A double protein A-gold-silver staining method for tissue antigens in light microscopy

1992 ◽  
Vol 24 (2) ◽  
pp. 61-66 ◽  
Author(s):  
O. Fujimori
Keyword(s):  
Light Microscopy ◽  
Silver Staining ◽  
Staining Method ◽  
Protein A ◽  
Gold Silver ◽  
Tissue Antigens ◽  
Silver Staining Method

An acidic silver staining method for synaptonemal complexes under light microscopy

1984 ◽  
Vol 133 (1) ◽  
pp. 99-102 ◽  
Author(s):  
José Fernandez-Piqueras ◽  
Carlos Sentis Castaño ◽  
E. Rojo Garcia ◽  
A. Rodriguez Campos
Keyword(s):  
Light Microscopy ◽  
Silver Staining ◽  
Staining Method ◽  
Synaptonemal Complexes ◽  
Silver Staining Method

Nucleolar organizer activity at meiosis in wheat–rye hybrid plants

1986 ◽  
Vol 28 (2) ◽  
pp. 227-234 ◽  
Author(s):  
N. Cuñado ◽  
M. C. Cermeño ◽  
J. Orellana
Keyword(s):  
Silver Staining ◽  
Staining Method ◽  
Nucleolar Organizer ◽  
The Other ◽  
Nucleolar Organizer Regions ◽  
Binucleate Cells ◽  
Hybrid Plants ◽  
Nucleolar Organizer Activity ◽  
Silver Staining Method ◽  
Organizer Activity

Nucleoli and nucleolar organizer regions (NORs) have been studied by a silver staining method in all meiotic stages of wheat–rye hybrid plants. The maximum number of nucleoli per cell scored at meiotic prophase and tapetum binucleate cells indicates that only the NORs of 1B, 6B, and 5D wheat chromosomes are active, whereas that of chromosome IR (SAT) of rye is inactive. However, at diakinesis, metaphase and anaphase meiotic stages only chromosomes 1B and 6B show Ag-NOR as was reported previously in somatic metaphase. The absence of Ag-NOR on chromosome 5D has been imputed to its low nucleolar organizer activity, not detectable by silver staining, because of the small number of rDNA clusters it carries. On the other hand, the meiotic behaviour of chromosomes 1B and 6B has been studied at metaphase I and anaphase I, using the Ag-NORs as cytological markers.Key words: nucleolar organizer, Ag-NOR, meiosis, wheat–rye hybrids.

scholarly journals Applications of immunocolloids in light microscopy. IV. Use of photochemical silver staining in a simple and efficient double-staining technique.

1985 ◽  
Vol 33 (12) ◽  
pp. 1247-1251 ◽  
Author(s):  
C Manigley ◽  
J Roth
Keyword(s):  
Light Microscopy ◽  
Silver Staining ◽  
Colloidal Gold ◽  
Protein A ◽  
Staining Technique ◽  
Gold Complex ◽  
Double Staining ◽  
Red Color ◽  
Gold Immunolocalization ◽  
Red Coloration

We report the development of a new light-microscopic double-staining technique using colloidal gold as sole marker. The contrasting color to the red of colloidal gold is achieved by the application of photochemical silver reaction. The silver reaction, which is principally performed at the end of the first staining sequence, converts the red color of a gold-labeled reagent into black. This contrasts clearly with the red coloration that results from the second incubation sequence without silver reaction. For antigen double staining, the same protein A-gold complex can be used to provide the black and the red color, thus rendering the technique very economical. Alternatively, combination of protein A-gold immunolocalization and lectin-gold staining is possible, as is combined lectin-gold staining.

Sign in / Sign up

Export Citation Format

Share Document