Cardiac Repair by Embryonic Stem-Derived Cells

2008 ◽  
pp. 73-100
Author(s):  
M. Rubart ◽  
L. J. Field
Keyword(s):  
Embryonic Stem ◽  
Cardiac Repair

Abstract 1471: Development and Characterization of Force Generating Human Engineered Heart Tissue from Embryonic Stem Cells

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Christina Rogge ◽  
Michael Didié ◽  
Erich Wettwer ◽  
Ursula Ravens ◽  
Ralph Graichen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Heart Tissue ◽  
Cardiac Repair ◽  
Embryoid Bodies ◽  
Laser Scanning Microscopy ◽  
Neonatal Rat ◽  
Rat Cardiomyocytes ◽  
Engineered Heart Tissue

Engineered Heart Tissue (EHT) from neonatal rat cardiomyocytes has been used successfully as in vitro model and in cardiac repair. Here, we hypothesized that human embryonic stem cells (hESC) can be used to generate EHT with properties of native myocardium. Methods: hESC (hES3-ENVY) were differentiated in embryoid bodies, enzymatically dispersed, and subjected to EHT-generation in circular casting molds (1.5x10 6 cells, 0.4 mg collagen, 10% Matrigel/EHT; inner/outer diameter - 2/4 mm). Contractile function was assessed 10 days after casting under isometric conditions (37°C, 1.5 Hz, Tyrode’s solution). Action potentials (AP) were recorded in spontaneously contracting EHTs with intracellular electrodes (37°C, Tyrode’s solution). Calcium gradients were assessed by confocal laser scanning microscopy (CLSM) after rhod-2 loading. EHT-morphology was examined by CLSM and electron microscopy (EM). Results: hESC-EHTs contracted synchronously and spontaneously at 1.1±0.1 Hz (n=3). Increasing concentrations of extracellular calcium (0.2–2.4 mM) enhanced force of contraction from 53±8 to 199±22 μN (n=8, p<0.05; EC 50 : 0.8±0.04 mM). Isoprenaline (1 μM) at 0.4 mM calcium increased twitch tension from 61±7 to 108±15 μN (n=8, p<0.05) and shortened relaxation time from 111±6 to 87±4 ms (n=3, p<0.05). Cardiomyocytes within EHTs formed a functional syncytium composed of predominantly oriented muscle strands with a high degree of sarcomere differentiation (CLSM, EM). Cell-cell contacts through adherens junctions were identified by EM. Synchronous calcium gradient spread in spontaneously contracting EHTs indicated electrical coupling of individual cells within the multicellular constructs. AP recordings identified pacemaker cells (spontaneous diastolic depolarization) and cells with a flat phase 4 of the AP (working myocardium-like cells). Pharmacological studies demonstrated the presence and functional relevance of I Na (10–30 μM flecainide), I Ca (1 μM nisoldipine), and I Kr (1–5 μM E4031). Conclusion: Human force-generating EHT with functional and morphological properties of native myocardium can be generated. Ultimately, hESC-EHTs may constitute a model system for substance screening and could further be utilized in cardiac repair.

scholarly journals Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac Repair

2015 ◽  
Vol 5 (5) ◽  
pp. 753-762 ◽  
Author(s):  
Sarah Fernandes ◽  
James J.H. Chong ◽  
Sharon L. Paige ◽  
Mineo Iwata ◽  
Beverly Torok-Storb ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Mononuclear Cells ◽  
Embryonic Stem ◽  
Cardiac Repair ◽  
Bone Marrow Mononuclear Cells

scholarly journals Human embryonic stem cells and cardiac repair

2009 ◽  
Vol 23 (1) ◽  
pp. 53-68 ◽  
Author(s):  
Wei-Zhong Zhu ◽  
Kip D. Hauch ◽  
Chunhui Xu ◽  
Michael A. Laflamme
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cardiac Repair

scholarly journals Human Embryonic Stem Cell–Derived Cardiomyocytes and Cardiac Repair in Rodents

2008 ◽  
Vol 102 (9) ◽  
pp. 1008-1010 ◽  
Author(s):  
Linda W. van Laake ◽  
Robert Passier ◽  
Pieter A. Doevendans ◽  
Christine L. Mummery
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cardiac Repair

scholarly journals Use of human embryonic stem cell derived-mesenchymal cells for cardiac repair

2011 ◽  
Vol 109 (1) ◽  
pp. 274-283 ◽  
Author(s):  
David L. Simpson ◽  
Nolan L. Boyd ◽  
Sunjay Kaushal ◽  
Steve L. Stice ◽  
Samuel C. Dudley
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mesenchymal Cells ◽  
Cardiac Repair

scholarly journals Cell Therapy with Embryonic Stem Cell-Derived Cardiomyocytes Encapsulated in Injectable Nanomatrix Gel Enhances Cell Engraftment and Promotes Cardiac Repair

ACS Nano ◽  
2014 ◽  
Vol 8 (10) ◽  
pp. 10815-10825 ◽  
Author(s):  
Kiwon Ban ◽  
Hun-Jun Park ◽  
Sangsung Kim ◽  
Adinarayana Andukuri ◽  
Kyu-Won Cho ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Therapy ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Cardiac Repair ◽  
Cell Engraftment

Human Embryonic Stem Cells vs Human Induced Pluripotent Stem Cells for Cardiac Repair

2014 ◽  
Vol 30 (11) ◽  
pp. 1279-1287 ◽  
Author(s):  
Lili Barad ◽  
Revital Schick ◽  
Naama Zeevi-Levin ◽  
Joseph Itskovitz-Eldor ◽  
Ofer Binah
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Pluripotent Stem Cells ◽  
Embryonic Stem ◽  
Cardiac Repair ◽  
Induced Pluripotent

Therapeutic angiogenesis by transplantation of human embryonic stem cell-derived CD133+endothelial progenitor cells for cardiac repair

2010 ◽  
Vol 5 (2) ◽  
pp. 231-244 ◽  
Author(s):  
Abdul Jalil Rufaihah ◽  
Husnain Khawaja Haider ◽  
Boon Chin Heng ◽  
Lei Ye ◽  
Ru San Tan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Therapeutic Angiogenesis ◽  
Cardiac Repair ◽  
Endothelial Progenitor
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