Production and Transport of the Silencing Signal in Transgenic and Virus-Infected Plant Systems

2007 ◽  
pp. 127-157 ◽  
Author(s):  
Thomas Hohn ◽  
Rashid Akbergenov ◽  
Mikhail M. Pooggin
Keyword(s):  
Infected Plant ◽  
Virus Infected Plant

Freeze-fracture, freeze-etch complementary pairs of virus-infected cells reveal value of etching even when relatively high concentrations of cryoprotectants are used

1978 ◽  
Vol 36 (2) ◽  
pp. 136-137
Author(s):  
Russell L. Steere ◽  
Eric F. Erbe
Keyword(s):  
Plant Tissue ◽  
Phosphate Buffer ◽  
Infected Plant ◽  
Freeze Fracture ◽  
Distilled Water ◽  
Virus Infected Plant ◽  
Infected Cells ◽  
High Concentrations

It has been assumed by many involved in freeze-etch or freeze-fracture studies that it would be useless to etch specimens which were cryoprotected by more than 15% glycerol. We presumed that the amount of cryoprotective material exposed at the surface would serve as a contaminating layer and prevent the visualization of fine details. Recent unexpected freeze-etch results indicated that it would be useful to compare complementary replicas in which one-half of the frozen-fractured specimen would be shadowed and replicated immediately after fracturing whereas the complement would be etched at -98°C for 1 to 10 minutes before being shadowed and replicated.Standard complementary replica holders (Steere, 1973) with hinges removed were used for this study. Specimens consisting of unfixed virus-infected plant tissue infiltrated with 0.05 M phosphate buffer or distilled water were used without cryoprotectant. Some were permitted to settle through gradients to the desired concentrations of different cryoprotectants.

Ultrastucture of membranes induced in Grapevine Fanleaf virus-infected plant cells

1998 ◽  
Vol 90 (3) ◽  
pp. 280-280
Author(s):  
Odette Rohfritsch ◽  
Fabien Gaire ◽  
Corinne Schmitt ◽  
Lothaire Pinck
Keyword(s):  
Infected Plant ◽  
Plant Cells ◽  
Grapevine Fanleaf Virus ◽  
Virus Infected Plant

<i>Virus-Infected Plant Detection in Potato Seed Production Field by UAV Imagery</i>

2018 ◽  
Author(s):  
Ryo Sugiura ◽  
Shogo Tsuda ◽  
Hiroyuki Tsuji ◽  
Noriyuki Murakami
Keyword(s):  
Seed Production ◽  
Infected Plant ◽  
Potato Seed ◽  
Virus Infected Plant
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