Heterogeneous photosystem 2 activity in isolated spinach chloroplasts

1990 ◽  
Vol 24 (3) ◽  
pp. 209-220 ◽  
Author(s):  
John Sinclair ◽  
Sandra M. Spence
Keyword(s):  
Photosystem 2 ◽  
Spinach Chloroplasts ◽  
Photosystem 2 Activity

Changes in photosystem 2 activity associated with plant tolerance to lead

1981 ◽  
Vol 21 (3) ◽  
pp. 269-274 ◽  
Author(s):  
J.R. Homer ◽  
R. Cotton ◽  
E.H. Evans
Keyword(s):  
Photosystem 2 ◽  
Plant Tolerance ◽  
Photosystem 2 Activity

Coloured Components of Chloroplast Membranes as Intrinsic Membrane Probes for Monitoring the Development of Heat Injury in Intact Tissues

1979 ◽  
Vol 6 (1) ◽  
pp. 121 ◽  
Author(s):  
RM Smillie
Keyword(s):  
Chlorophyll Fluorescence ◽  
Photosystem 2 ◽  
Leaf Tissue ◽  
Proton Pumping ◽  
Cytochrome F ◽  
Heat Sensitivity ◽  
Heat Injury ◽  
Chloroplast Membrane ◽  
Photosystem 2 Activity ◽  
Membrane Probes

Assays for heat injury in intact leaves were investigated by heating leaf tissue at a rate of 1°C per minute and recording changes in chlorophyll fluorescence; chlorophyll absorbance; light-induced absorbance change at 518 nm of the bulk chloroplast pigments (chlorophylls and carotenoids); and photooxidation of chloroplast cytochrome-554 (cytochrome f ). Leaves of the golden passionfruit (Passiflora edulis forma flavicarpa were used as the experimental material. Heat injury to the chloroplast thylakoid membranes began above 35°C as evidenced by abnormal changes in chlorophyll fluorescence and absorbance and inactivation of chloroplast membrane activities. Chlorophyll fluorescence increased and chlorophyll absorbance decreased above 35°C and the increase and decrease, respectively, were greatest at 52°C. The measurements made on intact leaf tissue together with assays of photosystem 2 activity in isolated chloroplasts indicated the relative thermostabilities of several chloroplast membrane activities. Inactivation of photosystem 2 activity linked to oxygen evolution appeared to be associated with membrane changes occurring between 35 and 44°C. The 518-nm absorbance changes indicated that energy-dependent proton pumping and associated reactions were inactivated between 44 and 48°C. Photosystem-1-mediated photooxidation of cytochrome-554 became inactivated between 48 and 52°C. These studies demonstrate that chlorophylls, carotenoids and cytochrome f may be used as intrinsic membrane probes for monitoring the development of heat injury in the chloroplast membrane system. The absorbance and fluorescence changes that these compounds undergo upon heating provide a basis for reliable and rapid methods applicable to intact tissue for determining heat sensitivity in green plants.

Chromium increases photosystem 2 activity in Brassica juncea

2009 ◽  
Vol 53 (1) ◽  
pp. 100-104 ◽  
Author(s):  
S. Gupta ◽  
S. Srivastava ◽  
P. Saradhi
Keyword(s):  
Brassica Juncea ◽  
Photosystem 2 ◽  
Photosystem 2 Activity

Photosystem 2 activity of Citrus volkameriana (L.) leaves as affected by Mn nutrition and irradiance

2007 ◽  
Vol 45 (2) ◽  
pp. 208-213 ◽  
Author(s):  
I. E. Papadakis ◽  
A. Giannakoula ◽  
C. P. Antonopoulou ◽  
M. Moustakas ◽  
E. Avramaki ◽  
...  
Keyword(s):  
Photosystem 2 ◽  
Photosystem 2 Activity ◽  
Citrus Volkameriana

Impairment of photosystem 2 activity at the level of secondary quinone electron acceptor in chloroplasts treated with cobalt, nickel and zinc ions

1989 ◽  
Vol 76 (3) ◽  
pp. 386-390 ◽  
Author(s):  
Narendranath Mohanty ◽  
Imre Vass ◽  
Sándor Demeter
Keyword(s):  
Electron Acceptor ◽  
Photosystem 2 ◽  
Zinc Ions ◽  
Cobalt Nickel ◽  
Photosystem 2 Activity
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