Cloning and biochemical characterization of LYS5 gene of Saccharomyces cerevisiae

1988 ◽  
Vol 13 (4) ◽  
pp. 299-304 ◽  
Author(s):  
Christopher W. Borell ◽  
J. K. Bhattacharjee
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Biochemical Characterization ◽  
Lys5 Gene

Genetic and biochemical characterization of Saccharomyces cerevisiae mutants resistant to trifluoroleucine

1997 ◽  
Vol 148 (7) ◽  
pp. 613-623 ◽  
Author(s):  
E. Casalone ◽  
G. Fia ◽  
C. Barberio ◽  
D. Cavalieri ◽  
L. Turbanti ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Biochemical Characterization

scholarly journals Molecular Characterization of Saccharomyces cerevisiae TFIID

2002 ◽  
Vol 22 (16) ◽  
pp. 6000-6013 ◽  
Author(s):  
Steven L. Sanders ◽  
Krassimira A. Garbett ◽  
P. Anthony Weil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Transcription Factor ◽  
Molecular Mass ◽  
Molecular Characterization ◽  
Biochemical Characterization ◽  
Gel Filtration ◽  
Calculated Molecular Mass ◽  
General Transcription Factor

ABSTRACT We previously defined Saccharomyces cerevisiae TFIID as a 15-subunit complex comprised of the TATA binding protein (TBP) and 14 distinct TBP-associated factors (TAFs). In this report we give a detailed biochemical characterization of this general transcription factor. We have shown that yeast TFIID efficiently mediates both basal and activator-dependent transcription in vitro and displays TATA box binding activity that is functionally distinct from that of TBP. Analyses of the stoichiometry of TFIID subunits indicated that several TAFs are present at more than 1 copy per TFIID complex. This conclusion was further supported by coimmunoprecipitation experiments with a systematic family of (pseudo)diploid yeast strains that expressed epitope-tagged and untagged alleles of the genes encoding TFIID subunits. Based on these data, we calculated a native molecular mass for monomeric TFIID. Purified TFIID behaved in a fashion consistent with this calculated molecular mass in both gel filtration and rate-zonal sedimentation experiments. Quite surprisingly, although the TAF subunits of TFIID cofractionated as a single complex, TBP did not comigrate with the TAFs during either gel filtration chromatography or rate-zonal sedimentation, suggesting that TBP has the ability to dynamically associate with the TFIID TAFs. The results of direct biochemical exchange experiments confirmed this hypothesis. Together, our results represent a concise molecular characterization of the general transcription factor TFIID from S. cerevisiae.

Purification and biochemical characterization of recombinant hirudin produced by Saccharomyces cerevisiae

Biochemistry ◽  
1989 ◽  
Vol 28 (7) ◽  
pp. 2941-2949 ◽  
Author(s):  
Nadine Riehl-Bellon ◽  
Dorothee Carvallo ◽  
Michele Acker ◽  
Alain Van Dorsselaer ◽  
Magda Marquet ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Biochemical Characterization ◽  
Recombinant Hirudin

Genetical and biochemical characterization of saccharomyces cerevisiae industrial strains

1993 ◽  
Vol 346 (6-9) ◽  
pp. 868-871 ◽  
Author(s):  
Gotthard Kunze ◽  
Irene Kunze ◽  
Andreas Barner ◽  
Ronald Schulz
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Biochemical Characterization ◽  
Industrial Strains

DNA ligase I from Saccharomyces cerevisiae: physical and biochemical characterization of the CDC9 gene product

Biochemistry ◽  
1992 ◽  
Vol 31 (47) ◽  
pp. 11762-11771 ◽  
Author(s):  
Alan E. Tomkinson ◽  
Nancy J. Tappe ◽  
Errol C. Friedberg
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Product ◽  
Biochemical Characterization ◽  
Dna Ligase ◽  
Dna Ligase I
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