On the Mode of Action of the Delta-Endotoxin of Bacillus Thuringiensis

1985 ◽  
pp. 131-147
Author(s):  
Maja Huber-Lukač
Keyword(s):  
Bacillus Thuringiensis ◽  
Mode Of Action ◽  
Delta Endotoxin

Mode of action of CryIIA: a Bacillus thuringiensis delta-endotoxin

1994 ◽  
Vol 24 (10) ◽  
pp. 1025-1035 ◽  
Author(s):  
L ENGLISH ◽  
H ROBBINS ◽  
M VONTERSCH ◽  
C KULESZA ◽  
D AVE ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Mode Of Action ◽  
Delta Endotoxin

scholarly journals The Bacillus thuringiensis delta-endotoxin. Evidence for a two domain structure of the minimal toxic fragment.

1990 ◽  
Vol 265 (3) ◽  
pp. 1369-1375
Author(s):  
D Convents ◽  
C Houssier ◽  
I Lasters ◽  
M Lauwereys
Keyword(s):  
Bacillus Thuringiensis ◽  
Domain Structure ◽  
Delta Endotoxin

scholarly journals Analysis of the molecular basis of insecticidal specificity of Bacillus thuringiensis crystal δ-endotoxin

1987 ◽  
Vol 248 (1) ◽  
pp. 197-201 ◽  
Author(s):  
M Z Haider ◽  
D J Ellar
Keyword(s):  
Bacillus Thuringiensis ◽  
Membrane Proteins ◽  
Insect Cell ◽  
Receptor Interaction ◽  
Dual Specificity ◽  
Delta Endotoxin ◽  
Cell Membrane Proteins ◽  
Toxin Receptor ◽  
Osmotic Lysis ◽  
Initial Interaction

The mechanism of action and receptor binding of a dual-specificity Bacillus thuringiensis var. aizawai ICl delta-endotoxin was studied using insect cell culture. The native protoxin was labelled with 125I, proteolytically activated and the affinity of the resulting preparations for insect cell-membrane proteins was studied by blotting. The active preparations obtained by various treatments had characteristic specificity associated with unique polypeptides, and showed affinity for different membrane proteins. The lepidopteran-specific preparation (trypsin-treated protoxin containing 58 and 55 kDa polypeptides) bound to two membrane proteins in the lepidopteran cells but none in the dipteran cells. The dipteran-specific preparation (protoxin treated sequentially with trypsin and Aedes aegypti gut proteases, containing a 53 kDa polypeptide) bound to a 90 kDa membrane protein in the dipteran (A. aegypti) cells but bound to none in the lepidopteran cells or Drosophila melanogaster cells. The toxicity of trypsin-activated delta-endotoxin was completely inhibited by preincubation with D-glucose, suggesting a role for this carbohydrate in toxin-receptor interaction. The toxicity was also decreased by osmotic protectants to an extent proportional to their viscometric radius. These results support a proposal that initial interaction of toxin with a unique receptor determines the specificity of the toxin, following which cell death occurs by a mechanism of colloid osmotic lysis.

scholarly journals Effects of Bacillus thuringiensis δ-Endotoxin on Insect and Mammalian Cells In Vitro

1980 ◽  
Vol 15 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Junko NISHITSUTSUJI-UWO ◽  
Yasuhisa ENDO ◽  
Michio HIMENO
Keyword(s):  
Bacillus Thuringiensis ◽  
Mammalian Cells ◽  
Delta Endotoxin

scholarly journals Plasmids and insecticidal activity of delta-endotoxin crystals from Bacillus thuringiensis var. israelensis.

1985 ◽  
Vol 49 (3) ◽  
pp. 573-580 ◽  
Author(s):  
Michio HIMENO ◽  
Masao IKEDA ◽  
Kikuo SEN ◽  
Naoto KOYAMA ◽  
Tohru KOMANO ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insecticidal Activity ◽  
Delta Endotoxin
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