Prostaglandins have no effect on spontaneous and gonadotrophin-releasing hormone stimulated luteinizing hormone release in cultured rat pituitary cells

1984 ◽  
Vol 234 (4) ◽  
pp. 263-268 ◽  
Author(s):  
Günter Emons ◽  
Sabine Thiessen ◽  
Ulrich Gethmann
Keyword(s):  
Luteinizing Hormone ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Releasing Hormone ◽  
Luteinizing Hormone Release ◽  
Rat Pituitary ◽  
Gonadotrophin Releasing Hormone ◽  
Rat Pituitary Cells

Stimulation of luteinizing hormone release by phospholipases and melittin in rat pituitary cells

1985 ◽  
Vol 110 (1_Suppla) ◽  
pp. S9-S10 ◽  
Author(s):  
L. KIESEL ◽  
T. RABE ◽  
G. HAUSER ◽  
B. RUNNEBAUM
Keyword(s):  
Luteinizing Hormone ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Luteinizing Hormone Release ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Stimulation Of

Effects of Inhibin from Primate Sertoli Cells on Follicle-Stimulating Hormone and Luteinizing Hormone Release by Perifused Rat Pituitary Cells*

Endocrinology ◽  
1988 ◽  
Vol 122 (6) ◽  
pp. 2796-2802 ◽  
Author(s):  
FUMIKAZU KOTSUJI ◽  
STEPHEN J. WINTERS ◽  
HUGH S. KEEPING ◽  
BARBARA ATTARDI ◽  
HIROYUKI OSHIMA ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Sertoli Cells ◽  
Follicle Stimulating Hormone ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Luteinizing Hormone Release ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Stimulating Hormone

Pituitary gonadotrophin-releasing hormone receptor up-regulation in vitro: dependence on calcium and microtubule function

1985 ◽  
Vol 107 (1) ◽  
pp. 49-56 ◽  
Author(s):  
L. S. Young ◽  
S. I. Naik ◽  
R. N. Clayton
Keyword(s):  
Luteinizing Hormone ◽  
Gnrh Receptor ◽  
Ionophore A23187 ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Releasing Hormone ◽  
Luteinizing Hormone Release ◽  
Gnrh Receptors ◽  
Gonadotrophin Releasing Hormone

ABSTRACT Exogenous cyclic adenosine nucleotides increase gonadotrophin-releasing hormone (GnRH) receptors in intact cultured rat pituitary cells in a similar manner to that observed with GnRH itself. In this study the calcium and microtubule dependency of GnRH receptor up-regulation was examined in vitro. Treatment of pituitary cells in Ca2+ and serum-containing media with either GnRH (1 nmol/l), K+ (58 mmol/l) or dibutyryl cyclic AMP (dbcAMP; 1 mmol/l) for 7–10 h routinely resulted in a 50–100% increase in GnRH receptors. Incubation of pituitary cells with the calcium channel blocker verapamil, for 7 h, or the calcium chelator EGTA, for 10 h, had no effect on basal receptor levels but prevented the increase in GnRH receptors stimulated by either GnRH, K+ or dbcAMP. Luteinizing hormone release measured with the same stimulators over a 3-h period was prevented by both verapamil and EGTA. Calcium ionophore (A23187) increased GnRH receptors by 40–60% at low concentrations (10 and 100 nmol/l) while higher concentrations (10 and 100 μmol/l) reduced receptor levels. Luteinizing hormone release was not increased by receptor-stimulating concentrations of A23187, but was by higher concentrations (10 μmol/l). None of these pretreatments, for up to 10 h, impaired the subsequent LH response of the cells to increasing doses of GnRH. Vinblastine (1 μmol/l did not affect basal receptor levels but markedly reduced the increase in GnRH receptors stimulated by GnRH, K+ and dbcAMP. This concentration of vinblastine had no effect on LH release. These results indicate that receptor stimulation by GnRH, K+ and dbcAMP is a calcium-dependent process requiring the integrity of the microtubule system and there is a different calcium requirement for the processes of GnRH receptor up-regulation and LH secretion. J. Endocr. (1985) 107, 49–56

Stimulation of luteinizing hormone release by melittin and phospholipase A2 in rat pituitary cells

1987 ◽  
Vol 51 (1-2) ◽  
pp. 1-6 ◽  
Author(s):  
Ludwig Kiesel ◽  
Thomas Rabe ◽  
Gabi Hauser ◽  
Andrzej Przylipiak ◽  
Fayegh Jadali ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Phospholipase A2 ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Luteinizing Hormone Release ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Stimulation Of

Exogenous action of 5-lipoxygenase by its metabolites on luteinizing hormone release in rat pituitary cells

1990 ◽  
Vol 69 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Andrzej Przylipiak ◽  
Ludwig Kiesel ◽  
Andreas J.R. Habenicht ◽  
Maria Przylipiak ◽  
Benno Runnebaum
Keyword(s):  
Luteinizing Hormone ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Luteinizing Hormone Release ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

Melatonin inhibits luteinizing hormone releasing hormone (LHRH) induction of LH release from fetal rat pituitary cells

1995 ◽  
Vol 184 (2) ◽  
pp. 109-112 ◽  
Author(s):  
Atsuhiko Hattori ◽  
Damon C. Herbert ◽  
Mary K. Vaughan ◽  
Ken Yaga ◽  
Russel J. Reiter
Keyword(s):  
Luteinizing Hormone ◽  
Pituitary Cells ◽  
Luteinizing Hormone Releasing Hormone ◽  
Releasing Hormone ◽  
Fetal Rat ◽  
Rat Pituitary ◽  
Lh Release ◽  
Rat Pituitary Cells
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