Presence of luminal K+, a prerequisite for active NaCl transport in the cortical thick ascending limb of Henle's loop of rabbit kidney

1981 ◽  
Vol 392 (1) ◽  
pp. 92-94 ◽  
Author(s):  
R. Greger ◽  
E. Schlatter
Keyword(s):  
Rabbit Kidney ◽  
Thick Ascending Limb ◽  
Nacl Transport ◽  
Henle's Loop

AVP dynamically increases paracellular Na+ permeability and transcellular NaCl transport in the medullary thick ascending limb of Henle’s loop

2016 ◽  
Vol 469 (1) ◽  
pp. 149-158 ◽  
Author(s):  
Nina Himmerkus ◽  
Allein Plain ◽  
Rita D. Marques ◽  
Svenja R. Sonntag ◽  
Alexander Paliege ◽  
...  
Keyword(s):  
Thick Ascending Limb ◽  
Nacl Transport ◽  
Medullary Thick Ascending Limb ◽  
Henle's Loop ◽  
Na Permeability

Cyclooxygenase inhibitors increase Na-K-2Cl cotransporter abundance in thick ascending limb of Henle’s loop

1999 ◽  
Vol 277 (2) ◽  
pp. F219-F226 ◽  
Author(s):  
Patricia Fernández-Llama ◽  
Carolyn A. Ecelbarger ◽  
Joseph A. Ware ◽  
Peter Andrews ◽  
Alanna J. Lee ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Cyclooxygenase Inhibitors ◽  
Thick Ascending Limb ◽  
Outer Medulla ◽  
Nacl Transport ◽  
Expression Levels ◽  
Henle's Loop ◽  
Urinary Concentrating Ability ◽  
Ep3 Receptor ◽  
Type 3

Cyclooxygenase inhibitors, such as indomethacin and diclofenac, have well-described effects to enhance renal water reabsorption and urinary concentrating ability. Concentrating ability is regulated in part at the level of the thick ascending limb of Henle’s loop, where active NaCl absorption drives the countercurrent multiplication mechanism. We used semiquantitative immunoblotting to test the effects of indomethacin and diclofenac, given over a 48-h period, on the expression levels of the ion transporters responsible for active NaCl transport in the thick ascending limb. Both agents strongly increased the expression level of the apical Na-K-2Cl cotransporter in both outer medulla and cortex. Neither agent significantly altered outer medullary expression levels of other thick ascending limb proteins, namely, the type 3 Na/H exchanger (NHE-3), Tamm-Horsfall protein, or α1- or β1-subunits of the Na-K-ATPase. Administration of the EP3-selective PGE2analog, misoprostol, to indomethacin-treated rats reversed the stimulatory effect of indomethacin on Na-K-2Cl cotransporter expression. We conclude that cyclooxygenase inhibitors enhance urinary concentrating ability in part through effects to increase Na-K-2Cl cotransporter expression in the thick ascending limb of Henle’s loop. This action is most likely due to elimination of an EP3-receptor-mediated tonic inhibitory effect of PGE2 on cAMP production.

Characterization of Aldose Reductase from the Thick Ascending Limb of Henle’s Loop of Rabbit Kidney

Nephron ◽  
2001 ◽  
Vol 89 (1) ◽  
pp. 73-81 ◽  
Author(s):  
R. Willi Grunewald ◽  
Angela Eckstein ◽  
Claudius H. Reisse ◽  
Gerhard A. Müller
Keyword(s):  
Aldose Reductase ◽  
Rabbit Kidney ◽  
Thick Ascending Limb ◽  
Henle's Loop

Active NaCl transport in the cortical thick ascending limb of Henle's loop of the mouse does not require the presence of bicarbonate

1992 ◽  
Vol 420 (3-4) ◽  
pp. 290-296 ◽  
Author(s):  
A. Di Stefano ◽  
R. Greger ◽  
C. de Rouffignac ◽  
M. Wittner
Keyword(s):  
Thick Ascending Limb ◽  
Nacl Transport ◽  
Henle's Loop

A potassium channel in the apical membrane of rabbit thick ascending limb of Henle's loop

1990 ◽  
Vol 258 (2) ◽  
pp. F244-F253 ◽  
Author(s):  
W. H. Wang ◽  
S. White ◽  
J. Geibel ◽  
G. Giebisch
Keyword(s):  
Apical Membrane ◽  
Rabbit Kidney ◽  
K Channel ◽  
Thick Ascending Limb ◽  
Dependent Manner ◽  
Patch Clamp Technique ◽  
Bath Solution ◽  
Henle's Loop ◽  
Voltage Dependent ◽  
Inside Out

We used the patch-clamp technique to study the activity of single potassium channels in the apical membrane of isolated thick ascending limbs of Henle's loop (TAL) of rabbit kidneys. In cell-attached patches with NaCl Ringer or high-K+ solution in the bath and 140 mM K+ in the pipette, an inwardly rectifying K+ channel was observed with an inward slope conductance of 22.0 +/- 0.5 pS and outward slope conductance of 10.2 +/- 0.3 pS at 22 degrees C (n = 15). The channel was highly selective for K+, with a calculated permeability ratio for K(+)-to-Na+ of 20:1 (n = 4). The open probability (Po) of the channel was 0.89 +/- 0.03 (n = 15) and was not voltage dependent. In inside-out patches with 140 mM K+ in both the bath and the pipette solutions, both Po and conductance of the channel were similar to that in cell-attached patches. Addition of 0.1 mM Ba2+ to the pipette solution reduced Po of the channel in a voltage-dependent manner. Lowering the pH of the bath solution from 7.4 to 6.9 or increasing Ca2+ concentration from 0 to 0.5 mM in inside-out patches did not alter either Po or conductance of the channel. Addition of 2 mM ATP to the bath solution completely inhibited channel activity. This ATP-induced inhibition was fully reversible and was found to be dependent on the ratio of ATP to ADP, since adding 1 mM ADP to the bath solution relieved the ATP-induced blockade. The property of this small-conductance K+ channel make it a likely candidate for recycling of K+ across the apical membrane of TAL of the rabbit kidney. ATP and ADP are possible intracellular regulators of the channel's activity.

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