Quantitative assessment of the mutagenic effect of ethyl methanesulfonate inMicrococcus glutamicus

1972 ◽  
Vol 17 (2) ◽  
pp. 81-87 ◽  
Author(s):  
M. S. Shanthamma ◽  
Richard Joseph ◽  
T. N. Ramachandra Rao
Keyword(s):  
Quantitative Assessment ◽  
Mutagenic Effect ◽  
Ethyl Methanesulfonate

The mutagenic effect of ethyl methanesulfonate on a non-acid-fast strain ofMycobacterium phlei

1969 ◽  
Vol 14 (3) ◽  
pp. 194-200 ◽  
Author(s):  
J. Koníček ◽  
I. Málek
Keyword(s):  
Mutagenic Effect ◽  
Ethyl Methanesulfonate ◽  
Strain Ofmycobacterium

The mutagenic effect of prolonged treatment with ethyl methanesulfonate

1967 ◽  
Vol 4 (4) ◽  
pp. 409-413 ◽  
Author(s):  
J. Nečásek ◽  
P. Pikálek ◽  
J. Drobník
Keyword(s):  
Mutagenic Effect ◽  
Ethyl Methanesulfonate ◽  
Prolonged Treatment

Quantitative Assessment of HCV Load in Chronic Hemodialysis Patients

1999 ◽  
Vol 20 (02) ◽  
pp. 123
Author(s):  
Gina Pugliese ◽  
Martin S. Favero
Keyword(s):  
Quantitative Assessment ◽  
Hemodialysis Patients ◽  
Chronic Hemodialysis

Author response for "Quantitative assessment of amygdala in macaca fascicularis monkeys"

2020 ◽  
Author(s):  
José‐Carlos Delgado‐González ◽  
Carlos‐de‐la Rosa Prieto ◽  
Nuria Vallejo‐Calcerrada ◽  
Diana‐Lucía Tarruela‐Hernández ◽  
Sandra Cebada‐Sánchez ◽  
...  
Keyword(s):  
Quantitative Assessment ◽  
Macaca Fascicularis ◽  
Author Response

Deformation of Yeast Plasma Membrane by Ethidium Bromide

1988 ◽  
Vol 46 ◽  
pp. 254-255
Author(s):  
E. Keyhani
Keyword(s):  
Plasma Membrane ◽  
Thin Section ◽  
Ethidium Bromide ◽  
Freeze Fracture ◽  
Mutagenic Effect ◽  
Yeast Cells ◽  
Hydrophobic Region ◽  
Thin Section Electron Microscopy ◽  
Section Electron ◽  
Deep Pits

The mutagenic effect of ethidium bromide on the mitochondrial DNA is well established. Using thin section electron microscopy, it was shown that when yeast cells were grown in the presence of ethidium bromide, besides alterations in the mitochondria, the plasma membrane also showed alterations consisting of 75 to 110 nm-deep pits. Furthermore, ethidium bromide induced an increase in the length and number of endoplasmic reticulum and in the number of intracytoplasmic vesicles.Freeze-fracture, by splitting the hydrophobic region of the membrane, allows the visualization of the surface view of the membrane, and consequently, any alteration induced by ethidium bromide on the membrane can be better examined by this method than by the thin section method.Yeast cells, Candida utilis. were grown in the presence of 35 μM ethidium bromide. Cells were harvested and freeze-fractured according to the procedure previously described.

Initial experience with a new quantitative assessment tool for fluorescent imaging in peripheral artery disease

VASA ◽  
2017 ◽  
Vol 46 (5) ◽  
pp. 383-388 ◽  
Author(s):  
Henrik Christian Rieß ◽  
Anna Duprée ◽  
Christian-Alexander Behrendt ◽  
Tilo Kölbel ◽  
Eike Sebastian Debus ◽  
...  
Keyword(s):  
Indocyanine Green ◽  
Quantitative Assessment ◽  
Tissue Perfusion ◽  
Fluorescent Imaging ◽  
Peripheral Artery ◽  
Peripheral Bypass ◽  
Before And After ◽  
Artery Disease ◽  
Green Fluorescent ◽  
Peripheral Bypass Grafting

Abstract. Background: Perioperative evaluation in peripheral artery disease (PAD) by common vascular diagnostic tools is limited by open wounds, medial calcinosis or an altered collateral supply of the foot. Indocyanine green fluorescent imaging (ICG-FI) has recently been introduced as an alternative tool, but so far a standardized quantitative assessment of tissue perfusion in vascular surgery has not been performed for this purpose. The aim of this feasibility study was to investigate a new software for quantitative assessment of tissue perfusion in patients with PAD using indocyanine green fluorescent imaging (ICG-FI) before and after peripheral bypass grafting. Patients and methods: Indocyanine green fluorescent imaging was performed in seven patients using the SPY Elite system before and after peripheral bypass grafting for PAD (Rutherford III-VI). Visual and quantitative evaluation of tissue perfusion was assessed in an area of low perfusion (ALP) and high perfusion (AHP), each by three independent investigators. Data assessment was performed offline using a specially customized software package (Institute for Laser Technology, University Ulm, GmbH). Slope of fluorescent intensity (SFI) was measured as time-intensity curves. Values were compared to ankle-brachial index (ABI), slope of oscillation (SOO), and time to peak (TTP) obtained from photoplethysmography (PPG). Results: All measurements before and after surgery were successfully performed, showing that ABI, TTP, and SOO increased significantly compared to preoperative values, all being statistically significant (P < 0.05), except for TTP (p = 0.061). Further, SFI increased significantly in both ALP and AHP (P < 0.05) and correlated considerably with ABI, TTP, and SOO (P < 0.05). Conclusions: In addition to ABI and slope of oscillation (SOO), the ICG-FI technique allows visual assessment in combination with quantitative assessment of tissue perfusion in patients with PAD. Ratios related to different perfusion patterns and SFI seem to be useful tools to reduce factors disturbing ICG-FI measurements.

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