Effect of genotype on somatic embryogenesis from immature cotyledons of soybean

1989 ◽  
Vol 16 (1) ◽  
pp. 15-21 ◽  
Author(s):  
W. A. Parrott ◽  
E. G. Williams ◽  
D. F. Hildebrand ◽  
G. B. Collins
Keyword(s):  
Somatic Embryogenesis ◽  
Immature Cotyledons

Development of a Superficial Meristem During Somatic Embryogenesis from Immature Cotyledons of Soybean (Glycine max L.)

1988 ◽  
Vol 62 (5) ◽  
pp. 513-519 ◽  
Author(s):  
A. HEPHER ◽  
M. E. BOULTER ◽  
N. HARRIS ◽  
R. S. NELSON
Keyword(s):  
Somatic Embryogenesis ◽  
Glycine Max ◽  
Immature Cotyledons ◽  
Glycine Max L

Somatic embryogenesis from immature cotyledons of an elite Darjeeling tea clone

Plant Science ◽  
1992 ◽  
Vol 84 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Timir Baran Jha ◽  
Sumita Jha ◽  
S.K. Sen
Keyword(s):  
Somatic Embryogenesis ◽  
Immature Cotyledons ◽  
Darjeeling Tea

Plant regeneration via somatic embryogenesis and shoot organogenesis from immature cotyledons of Camellia nitidissima Chi

2013 ◽  
Vol 170 (13) ◽  
pp. 1202-1211 ◽  
Author(s):  
Jinfeng Lü ◽  
Rong Chen ◽  
Muhan Zhang ◽  
Jaime A. Teixeira da Silva ◽  
Guohua Ma
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Shoot Organogenesis ◽  
Immature Cotyledons ◽  
Camellia Nitidissima

scholarly journals 886 PB 460 SOMATIC EMBRYOGENESIS FROM IMMATURE COTYLEDONS OF TEXAS BLUEBONNET

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 560g-560
Author(s):  
Daksha Sankhla ◽  
Tim D. Davis ◽  
N. Sankhla
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Ms Medium ◽  
Embryo Maturation ◽  
Embryo Germination ◽  
Precocious Germination ◽  
Embryogenic Potential ◽  
Conversion Frequency ◽  
Maturation Medium ◽  
Immature Cotyledons

This study was initiated to test the embryogenic potential of immature cotyledons (3-5 mm long) of Texas bluebonnet (Lupinus texensis). The embryo initiation medium consisted of B5 salts and vitamins with 3% sucrose and 22.6 μM 2,4-D alone or in combination with 1-15 μM of various cytokinins. Within 15-20 days, globular embryos were formed on the distal end of the cotyledons. Eventually the entire cotyledon surface was covered by embryo-like structures. Addition of cytokinins to the medium did not increase the percentage of cultures which formed embryos. In fact, addition of thidiazuron severely inhibited embryogenesis. Following transfer to an embryo maturation medium (MS medium with 0.38 μM abscisic acid alone or in combination with benzyladenine or zeatin) for 10-14 days, the embryos were placed in MS medium supplemented with GA (2.9 μM) or glutamine (200 mg/liter) with or without activated charcoal (0.5%) for embryo germination and plantlet development. Most of the embryos exhibited precocious germination and well-developed roots but failed to produce normal shoots. Therefore, additional work is needed to improve embryo conversion frequency.

scholarly journals Somatic embryogenesis from immature cotyledons of soybean (Glycine max (L.) Merr.): ontogeny of somatic embryos

2006 ◽  
Vol 49 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Karla Galvão Bezerra dos Santos ◽  
Jorge Ernesto de Araújo Mariath ◽  
Maria Cecília C. Moço ◽  
Maria Helena Bodanese-Zanettini
Keyword(s):  
Somatic Embryogenesis ◽  
Glycine Max ◽  
Somatic Embryos ◽  
Zygotic Embryos ◽  
Immature Zygotic Embryos ◽  
Immature Cotyledons ◽  
Glycine Max L

Soybean somatic embryos obtained from immature zygotic embryos were histologically analyzed in order to determine possible ontogenetic routes followed by these embryos. The proembryo stage and globular, heart-shaped, torpedo and cotyledonary embryo stages were found, closely resembling the ontogeny of zygotic embryos. However, the absence of a characteristic suspensor as well as the delay in the establishment of inner organization were the main differences between these two embryogenic processes.

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