Docichol alters GABA uptake and high affinity binding of agonist to rat brain synaptic plasma membranes

1989 ◽  
Vol 11 (2) ◽  
pp. 77-86 ◽  
Author(s):  
Joanna Strosznajder ◽  
Marek Samochocki
Keyword(s):  
Rat Brain ◽  
Plasma Membranes ◽  
Gaba Uptake ◽  
Affinity Binding ◽  
Synaptic Plasma Membranes ◽  
High Affinity Binding ◽  
High Affinity

High affinity binding of a fungal oligopeptide elicitor to parsley plasma membranes triggers multiple defense responses

Cell ◽  
1994 ◽  
Vol 78 (3) ◽  
pp. 449-460 ◽  
Author(s):  
Thorsten Nürnberger ◽  
Dirk Nennstiel ◽  
Thorsten Jabs ◽  
Wendy R. Sacks ◽  
Klaus Hahlbrock ◽  
...  
Keyword(s):  
Plasma Membranes ◽  
Defense Responses ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity

High affinity binding of 3H-Histamine in rat brain

1978 ◽  
Vol 50 (4) ◽  
pp. 443-444 ◽  
Author(s):  
José-María Palacios ◽  
Jean-Charles Schwartz ◽  
Monique Garbarg
Keyword(s):  
Rat Brain ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity

scholarly journals [3H]-ouabain binding sites in rat brain: distribution and properties assessed by quantitative autoradiography.

1992 ◽  
Vol 40 (6) ◽  
pp. 771-779 ◽  
Author(s):  
A A Maki ◽  
D G Baskin ◽  
W L Stahl
Keyword(s):  
Nervous System ◽  
Rat Brain ◽  
Cardiac Glycoside ◽  
Binding Sites ◽  
Quantitative Autoradiography ◽  
Affinity Binding ◽  
Ouabain Binding ◽  
High Affinity Binding ◽  
High Affinity ◽  
Kd Values

The anatomic distribution of high- and low-affinity cardiac glycoside binding sites in the nervous system is largely unknown. In the present study the regional distribution and properties of these sites were determined in rat brain by quantitative autoradiography (QAR). Two populations of cardiac glycoside binding sites were demonstrated with [3H]-ouabain, a specific inhibitor of Na,K-ATPases: (a) high-affinity binding sites with Kd values of 22-69 nM, which were blocked by erythrosin B, and (b) low-affinity binding sites with Kd values of 727-1482 nM. Sites with very low affinity for ouabain were not found by QAR. High- and low-affinity [3H]-ouabain binding sites were both found in all brain regions studied, including somatosensory cortex, thalamic and hypothalamic areas, medial forebrain bundle, amygdaloid nucleus, and caudate-putamen, although the distributions of high- and low-affinity sites were not congruent. Low-affinity [3H]-ouabain binding sites (Bmax = 222-358 fmol/mm2) were approximately twofold greater in number than high-affinity binding sites (Bmax = 76-138 fmol/mm2) in these regions of brain. Binding of [3H]-ouabain to both high- and low-affinity sites was blocked by Na+; however, low-affinity binding sites were less sensitive to inhibition by K+ (IC50 = 6.4 mM) than the high-affinity [3H]-ouabain binding sites (IC50 = 1.4 mM). The QAR method, utilizing [3H]-ouabain under standard conditions, is a valid method for studying modulation of Na,K-ATPase molecules in well-defined anatomic regions of the nervous system.

High-affinity binding of [3H]muscimol to subcellular particles of a neurone-enriched culture of embryonic rat brain

1979 ◽  
Vol 14 (2-3) ◽  
pp. 195-199 ◽  
Author(s):  
F.V. DeFeudis ◽  
Lucienne Ossola ◽  
G. Schmitt ◽  
P. Mandel
Keyword(s):  
Rat Brain ◽  
Affinity Binding ◽  
High Affinity Binding ◽  
High Affinity

High affinity binding site for γ-hydroxybutyric acid in rat brain

Life Sciences ◽  
1982 ◽  
Vol 30 (11) ◽  
pp. 953-961 ◽  
Author(s):  
J. Benavides ◽  
J.F. Rumigny ◽  
J.J. Bourguignon ◽  
C. Cash ◽  
C.G. Wermuth ◽  
...  
Keyword(s):  
Binding Site ◽  
Rat Brain ◽  
Affinity Binding ◽  
Hydroxybutyric Acid ◽  
High Affinity Binding ◽  
High Affinity ◽  
High Affinity Binding Site
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