Synaptic vesicle depletion and glutamate uptake in a nerve-muscle preparation of the locust,Locusta migratoria L

1978 ◽  
Vol 34 (2) ◽  
pp. 209-210 ◽  
Author(s):  
R. P. Botham ◽  
D. J. Beadle ◽  
R. J. Hart ◽  
C. Potter ◽  
R. G. Wilson
Keyword(s):  
Synaptic Vesicle ◽  
Glutamate Uptake ◽  
Locusta Migratoria ◽  
Muscle Preparation ◽  
Nerve Muscle

scholarly journals GLUTAMATE UPTAKE BY A STIMULATED INSECT NERVE MUSCLE PREPARATION

1970 ◽  
Vol 46 (2) ◽  
pp. 300-307 ◽  
Author(s):  
Isabelle R. Faeder ◽  
Miriam M. Salpeter
Keyword(s):  
Electron Microscope ◽  
Nerve Stimulation ◽  
Neuromuscular Junctions ◽  
Tissue Concentration ◽  
Glutamate Uptake ◽  
Muscle Preparation ◽  
Glutamate Concentration ◽  
Chemical Studies ◽  
Nerve Muscle ◽  
Cellular Compartments

Recent reports suggest that glutamate may be the excitatory neuromuscular transmitter in insects. In this study, glutamate uptake by isolated cockroach nerve muscle preparations was investigated by means of chemical and electron microscope radioautographic techniques. We found that the preparation had a high affinity for glutamate and that nerve stimulation enhanced glutamate uptake. Chemical studies showed that the average tissue concentration of glutamate bound during a 1 hr incubation period in 10-5 M glutamate-3H after nerve stimulation was 2.8 x 10-5 M. Less than 1% of the radioactivity was present in the perchloric acid-precipitated protein fraction. Using electron microscope radioautography, we observed that sheath cells showed the highest glutamate concentration of all cellular compartments. Uptake was greater at neuromuscular junctions than in other regions of the tissue. The data suggest a possible mechanism for transmitter inactivation and protection of synapses from high blood glutamate.

Effect of armin on synaptic transmission in the frog nerve-muscle preparation

1981 ◽  
Vol 91 (6) ◽  
pp. 774-777 ◽  
Author(s):  
I. I. Krivoi ◽  
V. I. Kuleshov ◽  
D. P. Matyushkin ◽  
V. I. Sanotskii ◽  
I. A. Shabunova
Keyword(s):  
Synaptic Transmission ◽  
Muscle Preparation ◽  
Nerve Muscle

A Mammalian Nerve—Muscle Preparation Suitable for Single-Fiber Experiments

Science ◽  
1951 ◽  
Vol 113 (2933) ◽  
pp. 300-300
Author(s):  
Vernon B. Brooks
Keyword(s):  
Single Fiber ◽  
Muscle Preparation ◽  
Nerve Muscle ◽  
Mammalian Nerve

Effect of Bothrops insularis venom on the mouse and chick nerve-muscle preparation

Toxicon ◽  
1993 ◽  
Vol 31 (10) ◽  
pp. 1237-1247 ◽  
Author(s):  
J.C. Cogo ◽  
J. Prado-Franceschi ◽  
M.A. Cruz-Hofling ◽  
A.P. Corrado ◽  
L. Rodrigues-Simioni
Keyword(s):  
Muscle Preparation ◽  
Nerve Muscle

scholarly journals I. Croonian Lecture .—Observations on isolated nerve (with particular reference to carbon dioxide)

1897 ◽  
Vol 188 ◽  
pp. 1-101 ◽  
Keyword(s):  
Weak Link ◽  
Nerve Degeneration ◽  
Muscle Preparation ◽  
Marked Contrast ◽  
Functional Failure ◽  
Du Bois ◽  
Motor End Plates ◽  
Nerve Muscle ◽  
Long Endurance ◽  
Experimental Disturbance

As was pointed out ten years ago by Wedenski, from telephone observations of the negative variation, by Waller, from galvanometric observations of the same, and by Bowditch from the observation of long-persisting excitability in the nerves of temporarily curarised mammals, nerve is now admitted to be practically inexhaustible. The apparent exhaustion of nerve when a nerve-muscle preparation is submitted to prolonged excitation is, as was first demonstrated in Bernstein’s experiment, not an exhaustion of nerve, but an exhaustion of the organ of intermediation between nerve and muscle, which, as pointed out by Waller, is the weak link in the neuro-muscular chain, being the point at which functional failure is first manifested, in fatigue, in nerve degeneration, in intoxication by curare (Bernard), and at death. Taking as the sign of action artificially excited in a nerve, the negative variation of its current of rest (du Bois-Reymond) or current of injury (Hermann), the long endurance by nerve of excitation excessive in strength and in length, is well shown by prolonged photographic records of the galvanometric indications, which exhibit little or no decline, in marked contrast with similar records of the negative variation of muscle which declines pari passu with the declining contraction due to fatigue of the motor end plates. This steady regularity of response renders nerve pre-eminently suitable to the systematic study of its experimental disturbance.

scholarly journals Org NC 45 Reduces Quantal Release of Acetylcholine (a Preliminary Communication)

1982 ◽  
Vol 10 (2) ◽  
pp. 127-129 ◽  
Author(s):  
T. A. Torda ◽  
N. Kiloh
Keyword(s):  
Neuromuscular Blocker ◽  
Variance Ratio ◽  
Muscle Preparation ◽  
Quantal Release ◽  
End Plate ◽  
Release Of Acetylcholine ◽  
Preliminary Communication ◽  
Nerve Muscle

The new neuromuscular blocker, Org NC 45 was applied to the toad sartorius nerve-muscle preparation. Measurement of the variance ratio of end-plate potentials recorded by micro-electrodes demonstrated that in concentrations greater than that required to block myoneural transmission, Org NC 45 reduced the quantal release of acetylcholine.

Varespladib (LY315920) inhibits neuromuscular blockade induced by Oxyuranus scutellatus venom in a nerve-muscle preparation

Toxicon ◽  
2020 ◽  
Vol 187 ◽  
pp. 101-104
Author(s):  
Isadora Caruso Fontana Oliveira ◽  
José María Gutiérrez ◽  
Matthew R. Lewin ◽  
Yoko Oshima-Franco
Keyword(s):  
Neuromuscular Blockade ◽  
Muscle Preparation ◽  
Nerve Muscle
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