New mechanisms of gene amplification in drug resistance (the episome model)

1991 ◽  
pp. 1-11 ◽  
Author(s):  
Daniel D. Von Hoff
Keyword(s):  
Drug Resistance ◽  
Gene Amplification

Electrophoretic analysis of 248 clinical breast cancer specimens for P-glycoprotein overexpression or gene amplification.

1989 ◽  
Vol 7 (8) ◽  
pp. 1129-1136 ◽  
Author(s):  
D E Merkel ◽  
S A Fuqua ◽  
A K Tandon ◽  
S M Hill ◽  
A U Buzdar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Resistance ◽  
Gene Amplification ◽  
Multiple Drug Resistance ◽  
Electrophoretic Analysis ◽  
Gene Copy ◽  
Cross Resistance ◽  
Glycoprotein Gene ◽  
P Glycoprotein ◽  
Clinical Breast

Multiple drug resistance (MDR), consisting of acquired cross resistance to anthracyclines, vinca alkyloids, and other antineoplastic antibiotics, has been described in a variety of cell lines. This MDR phenotype is associated with overexpression and sometimes amplification of a gene coding for a 170 kDa glycoprotein, termed P-glycoprotein. To understand the role of this mechanism in clinical breast cancer, 248 breast cancer specimens representing both untreated primary and refractory relapsing disease were probed for evidence of P-glycoprotein gene amplification or overexpression using Southern, Northern, or Western blot techniques. In no case was an increase in P-glycoprotein gene copy number or expression detected. Though these findings do not necessarily rule out a role for P-glycoprotein in mediating drug resistance in breast cancer, electrophoretic analysis of clinical specimens is unlikely to provide useful predictive information. More sensitive assays must be developed to overcome the difficulties inherent in analyzing heterogenous tissue samples.

Gene amplification and drug resistance

1991 ◽  
Vol 163 (4) ◽  
pp. 287-292 ◽  
Author(s):  
R. Brown
Keyword(s):  
Drug Resistance ◽  
Gene Amplification

Gene amplification and drug resistance in cultured murine cells

Science ◽  
1978 ◽  
Vol 202 (4372) ◽  
pp. 1051-1055 ◽  
Author(s):  
R. Schimke ◽  
R. Kaufman ◽  
F. Alt ◽  
R. Kellems
Keyword(s):  
Drug Resistance ◽  
Gene Amplification

Gene amplification and drug resistance in cultured animal cells

1984 ◽  
Vol 11 ◽  
pp. 9-17 ◽  
Author(s):  
R.T. Schimke ◽  
S. Beverley ◽  
P. Brown ◽  
R. Cassin ◽  
N. Federspiel ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Gene Amplification ◽  
Animal Cells
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