Virus-Induced Gene Silencing for Functional Genomics in Withania somnifera, an Important Indian Medicinal Plant

2020 ◽  
pp. 139-154
Author(s):  
Dikki Pedenla Bomzan ◽  
H. B. Shilpashree ◽  
P. Anjali ◽  
Sarma Rajeev Kumar ◽  
Dinesh A. Nagegowda
Keyword(s):  
Medicinal Plant ◽  
Gene Silencing ◽  
Functional Genomics ◽  
Withania Somnifera ◽  
Virus Induced Gene Silencing ◽  
Indian Medicinal Plant

scholarly journals Agrobacterium-Mediated Gene Transient Overexpression and Tobacco Rattle Virus (TRV)-Based Gene Silencing in Cassava

2019 ◽  
Vol 20 (16) ◽  
pp. 3976 ◽  
Author(s):  
Hongqiu Zeng ◽  
Yanwei Xie ◽  
Guoyin Liu ◽  
Yunxie Wei ◽  
Wei Hu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Functional Genomics ◽  
Transient Expression ◽  
Fluorescent Protein ◽  
Tobacco Rattle Virus ◽  
Mosaic Disease ◽  
African Cassava Mosaic Virus ◽  
Cassava Mosaic Disease ◽  
Virus Induced Gene Silencing ◽  
Transient Expression Assay

Agrobacterium-mediated transient expression and virus-induced gene silencing (VIGS) are very useful in functional genomics in plants. However, whether these methods are effective in cassava (Manihot esculenta), one of the most important tropical crops, remains elusive. In this study, we used green fluorescent protein (GFP) and β-glucuronidase (GUS) as reporter genes in a transient expression assay. GFP or GUS could be detected in the infiltrated leaves at 2 days postinfiltration (dpi) and were evidenced by visual GFP and GUS assays, reverse-transcription PCR, and Western blot. In addition, phytoene desaturase (PDS) was used to show the silencing effect in a VIGS system. Both Agrobacterium GV3101 and AGL-1 with tobacco rattle virus (TRV)-MePDS-infiltrated distal leaves showed an albino phenotype at 20 dpi; in particular, the AGL-1-infiltrated plants showed an obvious albino area in the most distal leaves. Moreover, the silencing effect was validated by molecular identification. Notably, compared with the obvious cassava mosaic disease symptom infiltrated by African-cassava-mosaic-virus-based VIGS systems in previous studies, TRV-based VIGS-system-infiltrated cassava plants did not show obvious virus-induced disease symptoms, suggesting a significant advantage. Taken together, these methods could promote functional genomics in cassava.

scholarly journals An efficient virus-induced gene silencing (VIGS) system for functional genomics in Brassicas using a cabbage leaf curl virus (CaLCuV)-based vector

Planta ◽  
2020 ◽  
Vol 252 (3) ◽  
Author(s):  
Zhiliang Xiao ◽  
Miaomiao Xing ◽  
Xing Liu ◽  
Zhiyuan Fang ◽  
Limei Yang ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Functional Genomics ◽  
Virus Induced Gene Silencing ◽  
Cabbage Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

Virus-induced gene silencing using a modified betasatellite: a potential candidate for functional genomics of crops

2014 ◽  
Vol 159 (8) ◽  
pp. 2109-2113 ◽  
Author(s):  
Jitendra Kumar ◽  
Samatha Gunapati ◽  
Jitesh Kumar ◽  
Anita Kumari ◽  
Abhinav Kumar ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Functional Genomics ◽  
Potential Candidate ◽  
Virus Induced Gene Silencing

scholarly journals Virus-induced gene silencing for Asteraceae-a reverse genetics approach for functional genomics in Gerbera hybrida

2012 ◽  
Vol 10 (8) ◽  
pp. 970-978 ◽  
Author(s):  
Xianbao Deng ◽  
Paula Elomaa ◽  
Cuong X. Nguyen ◽  
Timo Hytönen ◽  
Jari P. T. Valkonen ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Functional Genomics ◽  
Reverse Genetics ◽  
Virus Induced Gene Silencing ◽  
Gerbera Hybrida

Graft-accelerated virus-induced gene silencing facilitates functional genomics in rose flowers

2017 ◽  
Vol 60 (1) ◽  
pp. 34-44 ◽  
Author(s):  
Huijun Yan ◽  
Shaochuan Shi ◽  
Nan Ma ◽  
Xiaoqian Cao ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Functional Genomics ◽  
Virus Induced Gene Silencing

Virus-induced gene silencing as a tool for functional genomics in a legume species

2004 ◽  
Vol 40 (4) ◽  
pp. 622-631 ◽  
Author(s):  
Gabriela D. Constantin ◽  
Britta N. Krath ◽  
Stuart A. MacFarlane ◽  
Mogens Nicolaisen ◽  
Ida Elisabeth Johansen ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Functional Genomics ◽  
Legume Species ◽  
Virus Induced Gene Silencing

scholarly journals An efficient virus-induced gene silencing vector for maize functional genomics research

2016 ◽  
Vol 86 (1) ◽  
pp. 102-115 ◽  
Author(s):  
Rong Wang ◽  
Xinxin Yang ◽  
Nian Wang ◽  
Xuedong Liu ◽  
Richard S. Nelson ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Functional Genomics ◽  
Virus Induced Gene Silencing ◽  
Genomics Research
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