Characterization and culture of virus replicating continuous insect cell lines from the bollworm,Heliothis zea (boddie)

In Vitro ◽  
1982 ◽  
Vol 18 (10) ◽  
pp. 843-850 ◽  
Author(s):  
Ronald H. Goodwin ◽  
George J. Topkins ◽  
Russell R. Gettig ◽  
Jean R. Adams
Author(s):  
J. R. Adams ◽  
R. H. Goodwin ◽  
T. A. Wilcox

Some insect tissue culture cells may be readily infected with inocula of infectious hemolymph from the respective diseased host species or infectious cell culture supernatants from the respective infected tissue culture cells. We were interested in studying the degree of specificity of several insect cell lines to non-host viruses. Viruses investigated were baculoviruses (BV) isolated from corn earworm, Heliothis zea (Boddie); cabbage looper, Trichoplusia ni (Hübner) ; fall armyworm, Spodoptera frugiperda (J. D. Smith); alfalfa looper, Autographa californica (Speyer); gypsy moth, Porthetria dispar (L.) and cotton bollworm, Heliothis armigera (Hubner) on cell lines obtained from T. ni, H. zea, S. frugiperda.


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