Cloning and functional analysis of the novel rice blast resistance gene Pi65 in japonica rice

2021 ◽  
Author(s):  
Lili Wang ◽  
Zuobin Ma ◽  
Houxiang Kang ◽  
Shuang Gu ◽  
Zhanna Mukhina ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Japonica Rice ◽  
The Novel ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene

scholarly journals Cloning and Function Analysis of a Novel Rice Blast Resistance Gene Pi65 in Japonica Rice

2021 ◽  
Author(s):  
Lili Wang ◽  
Zuobin Ma ◽  
Houxiang Kang ◽  
Shuang Gu ◽  
Zhanna Mukhina ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Rice Blast Disease ◽  
Blast Disease ◽  
Resistant Variety ◽  
Japonica Rice ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene

Abstract Rice blast seriously threatens rice production worldwide. Utilizing the rice blast resistance gene to breed the rice blast resistant varieties is one of the best ways to control rice blast disease. Using a map-based cloning strategy, here, we cloned a novel rice blast resistance gene, Pi65 from the resistant variety GangYu129 (abbreviated GY129, O. sativa japonica ). Overexpression of Pi65 in the susceptible variety LiaoXing1 (abbreviated LX1, O. sativa japonica ) enhanced rice blast resistance, while knockout of Pi65 in GY129 resulted in susceptible to rice blast disease. Pi65 encodes two transmembrane domains, with 15 LRR domains and one serine/threonine protein kinase catalytic domain, conferring resistance to isolates of M. oryzae collected from northeast China. There are sixteen amino acids differences between the Pi65 resistance and susceptible alleles. Compared with the Pi65 resistant allele, the susceptible allele deleted one LRR domain. Pi65 was constitutively expressed in whole plants, and it could be induce expressed in the early stage of M. oryzae infection . Transcriptome analysis revealed that numerous genes associated with disease resistance were specifically upregulated in GY129 24-hour post inoculation (HPI), on the contrary, the photosynthesis-and carbohydrate metabolism-related genes were particularly downregulated 24 HPI, demonstrating that the disease resistance associated genes has been activated in GY129 (carrying Pi65 ) after rice blast fungal infection, and the cellular basal and energy metabolism was inhibited simultaneously. Our study provides genetic resources for improving rice blast resistance as well as enriches the study of rice blast resistance mechanisms.

scholarly journals Functional Analysis of Pid3-A4, an Ortholog of Rice Blast Resistance Gene Pid3 Revealed by Allele Mining in Common Wild Rice

2013 ◽  
Vol 103 (6) ◽  
pp. 594-599 ◽  
Author(s):  
Qiming Lv ◽  
Xiao Xu ◽  
Junjun Shang ◽  
Guanghuai Jiang ◽  
Zhiqian Pang ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Resistance Gene ◽  
Wild Rice ◽  
Rice Blast ◽  
Blast Resistance ◽  
Oryza Rufipogon ◽  
Allele Mining ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Common Wild Rice

The rice blast resistance gene Pid3 encodes a nucleotide-binding-site leucine-rich repeat (NBS-LRR) protein. This gene was cloned from the rice ‘Digu’ (indica) by performing a genome-wide comparison of the NBS-LRR gene family between two genome-sequenced varieties, ‘9311’ (indica) and ‘Nipponbare’ (japonica). In this study, we performed functional analysis of Pid3-A4, an ortholog of Pid3 revealed by allele mining in the common wild rice A4 (Oryza rufipogon). The predicted protein encoded by Pid3-A4 shares 99.03% sequence identity with Pid3, with only nine amino-acid substitutions. In wild rice plants, Pid3-A4 is constitutively expressed, and its expression is not induced by Magnaporthe oryzae isolate Zhong-10-8-14 infection. Importantly, in transgenic plants, Pid3-A4, as compared with Pid3, displays a distinct resistance spectrum to a set of M. oryzae isolates, including those that prevail in the rice fields of Sichuan Province. Therefore, Pid3-A4 should be quite useful for the breeding of rice blast resistance, especially in southwestern China.

Marker Development for Rice Blast Resistance Gene Pi66(t) and Application in the USDA Rice Mini-Core Collection

Crop Science ◽  
2016 ◽  
Vol 56 (3) ◽  
pp. 1001-1008 ◽  
Author(s):  
Yan Liu ◽  
Yulin Jia ◽  
David Gealy ◽  
David M. Goad ◽  
Ana L. Caicedo ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Rice Blast ◽  
Core Collection ◽  
Blast Resistance ◽  
Marker Development ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Mini Core Collection

scholarly journals Efficient authentic fine mapping of the rice blast resistance gene Pik-h in the Pik cluster, using new Pik-h-differentiating isolates

2008 ◽  
Vol 22 (2) ◽  
pp. 289-299 ◽  
Author(s):  
Xin Xu ◽  
N. Hayashi ◽  
C. T. Wang ◽  
H. Kato ◽  
T. Fujimura ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Fine Mapping ◽  
Rice Blast ◽  
Blast Resistance ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene

scholarly journals Identification of novel alleles of the rice blast resistance gene Pi54

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Kumar Vasudevan ◽  
Wilhelm Gruissem ◽  
Navreet K. Bhullar
Keyword(s):  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Phosphorylation Site ◽  
Allelic Diversity ◽  
Resistance Breeding ◽  
Reference Sequence ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene ◽  
Base Pair Deletion

Abstract Rice blast is one of the most devastating rice diseases and continuous resistance breeding is required to control the disease. The rice blast resistance gene Pi54 initially identified in an Indian cultivar confers broad-spectrum resistance in India. We explored the allelic diversity of the Pi54 gene among 885 Indian rice genotypes that were found resistant in our screening against field mixture of naturally existing M. oryzae strains as well as against five unique strains. These genotypes are also annotated as rice blast resistant in the International Rice Genebank database. Sequence-based allele mining was used to amplify and clone the Pi54 allelic variants. Nine new alleles of Pi54 were identified based on the nucleotide sequence comparison to the Pi54 reference sequence as well as to already known Pi54 alleles. DNA sequence analysis of the newly identified Pi54 alleles revealed several single polymorphic sites, three double deletions and an eight base pair deletion. A SNP-rich region was found between a tyrosine kinase phosphorylation site and the nucleotide binding site (NBS) domain. Together, the newly identified Pi54 alleles expand the allelic series and are candidates for rice blast resistance breeding programs.

Functional complementation of rice blast resistance gene Pi-k h (Pi54) conferring resistance to diverse strains of Magnaporthe oryzae

2011 ◽  
Vol 20 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Amit Kumar Rai ◽  
Satya Pal Kumar ◽  
Santosh Kumar Gupta ◽  
Naveen Gautam ◽  
Nagendera Kumar Singh ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Blast Resistance ◽  
Functional Complementation ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene

A Telomeric Avirulence Gene Determines Efficacy for the Rice Blast Resistance Gene Pi-ta

2000 ◽  
Vol 12 (11) ◽  
pp. 2019 ◽  
Author(s):  
Marc J. Orbach ◽  
Leonard Farrall ◽  
James A. Sweigard ◽  
Forrest G. Chumley ◽  
Barbara Valent
Keyword(s):  
Resistance Gene ◽  
Rice Blast ◽  
Blast Resistance ◽  
Avirulence Gene ◽  
Rice Blast Resistance ◽  
Blast Resistance Gene
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