Pronuclear Microinjection of One-Cell Embryos

2019 ◽  
pp. 27-33
Author(s):  
Melissa A. Larson
Keyword(s):  
Pronuclear Microinjection

Transgenic mouse production: pipette cleaning for pronuclear microinjection

1988 ◽  
Vol 4 (6) ◽  
pp. 152-152
Author(s):  
J CARNWATH ◽  
J OSULLIVAN ◽  
G SCHMIDT
Keyword(s):  
Transgenic Mouse ◽  
Pronuclear Microinjection

State of the art in the production of transgenic goats

2004 ◽  
Vol 16 (4) ◽  
pp. 465 ◽  
Author(s):  
H. Baldassarre ◽  
B. Wang ◽  
C. L. Keefer ◽  
A. Lazaris ◽  
C. N. Karatzas
Keyword(s):  
Recombinant Proteins ◽  
Traditional Method ◽  
Nuclear Transfer ◽  
State Of The Art ◽  
Expression Vectors ◽  
Marketing Approval ◽  
Transfected Cells ◽  
Pronuclear Microinjection ◽  
Transgenic Goats

This review summarises recent advances in the field of transgenic goats for the purpose of producing recombinant proteins in their milk. Production of transgenic goats via pronuclear microinjection of DNA expression vectors has been the traditional method, but this results in low efficiencies. Somatic cell nuclear transfer has dramatically improved efficiencies in rates of transgenesis. Characterisation of transfected cells in vitro before use in nuclear transfer guarantees that kids born are transgenic and of predetermined gender. Using these platform technologies, several recombinant proteins of commercial interest have been produced, although none of them has yet gained marketing approval. Before these technologies are implemented in goat improvement programmes, efficiencies must be improved, costs reduced, and regulatory approval obtained for the marketing of food products derived from such animals.

scholarly journals Generation of Mutant Pigs by Direct Pronuclear Microinjection of CRISPR/Cas9 Plasmid Vectors

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (11) ◽  
Author(s):  
Chin-kai Chuang ◽  
Ching-Fu Tu ◽  
Chien-Hong Chen
Keyword(s):  
Plasmid Vectors ◽  
Pronuclear Microinjection

culture of sheep zygotes following pronuclear microinjection

1990 ◽  
Vol 33 (1) ◽  
pp. 342
Author(s):  
S.K. Walker ◽  
T.M. Heard ◽  
P.J. Verma ◽  
C.S. Bawden ◽  
A.V. Sivaprasad ◽  
...  
Keyword(s):  
Pronuclear Microinjection

scholarly journals Activation of Mouse Metallothionein-I Promoter in Mouse Preimplantation Embryos After Pronuclear Microinjection.

1997 ◽  
Vol 43 (4) ◽  
pp. 321-328 ◽  
Author(s):  
Satoshi TAKEDA ◽  
Sadahiro AZUMA ◽  
Tohru TSUKUI ◽  
Yutaka TOYODA
Keyword(s):  
Preimplantation Embryos ◽  
Pronuclear Microinjection

Production of transgenic goats by pronuclear microinjection of in vitro produced zygotes derived from oocytes recovered by laparoscopy

2003 ◽  
Vol 59 (3-4) ◽  
pp. 831-839 ◽  
Author(s):  
Hernan Baldassarre ◽  
Bin Wang ◽  
Nacereddine Kafidi ◽  
Melanie Gauthier ◽  
Nathalie Neveu ◽  
...  
Keyword(s):  
Pronuclear Microinjection ◽  
Transgenic Goats

Pronuclear Microinjection

2001 ◽  
Vol 3 (4) ◽  
pp. 209-220 ◽  
Author(s):  
Robert J. Wall
Keyword(s):  
Pronuclear Microinjection

Production of Transgenic Mice by Pronuclear Microinjection

2018 ◽  
pp. 17-41 ◽  
Author(s):  
Xin-an Pu ◽  
Anthony P. Young ◽  
H. Michael Kubisch
Keyword(s):  
Transgenic Mice ◽  
Pronuclear Microinjection
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