Time-Resolved FTIR Difference Spectroscopy of Photosynthetic Bacterial Reaction Centers

1992 ◽  
pp. 79-82 ◽  
Author(s):  
D. L. Thibodeau ◽  
E. Nabedryk ◽  
R. Hienerwadel ◽  
F. Lenz ◽  
W. Mäntele ◽  
...  
Keyword(s):  
Reaction Centers ◽  
Difference Spectroscopy ◽  
Time Resolved ◽  
Bacterial Reaction Centers ◽  
Ftir Difference Spectroscopy

scholarly journals Probing the primary quinone environment in photosynthetic bacterial reaction centers by light-induced FTIR difference spectroscopy

FEBS Letters ◽  
1991 ◽  
Vol 278 (2) ◽  
pp. 257-260 ◽  
Author(s):  
J. Breton ◽  
D.L. Thibodeau ◽  
C. Berthomieu ◽  
W. Mäntele ◽  
A. Vermèglio ◽  
...  
Keyword(s):  
Reaction Centers ◽  
Difference Spectroscopy ◽  
Bacterial Reaction Centers ◽  
Ftir Difference Spectroscopy

scholarly journals Rapid-scan FTIR difference spectroscopy applied to ubiquinone reduction in photosynthetic reaction centers: Role of redox mediators

2010 ◽  
Vol 24 (1-2) ◽  
pp. 79-87 ◽  
Author(s):  
Alberto Mezzetti
Keyword(s):  
Reaction Centers ◽  
Photosynthetic Reaction Centers ◽  
Difference Spectroscopy ◽  
Redox Mediators ◽  
Time Resolved ◽  
Rapid Scan ◽  
Primary Donor ◽  
Ftir Difference Spectroscopy ◽  
Kinetics Of

Rapid-scan FTIR difference spectroscopy was used to investigate light-induced reduction of the ubiquinone QB, and the oxidation kinetics of QB–and QH2by external mediators in isolated reaction centers (RCs) fromR. sphaeroides. As redox mediators, a Ferrocyanide/N,N,Nʹ,Nʹ-tetramethyl-p-phenylenediamine (TMPD) mixture and an Ascorbate/2,3,5,6-tetramethyl-p-phenylenediamine (DAD) mixture are compared.Results show that TMPDredrapidly reduces the photoproduced P870+primary donor. The process is fast enough to record rapid-scan FTIR spectra devoid of P870+bands down to 260 K. Results show also that TMPDoxoxidises both QB–and QH2faster than DADox. In particular, QB–is oxidised faster than QH2at all temperatures studied.Results are discussed in the framework of time-resolved infrared studies onR. sphaeroidesRCs, showing advantages/drawbacks of the proposed experimental approach.

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