A Model of Electrokinetic Platform for Separation of Different Sizes of Biological Particles

2017 ◽  
pp. 118-128
Author(s):  
Reda Abdelbaset ◽  
Yehya H. Ghallab ◽  
Hamdy Abdelhamid ◽  
Yehea Ismail
Keyword(s):  
Biological Particles

scholarly journals OptiPrep™ Density Gradient Solutions for Mammalian Organelles

2002 ◽  
Vol 2 ◽  
pp. 1440-1443 ◽  
Author(s):  
John Graham
Keyword(s):  
Ionic Strength ◽  
Density Gradient ◽  
Biological Particles

Any density gradient for the isolation of mammalian organelles should ideally only expose the sedimenting biological particles to an increasing concentration of the gradient solute. Thus they will experience only an increasing density and viscosity, other parameters such as osmolality, pH, ionic strength and the concentration of important additives (such as EDTA and DTT) should remain as close to constant as possible. This Protocol Article describes the strategies for the dilution of OptiPrep™ in order to prepare such solutions for mammalian organelles and membranes.

Rapid identification of biological particles using on-the-fly fluorescent marking

2004 ◽  
Author(s):  
Matthew B. Hart ◽  
Horn-Bond Lin ◽  
Jason Deich ◽  
Alan L. Huston ◽  
Jay D. Eversole ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Biological Particles

Nanoindentationin silicoof Biological Particles

2017 ◽  
pp. 393-428
Author(s):  
Olga Kononova ◽  
Kenneth A. Marx ◽  
Valeri Barsegov
Keyword(s):  
Biological Particles

Real-time measurement of fluorescence spectra from single airborne biological particles

1999 ◽  
Vol 3 (4-5) ◽  
pp. 221-239 ◽  
Author(s):  
Steven C. Hill ◽  
Ronald G. Pinnick ◽  
Stanley Niles ◽  
Yong-Le Pan ◽  
Stephen Holler ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Spectra ◽  
Time Measurement ◽  
Biological Particles ◽  
Real Time Measurement

scholarly journals Ice nucleating particles at a coastal marine boundary layer site: correlations with aerosol type and meteorological conditions

2015 ◽  
Vol 15 (12) ◽  
pp. 16273-16323 ◽  
Author(s):  
R. H. Mason ◽  
M. Si ◽  
J. Li ◽  
C. Chou ◽  
R. Dickie ◽  
...  
Keyword(s):  
Size Distribution ◽  
Marine Boundary Layer ◽  
Aerosol Particles ◽  
Meteorological Conditions ◽  
Marine Aerosols ◽  
Coastal Site ◽  
Carbon Particles ◽  
Biological Particles ◽  
Ice Nuclei ◽  
Coastal Marine

Abstract. Information on what aerosol particle types are the major sources of ice nucleating particles (INPs) in the atmosphere is needed for climate predictions. To determine which aerosol particles are the major sources of immersion-mode INPs at a coastal site in Western Canada, we investigated correlations between INP number concentrations and both concentrations of different atmospheric particles and meteorological conditions. We show that INP number concentrations are strongly correlated with the number concentrations of fluorescent bioparticles between −15 and −25 °C, and that the size distribution of INPs is most consistent with the size distribution of fluorescent bioparticles. We conclude that biological particles were likely the major source of ice nuclei at freezing temperatures between −15 and −25 °C at this site for the time period studied. At −30 °C, INP number concentrations are also well correlated with number concentrations of the total aerosol particles ≥ 0.5 μm, suggesting that non-biological particles may have an important contribution to the population of INPs active at this temperature. As we found that black carbon particles were unlikely to be a major source of ice nuclei during this study, these non-biological INPs may include mineral dust. Furthermore, correlations involving tracers of marine aerosols and marine biological activity indicate that the majority of INPs measured at the coastal site likely originated from terrestrial rather than marine sources. Finally, six existing empirical parameterizations of ice nucleation were tested to determine if they accurately predict the measured INP number concentrations. We found that none of the parameterizations selected are capable of predicting INP number concentrations with high accuracy over the entire temperature range investigated.

scholarly journals Material- and Size-selective Separation Mechanism of Micro Particles in Frequency-modulated Dielectrophoretic Particle Chromatography

2021 ◽  
Author(s):  
Jasper Giesler ◽  
Laura Weirauch ◽  
Jorg Thöming ◽  
Michael Baune ◽  
Georg R. Pesch
Keyword(s):  
Surface Functionalization ◽  
Selective Separation ◽  
Separation Mechanism ◽  
Separation Techniques ◽  
Retention Mechanisms ◽  
Biological Particles ◽  
Micro Particles ◽  
Good Agreement

Abstract Separation of (biological) particles (<< 10 µm) according to size or other properties is an ongoing challenge in a variety of technical relevant fields. Dielectrophoresis is one method to separate particles according to a diversity of properties, and within the last decades a pool of dielectrophoretic separation techniques has been developed. However, many of them either suffer selectivity or throughput. We use simulation and experiments to investigate retention mechanisms in a novel DEP scheme, namely, frequency-modulated DEP. Results from experiments and simulation show a good agreement for the separation of binary PS particles mixtures with respect to size and more importantly, for the challenging task of separating equally sized microparticles according to surface functionalization alone. The separation with respect to size was performed using 2 µm and 3 µm sized particles, whereas separation with respect to surface functionalization was performed with 2 µm particles. The results from this study can be used to solve challenging separation tasks, for example to separate particles with distributed properties.

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