The amino acid sequence and reactive site of a single-headed trypsin inhibitor from wheat endosperm

1994 ◽  
Vol 13 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Elia Poerio ◽  
Carlo Caporale ◽  
Lucia Carrano ◽  
Carla Caruso ◽  
Francesco Vacca ◽  
...  
1996 ◽  
Vol 43 (3) ◽  
pp. 489-496 ◽  
Author(s):  
A Wilimowska-Pelc ◽  
D Stachowiak ◽  
M Gładysz ◽  
Z Olichwier ◽  
A Polanowski

A trypsin inhibitor of Kazal type has been isolated from goose pancreas by affinity chromatography on immobilized anhydrotrypsin, anion exchange and reverse phase HPLC. It inhibits bovine beta-trypsin with the association constant (Ka) of 5.99 x 10(8) M-1. The complete amino-acid sequence was determined following CNBr treatment. The protein comprised a total of 69 amino-acid residues, corresponding to a molecular mass of 7.7 kDa. The P1-P'1 reactive site bond of the inhibitor was localized at position Lys25-Met26. The amino-acid sequence of GPTI shows extremely high homology to that of other inhibitors isolated from pancreas of birds.


1993 ◽  
Vol 103 (4) ◽  
pp. 1407-1412 ◽  
Author(s):  
S. Valdes-Rodriguez ◽  
M. Segura-Nieto ◽  
A. Chagolla-Lopez ◽  
AVy. Vargas-Cortina ◽  
N. Martinez-Gallardo ◽  
...  

FEBS Letters ◽  
1985 ◽  
Vol 191 (2) ◽  
pp. 269-272 ◽  
Author(s):  
Norihisa Kikuchi ◽  
Kiyoshi Nagata ◽  
Nobuo Yoshida ◽  
Tatsuhiko Tanaka ◽  
Masahiro Yamamoto ◽  
...  

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