DNA — protein interactions during replication of Genetic elements of bacteria

1985 ◽  
Vol 30 (2) ◽  
pp. 154-176 ◽  
Author(s):  
J. Nešvera ◽  
J. Hochmannová
Keyword(s):  
Protein Interactions ◽  
Genetic Elements ◽  
Dna Protein Interactions

Tail labelled oligonucleotide probes for the detection of DNA-protein interactions

2011 ◽  
Author(s):  
Hana Pivoňková ◽  
Kateřina Němcová ◽  
Petra Horáková ◽  
Luděk Havran ◽  
Hana Macíčková-Cahová ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Oligonucleotide Probes ◽  
Dna Protein Interactions

scholarly journals Site-directed chemical modification for probing DNA-protein interactions

1989 ◽  
Vol 264 (35) ◽  
pp. 21277-21285
Author(s):  
P T Chan ◽  
J K Sullivan ◽  
J Lebowitz
Keyword(s):  
Chemical Modification ◽  
Protein Interactions ◽  
Dna Protein Interactions

Multiple DNA-protein interactions at the CpG island of the human pseudoautosomal geneMIC2

1993 ◽  
Vol 19 (1) ◽  
pp. 51-63 ◽  
Author(s):  
A. Braghetti ◽  
G. Piazzi ◽  
L. Lanfranco ◽  
C. Mondello
Keyword(s):  
Protein Interactions ◽  
Cpg Island ◽  
Dna Protein Interactions

scholarly journals A yeast one-hybrid system to detect methylation-dependent DNA–protein interactions

2004 ◽  
Vol 313 (4) ◽  
pp. 922-925 ◽  
Author(s):  
Shu-Ying Feng ◽  
Kazuhisa Ota ◽  
Yoichi Yamada ◽  
Norio Sawabu ◽  
Takashi Ito
Keyword(s):  
Hybrid System ◽  
Protein Interactions ◽  
Dna Protein Interactions

scholarly journals Complete WO Phage Sequences Reveal Their Dynamic Evolutionary Trajectories and Putative Functional Elements Required for Integration into the Wolbachia Genome

2009 ◽  
Vol 75 (17) ◽  
pp. 5676-5686 ◽  
Author(s):  
Kohjiro Tanaka ◽  
Seiichi Furukawa ◽  
Naruo Nikoh ◽  
Tetsuhiko Sasaki ◽  
Takema Fukatsu
Keyword(s):  
Genetic Transformation ◽  
Protein Interactions ◽  
Phylogenetic Analyses ◽  
Attachment Site ◽  
Host Bacterium ◽  
Sequence Motifs ◽  
Integrase Gene ◽  
Bacterial Phyla ◽  
Genetic Elements ◽  
Evolutionary Trajectories

ABSTRACT Wolbachia endosymbionts are ubiquitously found in diverse insects including many medical and hygienic pests, causing a variety of reproductive phenotypes, such as cytoplasmic incompatibility, and thereby efficiently spreading in host insect populations. Recently, Wolbachia-mediated approaches to pest control and management have been proposed, but the application of these approaches has been hindered by the lack of genetic transformation techniques for symbiotic bacteria. Here, we report the genome and structure of active bacteriophages from a Wolbachia endosymbiont. From the Wolbachia strain wCauB infecting the moth Ephestia kuehniella two closely related WO prophages, WOcauB2 of 43,016 bp with 47 open reading frames (ORFs) and WOcauB3 of 45,078 bp with 46 ORFs, were characterized. In each of the prophage genomes, an integrase gene and an attachment site core sequence were identified, which are putatively involved in integration and excision of the mobile genetic elements. The 3′ region of the prophages encoded genes with sequence motifs related to bacterial virulence and protein-protein interactions, which might represent effector molecules that affect cellular processes and functions of their host bacterium and/or insect. Database searches and phylogenetic analyses revealed that the prophage genes have experienced dynamic evolutionary trajectories. Genes similar to the prophage genes were found across divergent bacterial phyla, highlighting the active and mobile nature of the genetic elements. We suggest that the active WO prophage genomes and their constituent sequence elements would provide a clue to development of a genetic transformation vector for Wolbachia endosymbionts.

scholarly journals N6-methyl-adenine: an epigenetic signal for DNA–protein interactions

2006 ◽  
Vol 4 (3) ◽  
pp. 183-192 ◽  
Author(s):  
Didier Wion ◽  
Josep Casadesús
Keyword(s):  
Protein Interactions ◽  
Epigenetic Signal ◽  
Dna Protein Interactions

scholarly journals In vivo analysis of DNA-protein interactions on the human erythropoietin enhancer.

1997 ◽  
Vol 17 (2) ◽  
pp. 851-856 ◽  
Author(s):  
B Hu ◽  
E Wright ◽  
L Campbell ◽  
K L Blanchard
Keyword(s):  
Oxygen Tension ◽  
Protein Interactions ◽  
Proximal Promoter ◽  
Low Oxygen ◽  
Initiation Rate ◽  
Cobalt Exposure ◽  
In Cells ◽  
Dna Protein Interactions

The erythropoietin (EPO) gene is one of the best examples of a mammalian gene controlled by oxygen tension. The DNA elements responsible for hypoxia-induced transcription consist of a short region of the proximal promoter and a <50-bp 3' enhancer. The elements act cooperatively to increase the transcriptional initiation rate approximately 100-fold in response to low oxygen tension in Hep3B cells. Two distinct types of transactivating proteins have been demonstrated to bind the response elements in the human EPO enhancer in vitro: one shows hypoxia-inducible DNA binding activity, while the other activity binds DNA under normoxic and hypoxic conditions. We have investigated the DNA-protein interactions on the human EPO enhancer in living tissue culture cells that produce EPO in a regulated fashion (Hep3B) and in cells that do not express EPO under any conditions tested (HeLa). We have identified in vivo DNA-protein interactions on the control elements in the human EPO enhancer by ligation-mediated PCR technology. We show that the putative protein binding sites in the EPO enhancer are occupied in vivo under conditions of normoxia, hypoxia, and cobalt exposure in EPO-producing cells. These sites are not occupied in cells that do not produce EPO. We also provide evidence for a conformational change in the topography of the EPO enhancer in response to hypoxia and cobalt exposure.

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