The membrane of catecholamine storage vesicles of adrenal medulla catecholamine fluxes and ATPase activity

1971 ◽  
Vol 270 (4) ◽  
pp. 392-406 ◽  
Author(s):  
G. Taujgner
Keyword(s):  
Atpase Activity ◽  
Adrenal Medulla ◽  
Storage Vesicles ◽  
Catecholamine Fluxes ◽  
Catecholamine Storage

scholarly journals The effect of salts on catecholamine fluxes and adenosine triphosphatase activity in storage vesicles from the adrenal medulla

1971 ◽  
Vol 123 (2) ◽  
pp. 219-225 ◽  
Author(s):  
G. Taugner
Keyword(s):  
Potassium Chloride ◽  
Adrenal Medulla ◽  
Sodium Nitrate ◽  
Adenosine Triphosphatase ◽  
Sodium Succinate ◽  
Adenosine Triphosphatase Activity ◽  
Storage Vesicles ◽  
Sucrose Medium ◽  
Catecholamine Fluxes ◽  
Net Release

1. Influx and efflux of catecholamine and adenosine triphosphatase activity in storage vesicles from the adrenal medulla were studied with dl-[14C]adrenaline in different media. 2. The lowest values for flux and adenosine triphosphatase activity were observed in sucrose media in which an ATP-dependent influx of catecholamine compensated for an efflux of the same magnitude. Efflux in the presence or absence of ATP was similar. 3. In media containing sodium succinate or glutarate adenosine triphosphatase activity was higher and the ATP-dependent influx of catecholamine was about twice that observed in iso-osmotic sucrose medium. In the presence of ATP influx and efflux of catecholamine were balanced; in its absence there was a net release of catecholamine, since efflux was more than twice the influx. Efflux in the presence or absence of ATP was similar. 4. In media containing sodium or potassium chloride and in the presence of ATP influx and adenosine triphosphatase activity were further enhanced, but in the absence of ATP there was no further increase in influx, since catecholamine was released with or without ATP at the same rate. Efflux was therefore twice as high in the presence of ATP as in its absence. 5. Sodium nitrate suppressed the ATP-dependent influx nearly completely, but caused a greatly enhanced efflux, which was twice as high in the presence of ATP as in its absence. 6. The extinction of vesicular suspensions remained unchanged in the presence of ATP under conditions where the catecholamine efflux was balanced by the influx. Under conditions where the efflux was not compensated by influx, the extinction of the suspensions decreased in the presence of ATP more than in its absence.

The membrane of the catecholamine storage vesicles of the adrenal medulla

Histochemie ◽  
1972 ◽  
Vol 33 (3) ◽  
pp. 255-272 ◽  
Author(s):  
B. Agostini ◽  
G. Taugner
Keyword(s):  
Adrenal Medulla ◽  
Storage Vesicles ◽  
Catecholamine Storage

Reversibility of ATP hydrolysis in catecholamine storage vesicles from bovine adrenal medulla

1980 ◽  
Vol 315 (2) ◽  
pp. 129-138 ◽  
Author(s):  
G. Taugner ◽  
I. Wunderlich ◽  
D. Junker
Keyword(s):  
Adrenal Medulla ◽  
Atp Hydrolysis ◽  
Bovine Adrenal Medulla ◽  
Storage Vesicles ◽  
Catecholamine Storage

scholarly journals The effects of univalent anions on catecholamine fluxes and adenosine triphosphatase activity in storage vesicles from the adrenal medulla

1972 ◽  
Vol 130 (4) ◽  
pp. 969-973 ◽  
Author(s):  
G. Taugner
Keyword(s):  
Adrenal Medulla ◽  
Adenosine Triphosphatase ◽  
Adenosine Triphosphatase Activity ◽  
Bovine Adrenal Medulla ◽  
Storage Vesicles ◽  
Dependent Sequence ◽  
Catecholamine Fluxes

1. Influx and efflux of catecholamine and adenosine triphosphatase activity were studied in storage vesicles of bovine adrenal medulla. 2. In the absence of ATP the influx of catecholamine was slow and was not influenced by various anions, whereas the efflux increased in the sequence of anions given by the lyotrophic series. 3. In the presence of ATP the efflux was enhanced compared with that in the absence of ATP; the anion-dependent sequence, however, in which the efflux increased was the same as in the absence of ATP. 4. The ATP-dependent catecholamine influx and the adenosine triphosphatase activity are correlated. The sequence in which anions affect adenosine triphosphatase activity and catecholamine influx, however, is completely different from the lyotrophic anion series. 5. No correlation was found between adenosine triphosphatase activity and the efflux of catecholamine.

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