Coarse and Fine: A New Method for Gender Classification in the Wild

2017 ◽  
pp. 162-171
Author(s):  
Qianbo Jiang ◽  
Li Shao ◽  
Zhengxi Liu ◽  
Qijun Zhao
Keyword(s):  
New Method ◽  
Gender Classification ◽  
In The Wild

scholarly journals Multi-scale score level fusion of local descriptors for gender classification in the wild

2016 ◽  
Vol 76 (4) ◽  
pp. 4695-4711 ◽  
Author(s):  
M. Castrillón-Santana ◽  
J. Lorenzo-Navarro ◽  
E. Ramón-Balmaseda
Keyword(s):  
Scale Score ◽  
Gender Classification ◽  
Score Level Fusion ◽  
Local Descriptors ◽  
Multi Scale ◽  
In The Wild ◽  
Level Fusion

scholarly journals Descriptors and regions of interest fusion for in- and cross-database gender classification in the wild

2017 ◽  
Vol 57 ◽  
pp. 15-24 ◽  
Author(s):  
M. Castrillón-Santana ◽  
J. Lorenzo-Navarro ◽  
E. Ramón-Balmaseda
Keyword(s):  
Regions Of Interest ◽  
Gender Classification ◽  
In The Wild

On using periocular biometric for gender classification in the wild

2016 ◽  
Vol 82 ◽  
pp. 181-189 ◽  
Author(s):  
Modesto Castrillón-Santana ◽  
Javier Lorenzo-Navarro ◽  
Enrique Ramón-Balmaseda
Keyword(s):  
Gender Classification ◽  
In The Wild

scholarly journals A new method for functional analysis of plastid EMBRYO-DEFECTIVEPPR genes by efficiently constructing cosuppression lines in Arabidopsis

2020 ◽  
Author(s):  
Jingli Chen ◽  
Jirong Huang ◽  
Weihua Huang
Keyword(s):  
Functional Analysis ◽  
Higher Plants ◽  
New Method ◽  
Pcr Analysis ◽  
Transcriptional Gene Silencing ◽  
Transcriptional Level ◽  
Pentatricopeptide Repeat ◽  
Endogenous Gene ◽  
Post Transcriptional Gene Silencing ◽  
In The Wild

Abstract Background: Pentatricopeptide-repeat proteins (PPRs) characterized by tandem arrays of a degenerate 35-amino-acid repeat (PPR motif) can bind a single strand RNA and regulate organell gene expression at the post-transcriptional level, including RNA cleavage, splicing, editing and stability etc. PPRs are conserved in all eukaryotes and extremely expanded in higher plants. Many knockout mutants of PPR genes are embryonic lethal. These genes are named EMB PPRs and their functional analysis is particularly difficult.Results: Here, we report a new method for functional analysis of plastid EMB PPRs by efficiently constructing their cosuppression lines in Arabidopsis. When we overexpressed a mutated full length or fragments lacking one or several PPR repeats of EMB PPRs, such as EMB2279, EMB2654 and EMB976 in the wild-type (WT) background, a large portion of T1 plants displayed chlorosis phenotypes, which is similar to those of their knockdown mutants. RT-PCR analysis showed that the exogenous transgene was overexpressed, while the corresponding endogenous mRNA was significantly downregulated in the transgenic lines. However, when these constructs were overexpressed in the PTGS (Post transcriptional Gene Silencing) deficient mutant, rdr6 (rna dependent rna polymerase 6), none of the T1 plants displayed chlorosis phenotypes. These results indicate that the chlorosis phenotype results from post transcriptional silencing of the corresponding endogenous gene (also known as sense cosuppression). Conclusions: Overexpression of an appropriately truncated PPR CDS in WT leads to a high frequency of cosuppression lines with apparent mutant phenotype, and this method can be employed to study the unknown function of EMB PPR genes. By this method, our preliminary data showed that EMB976 is required for splicing of plastid clpP1 intron 2 and ycf3 intron 1.

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