Comparison of Quantitative and Immunohistochemistry for Choline Acetyltransferase in the Rat Cochlear Nucleus

1993 ◽  
pp. 267-278 ◽  
Author(s):  
Donald A. Godfrey
Keyword(s):  
Choline Acetyltransferase ◽  
Cochlear Nucleus

scholarly journals Quantitative distribution of choline acetyltransferase and acetylcholinesterase activities in the rat cochlear nucleus.

1981 ◽  
Vol 29 (6) ◽  
pp. 720-730 ◽  
Author(s):  
D A Godfrey ◽  
F M Matschinsky
Keyword(s):  
Choline Acetyltransferase ◽  
Cochlear Nucleus ◽  
Nerve Root ◽  
Cholinergic System ◽  
Cholinergic Neurons ◽  
Cholinergic Interneurons ◽  
Uniform Distributions ◽  
Construct Maps ◽  
Nerve System ◽  
Very High

Within the cochlear nucleus of the rat, as well as some nearby regions, quantitative histochemical mapping procedures were used to construct maps of the distributions of choline acetyltransferase and acetylcholinesterase activities. The results were in some ways consistent with results previously reported for cat, e.g., very low activities of both enzymes were found in the auditory nerve root, and also in the vestibular nerve root, except where acetylcholinesterase-positive centrifugal fibers are located, very high activities were found in the facial nerve system. In many ways, however, the results for the rat cochlear nucleus contrasted with those for the cat. Notably, choline acetyltransferase activities in some regions of the rat cochlear nucleus were as much as 30- to 60-fold higher than for the comparable regions in the cat, and both enzymes had much more uniform distributions in the rat cochlear nucleus than in the cat. The more prominent cholinergic system in the rat cochlear nucleus might relate to a proportionately larger population of cholinergic interneurons, or, more probably, a more significant innervation by cholinergic, centrifugal pathways, or both, as well as, perhaps, generally higher choline acetyltransferase activities in cholinergic neurons of rat.

Effects of intense tone exposure on choline acetyltransferase activity in the hamster cochlear nucleus

2006 ◽  
Vol 216-217 ◽  
pp. 168-175 ◽  
Author(s):  
Yong-Ming Jin ◽  
Donald A. Godfrey ◽  
Jie Wang ◽  
James A. Kaltenbach
Keyword(s):  
Choline Acetyltransferase ◽  
Cochlear Nucleus ◽  
Acetyltransferase Activity ◽  
Choline Acetyltransferase Activity

Contribution of centrifugal innervation to choline acetyltransferase activity in the cat cochlear nucleus

1990 ◽  
Vol 49 (1-3) ◽  
pp. 259-279 ◽  
Author(s):  
Donald A Godfrey ◽  
Katrina L Beranek ◽  
Lissette Carlson ◽  
Judy A Parli ◽  
Jon D Dunn ◽  
...  
Keyword(s):  
Choline Acetyltransferase ◽  
Cochlear Nucleus ◽  
Acetyltransferase Activity ◽  
Choline Acetyltransferase Activity

Effect of olivocochlear bundle transection on choline acetyltransferase activity in the rat cochlear nucleus

1987 ◽  
Vol 28 (2-3) ◽  
pp. 237-251 ◽  
Author(s):  
Donald A. Godfrey ◽  
Jami L. Park-Hellendall ◽  
Jon D. Dunn ◽  
C.David Ross
Keyword(s):  
Choline Acetyltransferase ◽  
Cochlear Nucleus ◽  
Acetyltransferase Activity ◽  
Olivocochlear Bundle ◽  
Choline Acetyltransferase Activity

Ultrastructural Localization of Acetylcholinesterase in the Arcuate Nucleus and Median Eminence of the Rat

1977 ◽  
Vol 35 ◽  
pp. 440-441
Author(s):  
K.A. Carson ◽  
C.B. Nemeroff ◽  
M.S. Rone ◽  
J.S. Kizer ◽  
J.S. Hanker
Keyword(s):  
Choline Acetyltransferase ◽  
Median Eminence ◽  
Arcuate Nucleus ◽  
Cholinergic Neurons ◽  
Ultrastructural Localization ◽  
Male Rats ◽  
Neonatal Rats ◽  
Good Marker ◽  
Chemical Studies ◽  
High Doses

Biochemical, physiological, pharmacological, and more recently enzyme histo- chemical data have indicated that cholinergic circuits exist in the hypothalamus. Ultrastructural correlates of these pathways such as acetylcholinesterase (AchE) positive neurons in the arcuate nucleus (ARC) and stained terminals in the median eminence (ME) have yet to be described. Initial studies in our laboratories utilizing chemical lesioning and microdissection techniques coupled with microchemical and light microscopic enzyme histo- chemical studies suggested the existence of cholinergic neurons in the ARC which project to the ME (1). Furthermore, in adult male rats with Halasz deafferentations (hypothalamic islands composed primarily of the isolated ARC and the ME) choline acetyltransferase (ChAc) activity, a good marker for cholinergic neurons, was not significantly reduced in the ME and was only somewhat reduced in the ARC (2). Treatment of neonatal rats with high doses of monosodium 1-glutamate (MSG) results in a lesion largely restricted to the neurons of the ARC.

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