Regulation of NAD metabolism in Salmonella typhimurium: Genetic analysis and cloning of the nadR repressor locus

1987 ◽  
Vol 208 (1-2) ◽  
pp. 279-287 ◽  
Author(s):  
John W. Foster ◽  
Elizabeth A. Holley-Guthrie ◽  
Felicity Warren

Genetics ◽  
1989 ◽  
Vol 123 (4) ◽  
pp. 625-633 ◽  
Author(s):  
M B Schmid ◽  
N Kapur ◽  
D R Isaacson ◽  
P Lindroos ◽  
C Sharpe

Abstract We have isolated 440 mutants of Salmonella typhimurium that show temperature-sensitive growth on complex medium at 44 degrees. Approximately 16% of the mutations in these strains have been mapped to 17 chromosomal locations; two of these chromosomal locations seem to include several essential genes. Genetic analysis of the mutations suggests that the collection saturates the genes readily mutable to a ts lethal phenotype in S. typhimurium. Physiological characteristics of the ts lethal mutants were tested: 6% of the mutants can grow at high temperature under anaerobic conditions, 17% can grow when the medium includes 0.5 M KCl, and 9% of the mutants die after a 2-hr incubation at the nonpermissive temperature. Most ts lethal mutations in this collection probably affect genes required for growth at all temperatures (not merely during high temperature growth) since Tn10 insertions that cause a temperature-sensitive lethal phenotype are rare.





Microbiology ◽  
1987 ◽  
Vol 133 (11) ◽  
pp. 3071-3080 ◽  
Author(s):  
H. Fujita ◽  
S. Yamaguchi ◽  
T. Taira ◽  
T. Hirano ◽  
T. Iino


Genetics ◽  
2007 ◽  
Vol 177 (2) ◽  
pp. 1125-1139 ◽  
Author(s):  
Vanessa Sancho-Shimizu ◽  
Rabia Khan ◽  
Serge Mostowy ◽  
Line Larivière ◽  
Rosalie Wilkinson ◽  
...  


Genetics ◽  
1965 ◽  
Vol 52 (3) ◽  
pp. 643-651
Author(s):  
Yanhon Yan ◽  
M Demerec


Genetics ◽  
1967 ◽  
Vol 56 (2) ◽  
pp. 341-351
Author(s):  
Y Nishioka ◽  
M Demerec ◽  
A Eisenstark


Microbiology ◽  
1984 ◽  
Vol 130 (11) ◽  
pp. 2873-2881 ◽  
Author(s):  
J. W. Foster ◽  
E. A. Holley ◽  
S. Mya


Sign in / Sign up

Export Citation Format

Share Document