Nodule Culture: A Developmental Pathway with High Potential for Regeneration, Automated Micropropagation, and Plant Metabolite Production from Woody Plants

Author(s):  
Brent H. McCown ◽  
Eric L. Zeldin ◽  
Hamilton A. Pinkalla ◽  
Richard R. Dedolph
Author(s):  
Kadriye Özcan

In this study, active secondary metabolite production capacity of actinomycete isolates obtained from Trabzon (Black Sea) sea sediments was investigated by molecular techniques. Totaly 24 actinomycetes were investigated by PCR based on the presence of secondary metabolite biosynthesis genes PKS / NRPS. According to the PCR results, 25 and 58% of actinomycetes obtained from Trabzon sea sediments were found to contain PKS-NRPS and only NRPS gene regions, respectively. When PCR data were evaluated, it was found that the production of the peptide form active secondary metabolite of the isolates by non-ribosomal way was higher than that of the secondary metabolite production by the PKS pathway. In addition, it has been determined that Black Sea marine sediments have high potential for active secondary metabolite production.


2021 ◽  
Vol 875 (1) ◽  
pp. 012053
Author(s):  
I Vasyukova ◽  
A Gusev ◽  
O Zakharova ◽  
P Baranchikov ◽  
N Yevtushenko

Abstract The main purpose of the work was to determine the prospects for the use of silver nanoparticles in woody plants tissue culture and to identify possible limitations. The paper presents the results of experiments on the use of silver nanoparticles (AgNPs) to increase the efficiency of in vitro tissue culture of woody plant cultures using the example of gray poplar (Populus × canescens Aiton. Sm.) at various stages of microclonal reproduction. It has been shown that sequential treatment with 7.5 g/L NaOCl for 30 min and 0.3 g/L AgNPs for 5 min allows obtaining 100% phytopathogen-free explants, increasing their viability. Modification of the nutrient medium with AgNPs at concentrations of 1.5…3 μg/L during multiplication and rooting reduces the phytopathogen infestation, and also stimulates the formation of the root system and accelerates the growth of the vegetative part of the shoots. In addition, the combination of photosynthetic and enzymatic activity suggests a rather high potential for stress resistance of experimental microclones in comparison with control ones.


1993 ◽  
Vol 89 (1) ◽  
pp. 165-171 ◽  
Author(s):  
Douglas G. Muench ◽  
O. William Archibold ◽  
Allen G. Good

2009 ◽  
Author(s):  
Steve Krupp ◽  
Orla M. NicDomhnaill ◽  
Allan H. Church ◽  
Steven J. Robison ◽  
Michael N. Bazigos

2009 ◽  
Author(s):  
Michel A. Buffet ◽  
Robin R. Cohen ◽  
Michael Crespo ◽  
Stephen A. Dwight ◽  
Lorry A. Olson
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