Production and Transport of the Silencing Signal in Transgenic and Virus-Infected Plant Systems

Author(s):  
Thomas Hohn ◽  
Rashid Akbergenov ◽  
Mikhail M. Pooggin

Author(s):  
Russell L. Steere ◽  
Eric F. Erbe

It has been assumed by many involved in freeze-etch or freeze-fracture studies that it would be useless to etch specimens which were cryoprotected by more than 15% glycerol. We presumed that the amount of cryoprotective material exposed at the surface would serve as a contaminating layer and prevent the visualization of fine details. Recent unexpected freeze-etch results indicated that it would be useful to compare complementary replicas in which one-half of the frozen-fractured specimen would be shadowed and replicated immediately after fracturing whereas the complement would be etched at -98°C for 1 to 10 minutes before being shadowed and replicated.Standard complementary replica holders (Steere, 1973) with hinges removed were used for this study. Specimens consisting of unfixed virus-infected plant tissue infiltrated with 0.05 M phosphate buffer or distilled water were used without cryoprotectant. Some were permitted to settle through gradients to the desired concentrations of different cryoprotectants.



Virology ◽  
1965 ◽  
Vol 26 (2) ◽  
pp. 180-189 ◽  
Author(s):  
Samuel H Smith ◽  
David E Schlegel


1984 ◽  
Vol 3 (2) ◽  
pp. 309-314 ◽  
Author(s):  
M. Volovitch ◽  
N. Modjtahedi ◽  
P. Yot ◽  
G. Brun




1998 ◽  
Vol 90 (3) ◽  
pp. 280-280
Author(s):  
Odette Rohfritsch ◽  
Fabien Gaire ◽  
Corinne Schmitt ◽  
Lothaire Pinck






Author(s):  
Ryo Sugiura ◽  
Shogo Tsuda ◽  
Hiroyuki Tsuji ◽  
Noriyuki Murakami


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