The electrochemical proton potential generated by the sulphur respiration of Wolinella succinogenes

1989 ◽  
Vol 152 (6) ◽  
pp. 600-605 ◽  
Author(s):  
Christiane Wloczyk ◽  
Achim Kr�ger ◽  
Thomas G�bel ◽  
Gabriele Holdt ◽  
Ralf Steudel
Keyword(s):  
Wolinella Succinogenes ◽  
Proton Potential ◽  
Sulphur Respiration

Isolation of the sulphur reductase and reconstitution of the sulphur respiration of Wolinella succinogenes

1988 ◽  
Vol 149 (6) ◽  
pp. 572-579 ◽  
Author(s):  
I. Schr�der ◽  
A. Kr�ger ◽  
J. M. Macy
Keyword(s):  
Wolinella Succinogenes ◽  
Sulphur Respiration

scholarly journals Spectroscopic characterization of a high-potential monohaem cytochrome from Wolinella succinogenes, a nitrate-respiring organism. Redox and spin equilibria studies

1988 ◽  
Vol 177 (3) ◽  
pp. 673-682 ◽  
Author(s):  
Isabel MOURA ◽  
Ming Y. LIU ◽  
Cristina COSTA ◽  
Ming C. LIU ◽  
Govind PAI ◽  
...  
Keyword(s):  
Spectroscopic Characterization ◽  
High Potential ◽  
Wolinella Succinogenes

DMSO respiration by the anaerobic rumen bacterium Wolinella succinogenes

1994 ◽  
Vol 162 (4) ◽  
pp. 277-281 ◽  
Author(s):  
J�rgen Lorenzen ◽  
Swantje Steinwachs ◽  
Gottfried Unden
Keyword(s):  
Rumen Bacterium ◽  
Wolinella Succinogenes

The proton potential in short hydrogen bonds

1983 ◽  
Vol 100 ◽  
pp. 393-402 ◽  
Author(s):  
D. Hadži
Keyword(s):  
Hydrogen Bonds ◽  
Proton Potential ◽  
Short Hydrogen Bonds

Regulation of anaerobic respiratory pathways in Wolinella succinogenes by the presence of electron acceptors

1993 ◽  
Vol 159 (5) ◽  
pp. 477-483 ◽  
Author(s):  
J. P. Lorenzen ◽  
A. Kr�ger ◽  
G. Unden
Keyword(s):  
Electron Acceptors ◽  
Wolinella Succinogenes

Methanogenesis from Acetate by Methanosarcina barkeri: Catalysis of Acetate Formation from Methyl Iodide, CO2 , and H2 by the Enzyme System Involved

1987 ◽  
Vol 42 (4) ◽  
pp. 360-372 ◽  
Author(s):  
Kerstin Laufer ◽  
Bernhard Eikmanns ◽  
Ursula Frimmer ◽  
Rudolf K. Thauer
Keyword(s):  
Methyl Iodide ◽  
Condensation Reaction ◽  
Methyl Chloride ◽  
Methanosarcina Barkeri ◽  
Carboxyl Group ◽  
Atpase Inhibitor ◽  
Methyl Group ◽  
Proton Potential ◽  
Acetate Formation ◽  
Reducing Equivalents

Cell suspensions of Methanosarcina barkeri grown on acetate catalyze the formation of methane and CO2 from acetate as well as an isotopic exchange between the carboxyl group of acetate and CO2. Here we report that these cells also mediate the synthesis of acetate from methyl iodide, CO2, and reducing equivalents (H2 or CO), the methyl group of acetate being derived from methyl iodide and the carboxyl group from CO2. Methyl chloride and methyltosylate but not methanol can substitute for methyl iodide in this reaction. Acetate formation from methyl iodide, CO2, and reducing equivalents is coupled with the phosphorylation of ADP. Evidence is pres­ented that methyl iodide is incorporated into the methyl group of acetate via a methyl corrinoid intermediate (deduced from inhibition experiments with propyl iodide) and that CO2 is assimi­lated into the carboxyl group via a C1 intermediate which does not exchange with free formate or free CO. The effects of protonophores, of the proton-translocating ATPase inhibitor N.N′-di- cyclohexylcarbodiimide, and of arsenate on acetate formation are interpreted to indicate that the reduction of CO2 to the oxidation level of the carboxyl group of acetate requires the presence of an electrochemical proton potential and that acetyl-CoA or acetyl-phosphate rather than free acetate is the immediate product of the condensation reaction. These results are discussed with respect to the mechanism of methanogenesis from acetate.

Sign in / Sign up

Export Citation Format

Share Document