Design and Generation of Synthetic Antibody Libraries for Phage Display

2014 ◽  
pp. 113-131 ◽  
Author(s):  
Gang Chen ◽  
Sachdev S. Sidhu
Keyword(s):  
Phage Display ◽  
Synthetic Antibody ◽  
Antibody Libraries

Phage display antibody libraries: A robust approach for generation of recombinant human monoclonal antibodies

2019 ◽  
Vol 135 ◽  
pp. 907-918 ◽  
Author(s):  
Rajesh Kumar ◽  
Hilal Ahmed Parray ◽  
Tripti Shrivastava ◽  
Subrata Sinha ◽  
Kalpana Luthra
Keyword(s):  
Monoclonal Antibodies ◽  
Phage Display ◽  
Human Monoclonal Antibodies ◽  
Robust Approach ◽  
Antibody Libraries ◽  
Phage Display Antibody

scholarly journals Abrogation of Viral Interleukin-6 (vIL-6)-Induced Signaling by Intracellular Retention and Neutralization of vIL-6 with an Anti-vIL-6 Single-Chain Antibody Selected by Phage Display

2006 ◽  
Vol 80 (17) ◽  
pp. 8510-8520 ◽  
Author(s):  
Marina Kovaleva ◽  
Ingo Bussmeyer ◽  
Björn Rabe ◽  
Joachim Grötzinger ◽  
Enge Sudarman ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Phage Display ◽  
Cell Line ◽  
Primary Effusion Lymphoma ◽  
Recombinant Antibody ◽  
Cytokine Signaling ◽  
Hepatoma Cell Line ◽  
Single Chain ◽  
Synthetic Antibody ◽  
Stat3 Phosphorylation

ABSTRACT Human herpesvirus 8 (HHV-8) encodes several putative oncogenes, which are homologues to cellular host genes known to function in cell cycle regulation, control of apoptosis, and cytokine signaling. Viral interleukin (vIL-6) is believed to play an important role in the pathogenesis of Kaposi's sarcoma as well as primary effusion lymphoma and multicentric Castleman's disease. Therefore, vIL-6 is a promising target for novel therapies directed against HHV-8-associated diseases. By phage display screening of human synthetic antibody libraries, we have selected a specific recombinant antibody, called monoclonal anti-vIL-6 (MAV), binding to vIL-6. The epitope recognized by MAV was localized on the top of the D helix of the vIL-6 protein, which is a part of receptor binding site III. Consequently, MAV specifically inhibits vIL-6-mediated growth of the primary effusion lymphoma-derived cell line BCBL-1 and blocks STAT3 phosphorylation in the human hepatoma cell line HepG2. Since it was previously found that vIL-6 can also induce signals from within the cell, presumably within the endoplasmic reticulum, we fused the recombinant antibody MAV with the endoplasmic retention sequence KDEL (MAV-KDEL). As a result, COS-7 cells expressing MAV-KDEL and synthesizing vIL-6 ceased to secrete the cytokine. Moreover, we observed that vIL-6 that was bound to MAV-KDEL and retained in the endoplasmic reticulum did not induce STAT3 phosphorylation in HepG2 cells. We conclude that the activity of the intracellularly retained vIL-6 protein is neutralized by MAV-KDEL. Our results might represent a novel therapeutic strategy to neutralize virally encoded growth factors or oncogenes.

Synthetic Antibody Libraries

2012 ◽  
pp. 27-41 ◽  
Author(s):  
Bryce Nelson ◽  
Sachdev S. Sidhu
Keyword(s):  
Synthetic Antibody ◽  
Antibody Libraries

scholarly journals Reduction of Nonspecificity Motifs in Synthetic Antibody Libraries

2018 ◽  
Vol 430 (1) ◽  
pp. 119-130 ◽  
Author(s):  
Ryan L. Kelly ◽  
Doris Le ◽  
Jessie Zhao ◽  
K. Dane Wittrup
Keyword(s):  
Synthetic Antibody ◽  
Antibody Libraries

scholarly journals Synthetic Antibody Libraries Focused Towards Peptide Ligands

2008 ◽  
Vol 378 (3) ◽  
pp. 622-633 ◽  
Author(s):  
Christian W. Cobaugh ◽  
Juan C. Almagro ◽  
Mark Pogson ◽  
Brent Iverson ◽  
George Georgiou
Keyword(s):  
Peptide Ligands ◽  
Synthetic Antibody ◽  
Antibody Libraries

Phage display of combinatorial antibody libraries

1997 ◽  
Vol 8 (4) ◽  
pp. 503-508 ◽  
Author(s):  
Christoph Rader ◽  
Carlos F Barbas
Keyword(s):  
Phage Display ◽  
Antibody Libraries
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