Genetic analysis of shoot regeneration from cotyledonary explants in Brassica napus

2000 ◽  
Vol 100 (6) ◽  
pp. 895-898 ◽  
Author(s):  
Y. Ono ◽  
Y. Takahata
Keyword(s):  
Brassica Napus ◽  
Genetic Analysis ◽  
Shoot Regeneration ◽  
Cotyledonary Explants

Effect of genotype on shoot regeneration from cotyledonary explants of rapeseed (Brassica napus L.)

1994 ◽  
Vol 14 (1) ◽  
Author(s):  
Yuji Ono ◽  
Yoshihito Takahata ◽  
Norihiko Kaizuma
Keyword(s):  
Brassica Napus ◽  
Shoot Regeneration ◽  
Brassica Napus L ◽  
Cotyledonary Explants

scholarly journals The influence of silver thiosulfate and thidiazuron on shoot regeneration from cotyledon explants of Brassica napus

2012 ◽  
Vol 39 (3) ◽  
pp. 133-139 ◽  
Author(s):  
Kyung-Hee Roh ◽  
Bo-Kyung Kwak ◽  
Jong-Bum Kim ◽  
Kyeong-Ryeol Lee ◽  
Hyun-Uk Kim ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Shoot Regeneration ◽  
Silver Thiosulfate ◽  
Cotyledon Explants

scholarly journals Efficient Protoplast Regeneration Protocol and CRISPR/Cas9-Mediated Editing of Glucosinolate Transporter (GTR) Genes in Rapeseed (Brassica napus L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Xueyuan Li ◽  
Sjur Sandgrind ◽  
Oliver Moss ◽  
Rui Guan ◽  
Emelie Ivarson ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Shoot Regeneration ◽  
Gene Editing ◽  
Protoplast Culture ◽  
Callus Formation ◽  
Protoplast Regeneration ◽  
Induction Medium ◽  
Shoot Induction ◽  
Brassica Napus L ◽  
High Concentrations

Difficulty in protoplast regeneration is a major obstacle to apply the CRISPR/Cas9 gene editing technique effectively in research and breeding of rapeseed (Brassica napus L.). The present study describes for the first time a rapid and efficient protocol for the isolation, regeneration and transfection of protoplasts of rapeseed cv. Kumily, and its application in gene editing. Protoplasts isolated from leaves of 3–4 weeks old were cultured in MI and MII liquid media for cell wall formation and cell division, followed by subculture on shoot induction medium and shoot regeneration medium for shoot production. Different basal media, types and combinations of plant growth regulators, and protoplast culture duration on each type of media were investigated in relation to protoplast regeneration. The results showed that relatively high concentrations of NAA (0.5 mg l−1) and 2,4-D (0.5 mg l−1) in the MI medium were essential for protoplasts to form cell walls and maintain cell divisions, and thereafter auxin should be reduced for callus formation and shoot induction. For shoot regeneration, relatively high concentrations of cytokinin were required, and among all the combinations tested, 2.2 mg l−1 TDZ in combination with auxin 0.5 mg l−1 NAA gave the best result with up to 45% shoot regeneration. Our results also showed the duration of protoplast culture on different media was critical, as longer culture durations would significantly reduce the shoot regeneration frequency. In addition, we have optimized the transfection protocol for rapeseed. Using this optimized protocol, we have successfully edited the BnGTR genes controlling glucosinolate transport in rapeseed with a high mutation frequency.

Factors influencing shoot regeneration from cotyledonary explants ofCucumis melo

1989 ◽  
Vol 18 (3) ◽  
pp. 313-319 ◽  
Author(s):  
Randall P. Niedz ◽  
Sandra Schiller Smith ◽  
Kerry B. Dunbar ◽  
Christine T. Stephens ◽  
Harry H. Murakishi
Keyword(s):  
Shoot Regeneration ◽  
Factors Influencing ◽  
Cotyledonary Explants

Genetic Analysis of a Specially Long Pod Character in Artificially　Resythesized Brassica napus L.

2006 ◽  
Vol 28 (10) ◽  
pp. 1273 ◽  
Author(s):  
Yan-Hui WANG
Keyword(s):  
Brassica Napus ◽  
Genetic Analysis ◽  
Brassica Napus L

Development and primary genetic analysis of a fertility temperature-sensitive polima cytoplasmic male sterility restorer in Brassica napus

2007 ◽  
Vol 126 (3) ◽  
pp. 297-301 ◽  
Author(s):  
Z. X. Fan ◽  
W. X. Lei ◽  
D. F. Hong ◽  
J. P. He ◽  
L. L. Wan ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Genetic Analysis ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Temperature Sensitive

Effects of Genotype and AgNO3 on Shoot Regeneration in Winter Cultivars of Rapeseed (Brassica napus)

2008 ◽  
Vol 11 (16) ◽  
pp. 2040-2043 ◽  
Author(s):  
E.D. Uliaie ◽  
M. Farsi ◽  
B. Ghreyazie ◽  
J. Imani
Keyword(s):  
Brassica Napus ◽  
Shoot Regeneration
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