Stimulation of bile acid synthesis by dibutyryl cyclic AMP in isolated rat hepatocytes

Lipids ◽  
1983 ◽  
Vol 18 (7) ◽  
pp. 443-447 ◽  
Author(s):  
G. S. Sundaram ◽  
Vicki Rothman ◽  
Simeon Margolis
Keyword(s):  
Bile Acid ◽  
Cyclic Amp ◽  
Rat Hepatocytes ◽  
Acid Synthesis ◽  
Bile Acid Synthesis ◽  
Dibutyryl Cyclic Amp ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat ◽  
Stimulation Of

Effect of oestradiol and progesterone on bile acid synthesis in isolated rat hepatocytes

1989 ◽  
Vol 17 (5) ◽  
pp. 884-885 ◽  
Author(s):  
MARIA A. ALONSO ◽  
KATHLEEN M. BOTHAM ◽  
BEGOÑA OCHOA
Keyword(s):  
Bile Acid ◽  
Rat Hepatocytes ◽  
Acid Synthesis ◽  
Bile Acid Synthesis ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

scholarly journals Stimulatory effect of the intestinal peptide PHI on glycogenolysis and gluconeogenesis in isolated rat hepatocytes

1983 ◽  
Vol 214 (3) ◽  
pp. 999-1002 ◽  
Author(s):  
J E Felíu ◽  
J Marco
Keyword(s):  
Cyclic Amp ◽  
Pyruvate Kinase ◽  
Glycogen Phosphorylase ◽  
Rat Hepatocytes ◽  
Fold Increase ◽  
Isolated Rat Hepatocytes ◽  
Mediated Effects ◽  
Dose Dependent ◽  
Isolated Rat ◽  
Stimulation Of

The newly isolated peptide PHI provoked a dose-dependent stimulation of glycogenolysis and gluconeogenesis in isolated rat hepatocytes; at 1 microM-PHI, both processes were increased 1.6-fold as compared with basal values. These PHI-mediated effects were accompanied by the activation of glycogen phosphorylase and the inactivation of pyruvate kinase. PHI (1 microM) also caused a 2-fold increase in hepatocyte cyclic AMP.

scholarly journals Different modulatory effects of elevated cyclic AMP on cytosolic Ca2+ spikes induced by phenylephrine or vasopressin in single rat hepatocytes

1993 ◽  
Vol 291 (1) ◽  
pp. 163-168 ◽  
Author(s):  
A Sanchez-Bueno ◽  
I Marrero ◽  
P H Cobbold
Keyword(s):  
Cyclic Amp ◽  
Rat Hepatocytes ◽  
Specific Information ◽  
Dibutyryl Cyclic Amp ◽  
Isolated Rat Hepatocytes ◽  
Frequency Peak ◽  
Isolated Rat

We show here, by aequorin measurements in single isolated rat hepatocytes, that elevation of cyclic AMP, by dibutyryl cyclic AMP, forskolin or glucagon, has different effects on oscillations in cytosolic concentration of free Ca2+ (‘free Ca’) induced by phenylephrine or vasopressin. Elevated cyclic AMP does not itself induce free Ca oscillations, but enhances both the peak free Ca and the frequency of spikes induced by phenylephrine. In contrast, elevated cyclic AMP has no effect on peak free Ca of vasopressin-induced spikes, but markedly prolongs the falling phase, with the result that spiking frequency (peak to peak) falls, although the period between spikes of resting free Ca is usually decreased. The data provide another example of receptor-specific information being retained in the oscillator mechanism, with implications for models of the hepatocyte calcium oscillator.

Bile acid synthesis in isolated rat hepatocytes

1978 ◽  
Vol 56 (8) ◽  
pp. 780-783 ◽  
Author(s):  
I. M. Yousef ◽  
J. Ho ◽  
K. N. Jeejeebhoy
Keyword(s):  
Bile Acid ◽  
Bile Acids ◽  
Rat Hepatocytes ◽  
Acid Synthesis ◽  
Isolated Hepatocytes ◽  
Bile Acid Synthesis ◽  
Isolated Rat Hepatocytes ◽  
Adult Rat ◽  
Vitro Model

Normal adult rat hepatocytes were incubated for 48 h and the concentration of total and individual bile acids in homogenized samples of the culture was measured at intervals during the incubation, using radiogas chromatography and isotope derivative assay. The net increase in bile acids over the value observed at the start of the culture was taken as synthesis. The results showed that bile acid synthesis was linear up to 24 h of incubation, at a rate of 20 nmol/g hepatocytes per hour, and that 85% of the newly synthesized bile acid was cholic acid. The bile acid synthesized was mainly conjugated with taurine. These results suggest that isolated hepatocytes cultured in the way described could be a useful in vitro model for the study of bile acid synthesis.

scholarly journals The effects of orthovanadate on fatty acid synthesis in isolated rat hepatocytes

1982 ◽  
Vol 202 (3) ◽  
pp. 791-794 ◽  
Author(s):  
L Agius ◽  
W J Vaartjes
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Rat Hepatocytes ◽  
Acid Synthesis ◽  
Insulin Stimulation ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat ◽  
Stimulation Of

Extracellular Ca2+ stimulated fatty acid synthesis in isolated rat hepatocytes. Orthovanadate (0.2-2.0 mM), an inhibitor of Ca2+-dependent ATPases, stimulated fatty acid synthesis in both the presence and the absence of extracellular Ca2+. Insulin stimulated fatty acid synthesis only in the presence of extracellular Ca2+. The contribution of extracellular Ca2+ to insulin stimulation of fatty acid synthesis is discussed.

scholarly journals Effects of phorbol esters on α1-adrenergic-mediated and glucagon-mediated actions in isolated rat hepatocytes

1985 ◽  
Vol 228 (1) ◽  
pp. 277-280 ◽  
Author(s):  
J A García-Sáinz ◽  
F Mendlovic ◽  
M A Martínez-Olmedo
Keyword(s):  
Cyclic Amp ◽  
Dose Response ◽  
Response Curve ◽  
Phorbol Esters ◽  
Rat Hepatocytes ◽  
Metabolic Effects ◽  
Isolated Rat Hepatocytes ◽  
The Right ◽  
Isolated Rat ◽  
Stimulation Of

Phorbol 12-myristate 13-acetate (PMA) inhibited the stimulation of ureogenesis produced by adrenaline, but produced a minimal displacement to the right of the dose-response curve for glucagon. However, PMA diminished the accumulation of cyclic AMP induced by glucagon. Dissociation between the cyclic AMP concentrations and the metabolic effects induced by glucagon is evidenced in the presence of phorbol esters.

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