Protoplast-to-plant regeneration in cotton (Gossypium hirsutum L. cv. Coker 312) using feeder layers

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Vol 13-13 (3-4) ◽  
Author(s):  
Marie-Christine Peeters ◽  
Katleen Willems ◽  
Rony Swennen
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Vol 5 (3) ◽  
pp. 178-181 ◽  
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R. C. Shoemaker ◽  
L. J. Couche ◽  
D. W. Galbraith

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Vol 49 (3) ◽  
pp. 193-196 ◽  
Author(s):  
BAO-HONG ZHANG ◽  
RONG FENG ◽  
FANG LIU ◽  
DA-YUN ZHOU ◽  
QING-LIAN WANG

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Vol 3 (3) ◽  
pp. 279-283 ◽  
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Jingyan Sun ◽  
Weiming Li ◽  
Hanshuang Zhang ◽  
Junli Zhao ◽  
Xiaolin Yin ◽  
...  

Crop Science ◽  
2001 ◽  
Vol 41 (4) ◽  
pp. 1235-1240 ◽  
Author(s):  
Hamidou F. Sakhanokho ◽  
Allan Zipf ◽  
Kanniah Rajasekaran ◽  
Sukumar Saha ◽  
Govind C. Sharma

1998 ◽  
Vol 17 (4) ◽  
pp. 273-278 ◽  
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C. G. A. Maier ◽  
K. D. Chapman

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pp. 1771-1774 ◽  
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Shengwei Zhu ◽  
Jingsan Sun

2014 ◽  
Vol 8 (2) ◽  
pp. 90-94
Author(s):  
Hussein Abdullah Ahmed ◽  
Mortaza Hajyzadeh ◽  
Surendra Barpete ◽  
Sebahattin Ozcan

In plant tissue culture studies, obtaining new plantlets from different parts of plant is a very important feature with direct or indirect ways of regeneration. The plant show different regeneration capacities from species to species. In this present study, in-vitro direct shoots development from embryonic axis of cotton (Gossypium hirsutum L. cv. Lashata) were comparatively studied. Embryonic axis, embryonic hypocotyl and plumule obtained from five days old in-vitro grown seeds. All explants were cultured on MS media supplemented with 1- 4 mg/l Benzyl amino purine (BAP) + 0.1 mg/l Naphthalene acetic acid (NAA) and 3 mg/l activated charcoal. Cultured explants were kept at growth chamber with photoperiod for 16 hours light and 8 hours dark, at 25oC. Reproducible in-vitro plant regeneration was obtained from embryonic axis when cultured onto MS medium supplemented 1 mg/l BAP + 0.1 mg/l NAA and 3 g/l Activated charcoal. Shoot induction was to be noted 53.3%, 36.7% and 12.3 in embryonic axis, plumule and embryonic hypocotyl respectively. All these shoots were capable of rooting on MS medium supplemented with 0.1 mg/l NAA, and establishing in soil 3-4 weeks.


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