Synthetic Antibody Libraries

2012 ◽  
pp. 27-41 ◽  
Author(s):  
Bryce Nelson ◽  
Sachdev S. Sidhu
Keyword(s):  
Synthetic Antibody ◽  
Antibody Libraries

scholarly journals Reduction of Nonspecificity Motifs in Synthetic Antibody Libraries

2018 ◽  
Vol 430 (1) ◽  
pp. 119-130 ◽  
Author(s):  
Ryan L. Kelly ◽  
Doris Le ◽  
Jessie Zhao ◽  
K. Dane Wittrup
Keyword(s):  
Synthetic Antibody ◽  
Antibody Libraries

scholarly journals Synthetic Antibody Libraries Focused Towards Peptide Ligands

2008 ◽  
Vol 378 (3) ◽  
pp. 622-633 ◽  
Author(s):  
Christian W. Cobaugh ◽  
Juan C. Almagro ◽  
Mark Pogson ◽  
Brent Iverson ◽  
George Georgiou
Keyword(s):  
Peptide Ligands ◽  
Synthetic Antibody ◽  
Antibody Libraries

scholarly journals Phage Display Selection of an Anti-Idiotype-Antibody with Broad-Specificity to Deoxynivalenol Mycotoxins

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 18
Author(s):  
Janne Leivo ◽  
Markus Vehniäinen ◽  
Urpo Lamminmäki
Keyword(s):  
In Vitro Selection ◽  
Specific Binding ◽  
Binding Properties ◽  
Phage Displays ◽  
Synthetic Antibody ◽  
Wide Range ◽  
Antibody Libraries ◽  
Rapid Generation ◽  
Selection Of

The use of synthetic antibody libraries and phage displays provides an efficient and robust method for the generation of antibodies against a wide range of targets with highly specific binding properties. As the in vitro selection conditions can be easily controlled, these methods enable the rapid generation of binders against difficult targets such as toxins and haptens. In this study, we used deoxynivalenol mycotoxin as a target to generate anti-idiotype-antibodies with unique binding properties from synthetic antibody libraries. The binding of the selected anti-idiotype antibodies can be efficiently inhibited with the addition of free isoforms of deoxynivalenol. The antibody was consecutively used to develop deoxynivalenol-specific ELISA and TRF-immunoassays, which can detect deoxynivalenol and two of the most common metabolic isoforms in the range of 78–115 ng/mL.

Selection of antibodies from synthetic antibody libraries

2012 ◽  
Vol 526 (2) ◽  
pp. 87-98 ◽  
Author(s):  
Noa Harel Inbar ◽  
Itai Benhar
Keyword(s):  
Synthetic Antibody ◽  
Antibody Libraries ◽  
Selection Of
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