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2021 ◽  
Author(s):  
Mercè Cuadras ◽  
Jacques Planas ◽  
Ana Celma ◽  
Lucas Regis ◽  
Inés M. de Torres ◽  
...  

Abstract Background: Lymph node (LN) status is a key prognostic factor in the decision-making process of prostate cancer (PCa) management. Sectioning and haematoxylin and eosin (H&E) staining technique remain the gold standard for the evaluation of LN metastases despite some limitations, especially low sensitivity in detecting an accurate tumour burden within the LN, as well as a subjective and time-consuming result. One-step nucleic acid amplification (OSNA) quantifies mRNA copies of cytokeratin 19 (CK19) in a fast, objective, automated, and reproducible way, raising a general interest to explore its utility for lymphatic metastasis identification in different malignancies.Methods: To present the latest evidence related to the detection of LN metastases in several tumours by using OSNA compared with the conventional H&E method, a systematic review of articles published since March 2021 was conducted using PubMed, Cochrane Library, and Web of Science databases. References from primary papers and review articles were checked to obtain further potential studies. Our procedure for evaluating records identified during the literature search followed the Preferred Reporting Items for Systematic Reviews and Meta-analyses criteria.Results: Twenty five studies were included. LN from six different groups of tumours: breast, gastrointestinal, gynecological, lung, head and neck and prostate cancers has been assessed. OSNA was compared with post-operative formalin-fixed paraffin-embedded tissue sections with H&E staining as the reference standard. Contingency tables were created, and concordance rate, sensitivity, specificity and predictive values were reported. Seventeen studies analysed the discordant cases using different techniques.Conclusion: OSNA method has a high diagnostic accuracy for the detection of LN metastases in several CK19 expressing tumours. Available evidence encourages its usage in PCa patients to improve LN staging and prognosis.


2021 ◽  
Vol 202 ◽  
pp. 104498
Author(s):  
Juan Felipe Ladino ◽  
Santiago Saavedra ◽  
Daniel Wiesner
Keyword(s):  
The Law ◽  

2021 ◽  
Author(s):  
Zhishan Wang ◽  
Xianyu Wu ◽  
Ni Li ◽  
Weiping Wang ◽  
Yang Liu

Abstract Screening for microorganisms with antagonistic activity against pathogens in crops is of great significance for the preparation of microbial antagonists, the control of crop diseases, and the sustainable development of agriculture. Through the experiment, a rapid, efficient, and one-step high-throughput method for screening endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice seeds was successfully established, and the experimental results showed that the endophytic bacteria with antagonistic activity of Magnaporthe oryzae ACCC 36020 could be directly screened from six groups of experimental samples by this method. The establishment of this method can achieve simultaneous screening and purification, one-step high-throughput screening of antagonistic bacteria in rice seeds, and greatly save time.


PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256813
Author(s):  
Maria Jose Lista ◽  
Pedro M. Matos ◽  
Thomas J. A. Maguire ◽  
Kate Poulton ◽  
Elena Ortiz-Zapater ◽  
...  

There is a worldwide need for reagents to perform SARS-CoV-2 detection. Some laboratories have implemented kit-free protocols, but many others do not have the capacity to develop these and/or perform manual processing. We provide multiple workflows for SARS-CoV-2 nucleic acid detection in clinical samples by comparing several commercially available RNA extraction methods: QIAamp Viral RNA Mini Kit (QIAgen), RNAdvance Blood/Viral (Beckman) and Mag-Bind Viral DNA/RNA 96 Kit (Omega Bio-tek). We also compared One-step RT-qPCR reagents: TaqMan Fast Virus 1-Step Master Mix (FastVirus, ThermoFisher Scientific), qPCRBIO Probe 1-Step Go Lo-ROX (PCR Biosystems) and Luna® Universal Probe One-Step RT-qPCR Kit (Luna, NEB). We used primer-probes that detect viral N (EUA CDC) and RdRP. RNA extraction methods provided similar results, with Beckman performing better with our primer-probe combinations. Luna proved most sensitive although overall the three reagents did not show significant differences. N detection was more reliable than that of RdRP, particularly in samples with low viral titres. Importantly, we demonstrated that heat treatment of nasopharyngeal swabs at 70°C for 10 or 30 min, or 90°C for 10 or 30 min (both original variant and B 1.1.7) inactivated SARS-CoV-2 employing plaque assays, and had minimal impact on the sensitivity of the qPCR in clinical samples. These findings make SARS-CoV-2 testing portable in settings that do not have CL-3 facilities. In summary, we provide several testing pipelines that can be easily implemented in other laboratories and have made all our protocols and SOPs freely available at https://osf.io/uebvj/.


2021 ◽  
Vol 172 ◽  
pp. 113962
Author(s):  
Xiuhong Zhong ◽  
Ran Yuan ◽  
Baoshuai Zhang ◽  
Bin Wang ◽  
Yaqi Chu ◽  
...  

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