Restriction endonuclease analysis of chloroplast DNA in interspecies somatic Hybrids of Petunia

1982 ◽  
Vol 62 (4) ◽  
pp. 377-383 ◽  
Author(s):  
A. Kumar ◽  
E. C. Cocking ◽  
W. A. Bovenberg ◽  
A. J. Kool
Keyword(s):  
Chloroplast Dna ◽  
Restriction Endonuclease ◽  
Somatic Hybrids ◽  
Restriction Endonuclease Analysis ◽  
Endonuclease Analysis

scholarly journals Restriction endonuclease analysis of chloroplast DNA from A-genome diploid species of rice.

1988 ◽  
Vol 63 (6) ◽  
pp. 523-536 ◽  
Author(s):  
Takashige ISHII ◽  
Toru TERACHI ◽  
Koichiro TSUNEWAKI
Keyword(s):  
Chloroplast Dna ◽  
Restriction Endonuclease ◽  
Diploid Species ◽  
Restriction Endonuclease Analysis ◽  
A Genome ◽  
Endonuclease Analysis

Chloroplast genome differentiation in Asian cultivated rice

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 818-826 ◽  
Author(s):  
Takashige Ishii ◽  
Koichiro Tsunewaki
Keyword(s):  
Oryza Sativa ◽  
Chloroplast Dna ◽  
Chloroplast Genome ◽  
Restriction Endonuclease ◽  
Genetic Relationships ◽  
Restriction Endonuclease Analysis ◽  
Asian Countries ◽  
Genome Differentiation ◽  
Chloroplast Genomes ◽  
Endonuclease Analysis

Restriction endonuclease analysis of chloroplast DNA was carried out to clarify the chloroplast genome differentiation between Asian varieties of Oryza sativa. Based on the restriction fragment patterns obtained with six endonucleases, i.e., EcoRI, HindIII, PstI, PvuII, SmaI, and XhoI, chloroplast genomes of 68 local varieties from 15 Asian countries could be classified into five types (types 1, 3, 10, 11, and 12). Among these types, four length mutations and two base substitutions were found; these changes were located on the PstI physical map of rice chloroplast DNA. In a dendrogram showing genetic relationships among five chloroplast genomes, they are mainly divided into two groups, which we have named as the Japonica (types 1, 11, and 12) and Indica (types 3 and 10) chloroplast genome groups. Both groups are distributed widely in Asian countries. The Japonica isozyme group classified by J.C. Glaszmann (1985. Rice Genetics. International Rice Research Institute, Manila, Philippines, pp. 83–90) carries only the Japonica chloroplast genome, whereas the Indica isozyme group contains both Japonica and Indica chloroplast genomes.Key words: Oryza sativa, Asian varieties, chloroplast DNA, restriction endonuclease analysis, chloroplast genome differentiation.

Plasmon diversity in Triticum and Aegilops and its implication in wheat evolution

Genome ◽  
1989 ◽  
Vol 31 (1) ◽  
pp. 143-154 ◽  
Author(s):  
Koichiro Tsunewaki
Keyword(s):  
Phylogenetic Tree ◽  
Chloroplast Dna ◽  
Restriction Endonuclease ◽  
Fragment Length Polymorphism ◽  
Restriction Endonuclease Analysis ◽  
Polymorphism Analysis ◽  
Aegilops Species ◽  
Maternal Lineage ◽  
Restriction Fragment Length ◽  
Endonuclease Analysis

Recent results on the plastome, chondriome, and plasmon diversity among Triticum (wheat) and Aegilops species have been reviewed. As to the plastome diversity, 17 different chloroplast DNA types were found among 32 species (42 accessions in total) by restriction endonuclease analysis of chloroplast DNA. Referring to the physical map of common wheat chloroplast DNA, 46 plastome mutations have been identified, and based on the number of the mutations detected between every pair of the plastomes, their phylogenetic tree has been constructed. Complete base sequence of five rbcL genes encoding rubisco large subunit, which were isolated from five different plastomes, clarified the amino acid differences that are responsible for the electrophoretic difference between the H- and L-type large subunits. A phylogenetic tree was constructed for these genes, including their flanking regions, based on the number of mutations observed between them. This tree closely resembled the phylogenetic tree of their carrier plastomes, except in one minor point. As to the chondriome diversity, both the restriction endonuclease analysis and restriction fragment length polymorphism analysis of mitochondrial DNA demonstrated higher variability of mitochondrial DNA than chloroplast DNA, proving its usefulness in elucidation of the plasmon differentiation between closely related species or even within a species. Plasmon diversity revealed by comparing phenotypes of eu- and allo-plasmic lines of emmer, timopheevi, or common wheat indicated that all characters observed are affected by alien plasmons, among which male sterility, pistillody, haploid parthenogenesis, prematurity sprouting, variegation, and growth depression are specifically expressed by different genome–plasmon interactions. Nuclear genes responsible for their expression are determined in limited cases. As to the intraspecific variation of the plasmon, Aegilops speltoides showed large intraspecific variation, whereas two wild tetraploid wheats, Triticum dicoccoides and Triticum araraticum, revealed little variation. Aegilops triuncialis was dimorphic and proved to have originated from the reciprocal crosses between Aegilops caudata and Aegilops umbellulata. Combining all the data available, the plasmon in Triticum and Aegilops species has been classified into 16 types. Plasmon diversity decreases with the increase in the ploidy. Among the diploids, differentiation of the plasmon is paralleled with that of the nuclear genome. Origin of the plasmon of most polyploid species has been determined definitely, and for the remaining, their plasmon origin could be postulated. Thus, the maternal lineage of the species in these two genera has been clarified to a great extent.Key words: Triticum, Aegilops, plasmon, plastome, chondriome, rbcL gene, variation, phylogeny, maternal lineage, restriction endonuclease analysis, restriction fragment length polymorphism analysis, base sequencing, alloplasmic wheat, phenotypic effect.

scholarly journals Phylogenetic relationship between the tuberous Solanum species as revealed by restriction endonuclease analysis of chloroplast DNA.

1984 ◽  
Vol 59 (4) ◽  
pp. 349-369 ◽  
Author(s):  
Kazuyoshi HOSAKA ◽  
Yasunari OGIHARA ◽  
Motokazu MATSUBAYASHI ◽  
Koichiro TSUNEWAKI
Keyword(s):  
Chloroplast Dna ◽  
Restriction Endonuclease ◽  
Phylogenetic Relationship ◽  
Restriction Endonuclease Analysis ◽  
Solanum Species ◽  
Endonuclease Analysis

scholarly journals Restriction endonuclease analysis of chloroplast DNA from cultivated rice species, Oryza sativa and O. glaberrima.

1986 ◽  
Vol 61 (6) ◽  
pp. 537-541 ◽  
Author(s):  
Takashige ISHII ◽  
Toru TERACHI ◽  
Koichiro TSUNEWAKI
Keyword(s):  
Oryza Sativa ◽  
Chloroplast Dna ◽  
Restriction Endonuclease ◽  
Restriction Endonuclease Analysis ◽  
Cultivated Rice ◽  
Endonuclease Analysis

Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids

1982 ◽  
Vol 186 (4) ◽  
pp. 453-459 ◽  
Author(s):  
Barbara Schiller ◽  
R. G. Herrmann ◽  
G. Melchers
Keyword(s):  
Restriction Endonuclease ◽  
Somatic Hybrids ◽  
Plastid Dna ◽  
Restriction Endonuclease Analysis ◽  
Endonuclease Analysis
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