Isolation and characterization of the Rhizobium meliloti Tn5-mutants with impaired nodulation competitiveness

1994 ◽  
Vol 167 (2) ◽  
pp. 267-274 ◽  
Author(s):  
O. P. Onishchuk ◽  
L. A. Sharypova ◽  
B. V. Simarov
Keyword(s):  
Rhizobium Meliloti ◽  
Isolation And Characterization ◽  
Nodulation Competitiveness ◽  
Tn5 Mutants

scholarly journals Isolation and characterization of Rhizobium meliloti Tn5 mutants showing enhanced symbiotic effectiveness

Microbiology ◽  
1994 ◽  
Vol 140 (3) ◽  
pp. 463-470 ◽  
Author(s):  
L. A. Sharypova ◽  
O. P. Onishchuk ◽  
O. N. Chesnokova ◽  
J. G. Fomina-Eshchenko ◽  
B. V. Simarov
Keyword(s):  
Rhizobium Meliloti ◽  
Symbiotic Effectiveness ◽  
Isolation And Characterization ◽  
Tn5 Mutants

scholarly journals Salt-tolerance genes involved in cation efflux and osmoregulation ofSinorhizobium frediiRT19 detected by isolation and characterization of Tn5 mutants

2004 ◽  
Vol 239 (1) ◽  
pp. 139-146 ◽  
Author(s):  
Ju Quan Jiang ◽  
Wei Wei ◽  
Bing Hai Du ◽  
Xiao Hong Li ◽  
Lei Wang ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Isolation And Characterization ◽  
Tn5 Mutants

scholarly journals Isolation and Characterization of Rhizobium meliloti Isolated from Rhizosphere Soil and Roots of Fenugreek from Different Locations

2018 ◽  
Vol 7 (06) ◽  
pp. 1460-1467
Author(s):  
Satyadev Prajapati ◽  
M. S. Dadke ◽  
S. Surekha ◽  
S. Godika ◽  
V. Prasanna Krishna
Keyword(s):  
Rhizosphere Soil ◽  
Rhizobium Meliloti ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of the recA gene of Rhizobium meliloti.

1983 ◽  
Vol 155 (1) ◽  
pp. 311-316 ◽  
Author(s):  
M Better ◽  
D R Helinski
Keyword(s):  
Rhizobium Meliloti ◽  
Reca Gene ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of an R-prime plasmid from Rhizobium meliloti.

1980 ◽  
Vol 141 (1) ◽  
pp. 121-128 ◽  
Author(s):  
G B Kiss ◽  
K Dobo ◽  
I Dusha ◽  
A Breznovits ◽  
L Orosz ◽  
...  
Keyword(s):  
Rhizobium Meliloti ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of the constitutive acyl carrier protein from Rhizobium meliloti.

1990 ◽  
Vol 172 (9) ◽  
pp. 5440-5444 ◽  
Author(s):  
M W Platt ◽  
K J Miller ◽  
W S Lane ◽  
E P Kennedy
Keyword(s):  
Rhizobium Meliloti ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Isolation And Characterization

Isolation and characterization of mutants of Rhizobium meliloti unable to synthesize poly-β-hydroxybutyrate

1994 ◽  
Vol 40 (10) ◽  
pp. 823-829 ◽  
Author(s):  
Silvana Povolo ◽  
Riccardo Tombolini ◽  
Antonella Morea ◽  
Alistair J. Anderson ◽  
Sergio Casella ◽  
...  
Keyword(s):  
Rhizobium Meliloti ◽  
Tn5 Transposon ◽  
Transposon Insertion ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Genome Level ◽  
Acetylene Reducing Activity ◽  
Phb Synthase ◽  
Dna Profiles

The isolation and characterization of four mutants of Rhizobium meliloti unable to synthesize poly-β-hydroxybutyrate (PHB) are reported. The mutants were independently obtained via Tn5 transposon mutagenesis and exhibited physiological and cytomorphological characteristics similar to those of the parental strain, as well as overlapping DNA profiles. These were assessed at both the plasmid and total genome level, using for the latter the sensitive technique of pulsed-field gel electrophoresis in a contour-clamped homogeneous electric field. With respect to the parental PHB+ strain, the loss of PHB-synthesizing ability in the four mutants was demonstrated by gas chromatography, transmission electron microscopy, and enzymatic tests. Localization studies of Tn5 insertion showed that the PHB− phenotype had, in all mutants, a transposon insertion in the same region, although not in the same position. The symbiotic traits (nodule-inducing ability on Medicago sativa and acetylene-reducing activity of nodules) of the mutants did not differ significantly from those of the parental R. meliloti.Key words: Rhizobium meliloti, poly-β-hydroxybutyrate (PHB), PHB synthase.

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