Isolation and Characterization of Full Length cDNA Coding for the H23 Breast Tumor Associated Antigen

1989 ◽  
pp. 41-59 ◽  
Author(s):  
D. H. Wreschner ◽  
I. Tsarfaty ◽  
M. Hareuveni ◽  
J. Zaretsky ◽  
N. Smorodinsky ◽  
...  
Keyword(s):  
Breast Tumor ◽  
Full Length ◽  
Full Length Cdna ◽  
Isolation And Characterization ◽  
Tumor Associated Antigen

A Chitinase Encoding Gene ( chit1 Gene) from the Entomopathogen Metarhizium anisopliae : Isolation and Characterization of Genomic and Full-Length cDNA

1998 ◽  
Vol 37 (4) ◽  
pp. 221-225 ◽  
Author(s):  
Maurício Reis Bogo ◽  
Cláudia Augustin Rota ◽  
Hermides Pinto Jr. ◽  
Maristela Ocampos ◽  
Cláudia Tatiana Correa ◽  
...  
Keyword(s):  
Metarhizium Anisopliae ◽  
Full Length ◽  
Full Length Cdna ◽  
Isolation And Characterization ◽  
Encoding Gene

scholarly journals Isolation and characterization of a full-length cDNA encoding the 55-kDa rabbit zona pellucida protein.

1991 ◽  
Vol 266 (11) ◽  
pp. 7214-7219
Author(s):  
E Schwoebel ◽  
S Prasad ◽  
T M Timmons ◽  
R Cook ◽  
H Kimura ◽  
...  
Keyword(s):  
Zona Pellucida ◽  
Full Length ◽  
Full Length Cdna ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of a Rice Full-Length cDNA Clone Encoding a Polyubiquitin

1994 ◽  
Vol 106 (2) ◽  
pp. 791-792 ◽  
Author(s):  
Y. M. Kim ◽  
J. K. Kim ◽  
Y. S. Hwang
Keyword(s):  
Cdna Clone ◽  
Full Length ◽  
Full Length Cdna ◽  
Isolation And Characterization

Cloning and Characterization of a Flavanone 3-Hydroxylase Gene from Ginkgo biloba

2006 ◽  
Vol 26 (1) ◽  
pp. 19-29 ◽  
Author(s):  
Guoan Shen ◽  
Yongzhen Pang ◽  
Weisheng Wu ◽  
Zhongxiang Deng ◽  
Lingxia Zhao ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Genomic Dna ◽  
Dna Analysis ◽  
Full Length ◽  
Dimensional Structure ◽  
Typical Structure ◽  
Transcription Analysis ◽  
Full Length Cdna ◽  
Isolation And Characterization

Flavanone 3-hydroxylase (F3H) activity is necessary for the biosynthesis of flavonoids, the main ingredients of Gingko biloba extract. The full-length cDNA and genomic DNA sequences of F3H gene were isolated from G. biloba for the first time. The full-length cDNA of G. biloba F3H gene (designated as GbF3H) contained a 1071 bp open reading frame (ORF) encoding a 357-amino-acid protein with a calculated molecular weight of about 40 kDa and isoelectric point (pI) of 5.57. The genomic DNA analysis showed that GbF3H gene had three exons and two introns. The deduced GbF3H protein showed high identities to other plant F3Hs. The conserved amino acids ligating ferrous iron and residues participating in 2-oxoglutarate binding (R-X-S) were found in GbF3H at the similar positions like other F3Hs. Three-dimensional structure modeling showed that GbF3H had a jerry roll in the enzyme core consisted of β-sheet, a typical structure shared by all 2-oxoglutarate-dependent dioxygenases including F3Hs. Phylogenetic tree analysis revealed that GbF3H shared the same ancestor in evolution with other F3Hs and had a further relationship with other angiosperms species. Southern blot analysis indicated that GbF3H belonged to a multi-gene family. Transcription analysis revealed that GbF3H expressed in stem and leaf with the highest transcription level in leaf. The isolation and characterization of GbF3H gene will be helpful to further study the role of GbF3H gene in the biosynthesis of flavonoids in G. biloba.

scholarly journals Isolation and characterization of full-length cDNA clones coding for cholinesterase from fetal human tissues.

1987 ◽  
Vol 84 (11) ◽  
pp. 3555-3559 ◽  
Author(s):  
C. A. Prody ◽  
D. Zevin-Sonkin ◽  
A. Gnatt ◽  
O. Goldberg ◽  
H. Soreq
Keyword(s):  
Full Length ◽  
Cdna Clones ◽  
Human Tissues ◽  
Full Length Cdna ◽  
Isolation And Characterization

Krabbe Disease: Isolation and Characterization of a Full-Length cDNA for Human Galactocerebrosidase

1994 ◽  
Vol 198 (2) ◽  
pp. 485-491 ◽  
Author(s):  
N. Sakai ◽  
K. Inui ◽  
N. Fujii ◽  
H. Fukushima ◽  
J. Nishimoto ◽  
...  
Keyword(s):  
Full Length ◽  
Krabbe Disease ◽  
Full Length Cdna ◽  
Isolation And Characterization

Cloning and characterization of full-length cDNA clones encoding chalcone synthase from the orchid Bromheadia finlaysoniana

1998 ◽  
Vol 36 (9) ◽  
pp. 647-656 ◽  
Author(s):  
Chye-Fong Liew ◽  
Chong-Jin Goh ◽  
Chiang-Shiong Loh ◽  
Saw-Hoon Lim
Keyword(s):  
Chalcone Synthase ◽  
Full Length ◽  
Cdna Clones ◽  
Full Length Cdna
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