Daly ◽  
Ming Sun
Keyword(s):  
Microwave Spectroscopy ◽  
Identification And Characterization

Identification and characterization of a CRM1/XPO1-dependent nuclear export signal in the human androgen receptor

2016 ◽  
Author(s):  
Stefanie V Schutz ◽  
Axel Merseburger ◽  
Anca Azoitei ◽  
Marcus V Cronauer
Keyword(s):  
Androgen Receptor ◽  
Nuclear Export ◽  
Nuclear Export Signal ◽  
Export Signal ◽  
Identification And Characterization ◽  
Human Androgen Receptor

Clinical evaluation and genetic analysis of patients affected by premature ovarian insufficiency: Identification and characterization of a new mutation of the BMP-15

2020 ◽  
Author(s):  
Francesca Orsolini ◽  
Eleonora Ferrarini ◽  
Patrizia Agretti ◽  
Marco Giuseppina De ◽  
Cosmo Caterina Di ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Clinical Evaluation ◽  
Premature Ovarian Insufficiency ◽  
Ovarian Insufficiency ◽  
New Mutation ◽  
Identification And Characterization

Identification and characterization of aldose reductase in cultured rat mesangial cells

Diabetes ◽  
1992 ◽  
Vol 41 (9) ◽  
pp. 1165-1171 ◽  
Author(s):  
R. Kikkawa ◽  
K. Umemura ◽  
M. Haneda ◽  
N. Kajiwara ◽  
S. Maeda ◽  
...  
Keyword(s):  
Aldose Reductase ◽  
Mesangial Cells ◽  
Identification And Characterization
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