Measurement of DNA Damage in Spermatozoa by TUNEL Assay

2011 ◽  
pp. 495-497
Author(s):  
Rakesh Sharma ◽  
Ashok Agarwal
Keyword(s):  
Dna Damage ◽  
Tunel Assay

TUNEL assay and SCSA determine different aspects of sperm DNA damage

Andrologia ◽  
2010 ◽  
Vol 42 (5) ◽  
pp. 305-313 ◽  
Author(s):  
R. Henkel ◽  
C. F. Hoogendijk ◽  
P. J. D. Bouic ◽  
T. F. Kruger
Keyword(s):  
Dna Damage ◽  
Tunel Assay ◽  
Sperm Dna Damage ◽  
Sperm Dna

Evaluation of Sperm DNA Fragmentation via Halosperm Technique and TUNEL Assay Before and After Cryopreservation

2019 ◽  
Vol 26 (12) ◽  
pp. 1575-1581 ◽  
Author(s):  
Senay Cankut ◽  
Turgay Dinc ◽  
Mehmet Cincik ◽  
Guler Ozturk ◽  
Belgin Selam
Keyword(s):  
Dna Damage ◽  
Dna Fragmentation ◽  
Tunel Assay ◽  
University Hospital ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Sperm Dna Fragmentation ◽  
Liquid Form ◽  
Sperm Dna Damage ◽  
Sperm Dna ◽  
Before And After

Aim: Human sperm DNA fragmentation is one of the factors suggested for male infertility. The ratio of sperm DNA damage in semen may adversely affect both the fertilization rate and the embryo development of in vitro fertilization/ intracytoplasmic sperm injection cycles. Sperm cryopreservation both increases the success rates in assisted reproductive techniques (ARTs) and contributes to the preservation of fertility before testis surgery, chemotherapy, and radiotherapy. The aim of the current study is to determine sperm DNA fragmentation, following cryopreservation. Methods: A cross-sectional, observational study was conducted at a university hospital infertility clinic. One hundred (n = 100) volunteer fertile men (ages between 21 and 39 years) with normozoospermic sperm parameters were involved in the current study. Sperm DNA damage was evaluated with the Halosperm technique and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Fresh samples were studied in liquid form. The remaining samples were kept frozen and then thawed after 1 month and reevaluated with the Halosperm technique and TUNEL assay. Results were then compared between the fresh and frozen samples. Results: Sperm DNA fragmentation results with the Halosperm technique both before and after cryopreservation were 25% (5%-65%) and 40% (6%-89%), respectively, with a statistically significant increase (15%; P < .001). Sperm DNA fragmentation results by TUNEL assay before and after cryopreservation were 17% (3%-43%) and 36% (7%-94%), respectively, with a statistically significant increase (19%; P <.001). Conclusion: The current data demonstrate increased sperm DNA damage after cryopreservation. Further studies may contribute to development of less harmful techniques and cryoprotectants in order to improve the results of ART.

scholarly journals An investigation of the potential effect of vacuoles in human sperm on DNA damage using a chromosome assay and the TUNEL assay

2011 ◽  
Vol 26 (5) ◽  
pp. 978-986 ◽  
Author(s):  
S. Watanabe ◽  
A. Tanaka ◽  
S. Fujii ◽  
H. Mizunuma ◽  
A. Fukui ◽  
...  
Keyword(s):  
Dna Damage ◽  
Human Sperm ◽  
Potential Effect ◽  
Tunel Assay

The TUNEL assay underestimates the incidence of DNA damage in pig sperm due to chromatin condensation

2021 ◽  
Author(s):  
Jordi Ribas-Maynou ◽  
Estela Garcia-Bonavila ◽  
Sergi Bonet ◽  
Jaime Catalán ◽  
Albert Salas-Huetos ◽  
...  
Keyword(s):  
Dna Damage ◽  
Chromatin Condensation ◽  
Tunel Assay

Testing sperm DNA damage by tunel assay in specific cases of male infertility

2010 ◽  
Vol 94 (4) ◽  
pp. S146 ◽  
Author(s):  
R.K. Sharma ◽  
S. Gupta ◽  
A. Thiyagarajan ◽  
E. Sabanegh ◽  
A. Agarwal
Keyword(s):  
Dna Damage ◽  
Male Infertility ◽  
Tunel Assay ◽  
Sperm Dna Damage ◽  
Sperm Dna

Assessment of DNA damage in goat preantral follicles after vitrification of the ovarian cortex

2015 ◽  
Vol 27 (3) ◽  
pp. 440 ◽  
Author(s):  
Luciana R. Faustino ◽  
Adeline A. Carvalho ◽  
Cleidson M. G. Silva ◽  
Rafael Rossetto ◽  
Cláudio A. P. Lopes ◽  
...  
Keyword(s):  
Dna Damage ◽  
Ovarian Tissue ◽  
Tunel Assay ◽  
Lower Percentage ◽  
Dna Integrity ◽  
Preantral Follicles ◽  
Histone H2ax ◽  
Γh2ax Foci ◽  
Control Samples

Effective methods for gamete preservation should have low impact on DNA integrity. The present study investigated the effects of vitrification of goat ovarian tissues on the occurrence of DNA fragmentation and DNA double-stand breaks using the terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling (TUNEL) assay and detection of phosphorylated histone H2AX (γH2AX), respectively. Goat ovaries were collected at a local abattoir and 12 tissue fragments were prepared from each ovarian pair. Tissue fragments were used as fresh control samples or were cultured in vitro, vitrified or vitrified and cultured. Vitrification was performed using the Ovarian Tissue Cryosystem. Fragments from all groups (control and treatments) were processed for histology, transmission electron microscopy, TUNEL assay and immunofluorescence. Compared with fresh control samples, a lower percentage of morphologically normal follicles was detected in the vitrification followed by culture treatment group (P < 0.05). Normal follicular ultrastructure was observed in all groups. Immunofluorescence revealed the presence of γH2AX foci in few oocytes and ovarian stromal cells. TUNEL-positive follicles were found in samples without significant differences among groups (P > 0.05). In conclusion, the vitrification protocol used in the present study did not increase DNA damage in preantral follicles enclosed in goat ovarian tissues.

scholarly journals Evaluation of sperm DNA damage in bulls by TUNEL assay as a parameter of semen quality

2015 ◽  
Vol 61 (3) ◽  
pp. 185-190 ◽  
Author(s):  
Kumiko TAKEDA ◽  
Kyoko UCHIYAMA ◽  
Masashi KINUKAWA ◽  
Takahiro TAGAMI ◽  
Masahiro KANEDA ◽  
...  
Keyword(s):  
Dna Damage ◽  
Semen Quality ◽  
Tunel Assay ◽  
Sperm Dna Damage ◽  
Sperm Dna

Measurement of DNA Damage in Spermatozoa by TUNEL Assay

2013 ◽  
pp. 429-432
Author(s):  
Rakesh Sharma ◽  
Ashok Agarwal
Keyword(s):  
Dna Damage ◽  
Tunel Assay
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