Chemically-defined medium for growth and differentiation of mixed epithelial and connective tissues in organ culture

In Vitro ◽  
1976 ◽  
Vol 12 (6) ◽  
pp. 450-459 ◽  
Author(s):  
Gisele M. Hodges ◽  
Anthony H. Melcher
Keyword(s):  
Organ Culture ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Connective Tissues

scholarly journals In vitro mouse spermatogenesis with an organ culture method in chemically defined medium

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192884 ◽  
Author(s):  
Hiroyuki Sanjo ◽  
Mitsuru Komeya ◽  
Takuya Sato ◽  
Takeru Abe ◽  
Kumiko Katagiri ◽  
...  
Keyword(s):  
Organ Culture ◽  
Culture Method ◽  
Defined Medium ◽  
Chemically Defined Medium

Chemically defined medium for organ culture differentiation of rat pancreas anlage

1970 ◽  
Vol 59 (1) ◽  
pp. 171-175 ◽  
Author(s):  
I. Parsa ◽  
W.H. Marsh ◽  
P.J. Fitzgerald
Keyword(s):  
Organ Culture ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Rat Pancreas

Endocytosis of Sugars in Embryonic Skeletal Tissues in Organ Culture

1969 ◽  
Vol 4 (1) ◽  
pp. 133-137
Author(s):  
T. C. APPLETON ◽  
S. R. PELC ◽  
J. T. DINGLE ◽  
HONOR B. FELL
Keyword(s):  
Organ Culture ◽  
Defined Medium ◽  
Water Soluble ◽  
Chemically Defined Medium ◽  
Limb Bone ◽  
Pinocytotic Vesicles ◽  
And Cartilage

Earlier experiments have shown that when limb-bone rudiments from 8½-day embryonic chicks are cultivated in medium containing an indigestible sugar, the perichondrial cells become intensely vacuolated, but this vacuolation does not appear in the chondrocytes, except in the articular region. Appleton's radioautographic method for demonstrating water-soluble materials was used to study the uptake, distribution and loss of 14C-labelled sucrose in 8½ limb-bone rudiments cultivated in a chemically defined medium (BGJ5). After 48 h exposure to [14C] sucrose, both perichondrium and cartilage were heavily labelled. When such explants were either washed for 1 h in BGJ5 or cultivated for a further 24 h in non-radioactive medium, labelling was almost unaffected in the perichondrial cells but was greatly reduced in the cartilage, the cells of which were almost devoid of radioactivity. The presence in the medium of 0.08 M ‘cold’ sucrose did not alter the perichondrial grain counts in the washed explants. This finding supports the view that in the perichondrial cells sucrose is taken up non-specifically by endocytosis, arid that the intense vacuolation of the cytoplasm is due to abnormal persistence of the pinocytotic vesicles in the presence of the sugar. It is concluded that failure of the chondrocytes to take up sucrose is due, not to lack of penetration of the sugar into the cartilage, but to a weak endocytotic activity of the cells.

Calf articular cartilage in organ culture in a chemically defined medium

In Vitro ◽  
1975 ◽  
Vol 11 (5) ◽  
pp. 286-290 ◽  
Author(s):  
Axel Bergenholtz ◽  
Rudolf K. Lemperg
Keyword(s):  
Articular Cartilage ◽  
Organ Culture ◽  
Defined Medium ◽  
Chemically Defined Medium

scholarly journals Organ Culture of Human Skin in Chemically Defined Medium

1975 ◽  
Vol 64 (2) ◽  
pp. 96-99 ◽  
Author(s):  
B.A. Flaxman ◽  
R.A. Harper
Keyword(s):  
Organ Culture ◽  
Human Skin ◽  
Defined Medium ◽  
Chemically Defined Medium

Calf articular cartilage in organ culture in a chemically defined medium

In Vitro ◽  
1975 ◽  
Vol 11 (5) ◽  
pp. 291-301 ◽  
Author(s):  
Rudolf K. Lemperg ◽  
Axel Bergenholtz ◽  
T. W. D. Smith
Keyword(s):  
Articular Cartilage ◽  
Organ Culture ◽  
Defined Medium ◽  
Chemically Defined Medium

Organ Culture of Postnatal Rat Ovaries in Chemically Defined Medium

1968 ◽  
Vol 19 (3) ◽  
pp. 317-338 ◽  
Author(s):  
Theodore Fainstat
Keyword(s):  
Organ Culture ◽  
Defined Medium ◽  
Chemically Defined Medium
Sign in / Sign up

Export Citation Format

Share Document