Studies on the Specificity of Several Insect Cell Lines to Replication of Baculoviruses

1974 ◽  
Vol 32 ◽  
pp. 260-261
Author(s):  
J. R. Adams ◽  
R. H. Goodwin ◽  
T. A. Wilcox
Keyword(s):  
Tissue Culture ◽  
Cell Lines ◽  
Insect Cell ◽  
Trichoplusia Ni ◽  
Heliothis Zea ◽  
Corn Earworm ◽  
Cabbage Looper ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Insect Cell Lines

Some insect tissue culture cells may be readily infected with inocula of infectious hemolymph from the respective diseased host species or infectious cell culture supernatants from the respective infected tissue culture cells. We were interested in studying the degree of specificity of several insect cell lines to non-host viruses. Viruses investigated were baculoviruses (BV) isolated from corn earworm, Heliothis zea (Boddie); cabbage looper, Trichoplusia ni (Hübner) ; fall armyworm, Spodoptera frugiperda (J. D. Smith); alfalfa looper, Autographa californica (Speyer); gypsy moth, Porthetria dispar (L.) and cotton bollworm, Heliothis armigera (Hubner) on cell lines obtained from T. ni, H. zea, S. frugiperda.

Characterization and culture of virus replicating continuous insect cell lines from the bollworm,Heliothis zea (boddie)

In Vitro ◽  
1982 ◽  
Vol 18 (10) ◽  
pp. 843-850 ◽  
Author(s):  
Ronald H. Goodwin ◽  
George J. Topkins ◽  
Russell R. Gettig ◽  
Jean R. Adams
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Heliothis Zea ◽  
Insect Cell Lines

scholarly journals Mucin-type proteins produced in the Trichoplusia ni and Spodoptera frugiperda insect cell lines carry novel O-glycans with phosphocholine and sulfate substitutions

Glycobiology ◽  
2013 ◽  
Vol 23 (7) ◽  
pp. 778-796 ◽  
Author(s):  
Stefan Gaunitz ◽  
Chunsheng Jin ◽  
Anki Nilsson ◽  
Jining Liu ◽  
Niclas G Karlsson ◽  
...  
Keyword(s):  
Cell Lines ◽  
Spodoptera Frugiperda ◽  
Insect Cell ◽  
Trichoplusia Ni ◽  
Insect Cell Lines

scholarly journals A recombinant baculovirus TnGV in Trichoplusia ni larvae using the PIG bombardment method and the CRISPR/Cas9 system

2021 ◽  
Author(s):  
Oscar J. Ortiz-Arrazola ◽  
Maria Cristina Del Rincon-Castro
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Trichoplusia Ni ◽  
High Efficiency ◽  
Recombinant Baculovirus ◽  
Heterologous Proteins ◽  
Reporter Protein ◽  
Insect Cell Lines ◽  
Specific Sequences

Baculoviruses have been used for the expression of heterologous proteins of biotechnological interest. However, most of these proteins are obtained by homologous co-transfection recombination in cell lines, limiting their use. Recently, the CRISPR/Cas9 system has excelled in its high efficiency in editing specific sequences without the need for insect cell lines. In this work, the CRISPR/Cas9 system was used to edit the genome of Trichopusia ni granulovirus (TnGV) and transformation of insects by the PIG bombardment method. A homologous repair vector (pTnGV101) was designed with regions orf5 and orf7, as well as sgRNA flanking TnGV P10 of this virus. The bombardment transformation was carried out at 175 psi with 40% of infected T. ni larvae, of which 38% expressed the reporter protein EGFP. These results demonstrate that the CRISPR/Cas9 system and PIG bombardment can be used for genetic modification of baculovirus in vivo.

scholarly journals A study of communication specificity between cells in culture.

1977 ◽  
Vol 75 (3) ◽  
pp. 769-787 ◽  
Author(s):  
M L Epstein ◽  
N B Gilula
Keyword(s):  
Gap Junctions ◽  
Cell Lines ◽  
Insect Cell ◽  
Cell Types ◽  
Cells In Culture ◽  
Culture Cells ◽  
Low Incidence ◽  
Insect Cell Lines ◽  
Gap Junctional ◽  
Ionic Coupling

We have examined the specificity of communication between cells in culture by co-culturing cells derived from mammalian, avian, and arthropod organisms. Both mammalian and avian culture cells have similar gap junctional phenotypes, while the insect (arthropod) cell lines have a significantly different gap junctional structure. Electrophysiological and ultrastructural methods were used to examine ionic coupling and junctional interactions between homologous and heterologous cell types. In homologous cell systems, gap junctions and ionic coupling are present at a high incidence. Also, heterologous vertebrate cells in co-culture can communicate readily. By contrast, practically no coupling (0-8%) is detectable between heterologous insect cell lines (Homopteran or Lepidopteran) and vertebrate cells (mammalian myocardial or 3T3 cells). No gap junctions have been observed between arthropod and vertebrate cell types, even though the heterologous cells may be separated by less than 10 nm. In additional studies, a low incidence of coupling was found between heterologous insect cell lines derived from different arthropod orders. However, extensive coupling was detected between insect cell lines that are derived from the same order (Homoptera). These observations suggest that there is little or no apparent specificity for communication between vertebrate cells in culture that express the same gap junctional phenotype, while there is a definite communication specificity that exists between arthropod cells in culture.

Cytoplasmic Tubules in Cells Infected with Laryngotracheitis Virus

1969 ◽  
Vol 27 ◽  
pp. 376-377
Author(s):  
A. M. Watrach
Keyword(s):  
Tissue Culture ◽  
Small Proportion ◽  
Chicken Embryo ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Morphologic Characteristics ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
In Cells

During a study of the development of infectious laryngotracheitis (LT) virus in tissue culture cells, unusual tubular formations were found in the cytoplasm of a small proportion of the affected cells. It is the purpose of this report to describe the morphologic characteristics of the tubules and to discuss their possible association with the development of virus.The source and maintenance of the strain of LT virus have been described. Prior to this study, the virus was passed several times in chicken embryo kidney (CEK) tissue culture cells.

Sulfhydryl bond formation is a prerequisite for proper cycling of centrosomes and chromosomes in mammalian tissue culture cells

1993 ◽  
Vol 51 ◽  
pp. 342-343
Author(s):  
Heide Schatten ◽  
Neidhard Paweletz ◽  
Ron Balczon
Keyword(s):  
Tissue Culture ◽  
Cell Cycle Progression ◽  
Group Formation ◽  
Sulfhydryl Group ◽  
Mammalian Tissue ◽  
Mitotic Chromosomes ◽  
Microtubule Network ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Transmission Electron

To study the role of sulfhydryl group formation during cell cycle progression, mammalian tissue culture cells (PTK2) were exposed to 100¼M 2-mercaptoethanol for 2 to 6 h during their exponential phase of growth. The effects of 2-mercaptoethanol on centrosomes, chromosomes, microtubules, membranes and intermediate filaments were analyzed by transmission electron microscopy (TEM) and by immunofluorescence microscopy (IFM) methods using a human autoimmune antibody directed against centrosomes (SPJ), and a mouse monoclonal antibody directed against tubulin (E7). Chromosomes were affected most by this treatment: premature chromosome condensation was detected in interphase nuclei, and the structure in mitotic chromosomes was altered compared to control cells. This would support previous findings in dividing sea urchin cells in which chromosomes are arrested at metaphase while the centrosome splitting cycle continues. It might also support findings that certairt-sulfhydryl-blocking agents block cyclin destruction. The organization of the microtubule network was scattered probably due to a looser organization of centrosomal material at the interphase centers and at the mitotic poles.

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