Repairable and unrepairable DNA strand breaks induced by decay of3H and125I incorporated into DNA of mammalian cells

1980 ◽  
Vol 18 (4) ◽  
pp. 239-248 ◽  
Author(s):  
Synn�ve Sundell-Bergman ◽  
Karl J. Johanson
Keyword(s):  
Mammalian Cells ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
Dna Strand

Topoisomerase II inhibition prevents anaphase chromatid segregation in mammalian cells independently of the generation of DNA strand breaks

1993 ◽  
Vol 105 (2) ◽  
pp. 563-569 ◽  
Author(s):  
D.J. Clarke ◽  
R.T. Johnson ◽  
C.S. Downes
Keyword(s):  
Chromosome Segregation ◽  
Mammalian Cells ◽  
Topoisomerase Ii ◽  
Dna Strand Breaks ◽  
Temperature Sensitive ◽  
Dna Topoisomerase ◽  
Strand Breaks ◽  
Temperature Sensitive Mutants ◽  
Chromatid Segregation ◽  
Dna Strand

Yeast temperature-sensitive mutants of DNA topoisomerase II are incapable of chromosome condensation and anaphase chromatid segregation. In mammalian cells, topoisomerase II inhibitors such as etoposide (VP-16-123) have similar effects. Unfortunately, conclusions drawn from work with mammalian cells have been limited by the fact that the standard inhibitors of topoisomerase II also generate DNA strand breaks, which when produced by other agents (e.g. ionizing radiation) are known to affect progression into and through mitosis. Here we show that the anti-tumour agent ICRF-193, recently identified as a topoisomerase II inhibitor operating by a non-standard mechanism, generates neither covalent complexes between topoisomerase II and DNA, nor adjacent DNA strand breaks, in mitotic HeLa. However, the drug does prevent anaphase segregation in HeLa and PtK2 cells, with effects similar to those of etoposide. We therefore conclude that topoisomerase II function is required for anaphase chromosome segregation in mammalian cells, as it is in yeast.

Method for detecting DNA strand breaks in mammalian cells using the Deinococcus radiodurans PprA protein

2006 ◽  
Vol 596 (1-2) ◽  
pp. 36-42 ◽  
Author(s):  
Katsuya Satoh ◽  
Seiichi Wada ◽  
Masahiro Kikuchi ◽  
Tomoo Funayama ◽  
Issay Narumi ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Deinococcus Radiodurans ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
Dna Strand

Rejoining of DNA strand breaks by T4 DNA ligase in mammalian cells

2002 ◽  
Vol 41 (2) ◽  
pp. 149-153 ◽  
Author(s):  
T. Ortiz ◽  
A. Edreira ◽  
J. Piñero
Keyword(s):  
Mammalian Cells ◽  
Dna Strand Breaks ◽  
Dna Ligase ◽  
T4 Dna Ligase ◽  
Strand Breaks ◽  
Dna Strand

Fluorometric Analysis of DNA Unwinding (FADU) as a Method for Detecting Repair-induced DNA Strand Breaks in UV-irradiated Mammalian Cells¶

2000 ◽  
Vol 72 (4) ◽  
pp. 477 ◽  
Author(s):  
Christa Baumstark-Khan ◽  
Uwe Hentschel ◽  
Yelizabeta Nikandrova ◽  
Jens Krug ◽  
Gerda Horneck
Keyword(s):  
Mammalian Cells ◽  
Dna Strand Breaks ◽  
Dna Unwinding ◽  
Strand Breaks ◽  
Fluorometric Analysis ◽  
Dna Strand

Detection of lignin biopolymer‐ and vitamin e‐stimulated reduction of DNA strand breaks in H2O2‐ and MNNG‐treated mammalian cells by the comet assay

1999 ◽  
Vol 33 (1) ◽  
pp. 88-94 ◽  
Author(s):  
Darina Slamenová ◽  
Eva Horváthová ◽  
Božena Košíková ◽  
L'ubica Ružeková ◽  
Juraj Lábaj
Keyword(s):  
Vitamin E ◽  
Comet Assay ◽  
Mammalian Cells ◽  
Dna Strand Breaks ◽  
Strand Breaks ◽  
Dna Strand
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